Zinc status, insulin resistance and glycoxidative stress in elderly subjects with type 2 diabetes mellitus

  • Authors:
    • Daniela Grădinaru
    • Denisa Margină
    • Anca Ungurianu
    • Georgiana Nițulescu
    • Cătălina Monica Pena
    • Constantin Ionescu‑Tîrgoviște
    • Rucsandra Dănciulescu Miulescu
  • View Affiliations

  • Published online on: September 30, 2021     https://doi.org/10.3892/etm.2021.10829
  • Article Number: 1393
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Abstract

Zinc deficiencies have been reported in numerous pathologies, such as diabetes mellitus, but also in the physiological process of ageing. Similarly, the end products of glycoxidation processes, advanced glycation end products (AGEs), are damaging compounds, a myriad of reports linking them to the development and progression of several age‑associated chronic diseases. The aim of the present study was to analyze the relationships between zinc status, glycoxidative stress and insulin resistance (IR) in elderly subjects with type 2 diabetes mellitus (T2DM). A group of 52 non‑smoking subjects (9 men and 43 women, aged 65‑83 years) were enrolled in this cross‑sectional study: 27 patients with T2DM, and 25 apparently healthy control subjects. Serum zinc (Zn) levels were assessed using a commercial kit based on an end‑point colorimetric method, and serum AGEs were evaluated with a fluorimetric analytic procedure. The calculated glucose‑to‑zinc ratio (Gly/Zn), insulin‑to‑zinc ratio (Ins/Zn) and insulin‑zinc resistance index (HOMA‑IR/Zn) were further used to study the associations between serum Zn levels, secretory function of β‑pancreatic cells and AGEs. T2DM patients presented significantly higher serum insulin and Zn levels, as compared to the controls. We found a significant inverse correlation between Zn and AGEs, and a strong positive correlation between AGEs and the Gly/Zn ratio, suggesting that both Zn and AGEs are biomarkers that could reflect the persistence of hyperglycemia. We identified new surrogate biomarkers useful for the assessment of glycemic control with great potential for the development of preventive and therapeutic strategies for elderly diabetics, based on the evaluation of serum Zn levels.

Introduction

Zinc (Zn) is one of the most important micronutrients, whose deficiency has a great impact on biological, biochemical and immune functions (1). The direct link between insulin secretion and function, and Zn concentration was established around 1930, Zn being identified as one of the ions inducing insulin crystallization as well as its action; later on it was shown that Zn is essential in the pancreatic β-cell for processing and storage of insulin (2).

Recent literature data demonstrate that the dysregulation of Zn metabolism is associated with an increased risk of diabetes, as well as the impairment of the immune response, the latter being itself a component of diabetes mellitus (DM) etiopathology. Zn physiology in the β-cell depends on the function of ZnT8, a transporter encoded by the SLC30A8 gene, whose polymorphism is associated with high type 2 DM (T2DM) risk (3). Among all the trace elements studied, the plasma Zn concentration seems to be a useful biomarker of ageing since its concentration has been shown to slowly decline with age in many different studies (4,5). At the same time, the intracellular concentration of metallothioneins (MTs), the main intracellular proteins involved in Zn homeostasis, was shown to increase during ageing and age-related diseases (6,7). Zn deficiencies are reported in the course of ageing and in different diseases, such as DM, obesity, atherosclerosis, as well as rheumatoid arthritis or respiratory tract infections, among others (2,8-14). In fact, the age-related changes of trace element concentrations in biological fluids/tissues are not only attributed to a deficient nutritional status, but also to an intrinsic dysregulated homeostasis (1).

At the systemic level, glycation and oxidative stress resulting from hyperglycemia and dyslipidemia lead to accelerated non-enzymatic modification of essential biomolecules, particularly of proteins (15-19). In particular, advanced glycation end products (AGEs) are represented by a heterogenous group of damaging compounds (e.g., pentosidine, carboxymethyllysine, and imidazolone) that are formed by nonenzymatic glycation of macromolecules; some of them have characteristic fluorescence, with the ability of crosslinking of protein and interacting with AGE-specific receptor RAGE (20-24). AGE accumulation in tissues reflects cumulative metabolic stress, or ‘metabolic memory’ rather than short-term glycemic control, thus pointing to protein tissue damage resulted from the effects of exposure to many cardiovascular disease (CVD) risk factors (17,25-27).

Despite the numerous studies, there is still limited information concerning the relationships between Zn status, glycoxidative stress and IR in elderly with impaired glucose metabolism. The aim of this pilot study was to evaluate serum Zn levels in elderly subjects with T2DM and analyze the biological and clinical relevance of this biomarker in terms of glycoxidative stress, metabolic profile and pancreatic function.

Patients and methods

Subjects

A total of 52 non-smoking elderly subjects (9 men and 43 women, aged 65-83 years, mean age: 70+5 years) were enrolled in this cross-sectional study: a T2DM group composed of 27 known type 2 diabetic patients treated at ‘Prof. Nicolae Paulescu’ INMD clinic department (during September 2019), and 25 apparently healthy control subjects selected from the patients admitted at Ana Aslan National Institute of Gerontology and Geriatrics (during September 2019), with normal glucose levels and normal lipid profile, recruited from the outpatient clinical department. Subjects were included in the T2DM study group, according to the clinical and biochemical criteria of the American Diabetes Association (28). Diabetic patients were on different oral antidiabetic medication, mainly metformin and gliclazide. Exclusion criteria included acute infection, acute myocardial infarction, active liver disease or liver dysfunction, renal impairment, hematological and malignant overt diseases. Patients who were on insulin or taking any antioxidant or Zn supplements were also excluded.

Biochemical parameters

A single blood sample (6 ml) was obtained from each subject after an 8-12 h overnight fasting. Serum samples were separated by centrifugation (3,000 x g for 10 min) from whole blood for the assessment of metabolic profile: glucose, total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), blood urea nitrogen, creatinine and uric acid, using standard methods on a Cobas C311 Analyzer and Cobas Integra 400 Analyzer for HbA1C (Roche Diagnostics Ltd., Switzerland). Fasting insulin levels were assessed with a Sandwich ELISA based assay (EIA-2935, DRG Diagnostics). The inter-assay coefficient of variation (CV) was 6.0%, and the intra-assay CV was 2.6%. Insulin resistance (IR) was evaluated using the homeostasis model assessment-insulin resistance (HOMA-IR): fasting insulin (mU/l) x fasting glucose (mg/dl) divided by 405(29). The serum AGEs were assessed by the level of AGE-associated autofluorescence recorded at 440 nm emission wavelength upon excitation at 350 nm, as previously described (17). The measurements were performed on PerkinElmer Spectrofluorimeter (LS 50 B, PerkinElmer, Inc.). Fluorescence intensity was expressed in relative fluorescence units (RFU). The coefficient of variation (CV) for replicate measurements was <5%. Zn assessment in serum samples was performed using a sensitive, standardized, simple and direct procedure (reagent kit MAK032, Sigma-Aldrich; Merck KGaA) which measures the colorimetric product (560 nm) resulting from the binding of Zn ions to a specific ligand, namely 5-Bromo-PAPS [2-(5-Bromo-2-pyridylazo)-5-[N-propyl-N-(3-sulfopropyl)amino] phenol disodium salt dihydrate]. Concentration of serum Zn was expressed in mM Zn (mmol/l serum). The inter-assay and intra-assay coefficients of variation were 5.4 and 6.7%, respectively. Biochemical and immunoenzymatic tests were performed on ChemWell 2190 Analyzer (Awareness Technology).

Indices of glycemic control and Zn status

The glucose-to-zinc ratio (Gly/Zn) was calculated using the fasting levels of serum glucose and Zn, expressed both as mmol/l serum. The insulin-to-zinc ratio (Ins/Zn) was calculated using the fasting levels of serum insulin (mU/l serum) and Zn, expressed as mmol/l serum. The insulin-zinc resistance index (HOMA-IR/Zn) was calculated using HOMA-IR individual values and serum Zn levels.

Statistical analysis

We used the Statistical Package for Social Sciences software (SPSS, version 15, SPSS, Inc.). Comparison between the control group and T2DM group was performed using Student's unpaired t-test. Pearson's two-tailed bivariate correlations (r) were performed to examine the associations of serum Zn and serum AGEs, with insulin, glucose, HOMA-IR, as well as the correlations between AGEs and Gly/Zn, Ins/Zn and HOMA-IR/Zn ratios. Both tests were two-sided and a P-value <0.05 was considered statistically significant.

Results

The study population included 52 elderly subjects divided into two groups: a control group, with normal metabolic profile (n=25), and a group with T2DM (n=27). The biochemical and metabolic parameters measured in both groups are presented in Table I.

Table I

Biochemical parameters in control and type 2 diabetes mellitus (T2DM) elderly subjects.

Table I

Biochemical parameters in control and type 2 diabetes mellitus (T2DM) elderly subjects.

ParametersControl group (n=25)T2DM group (n=27)P-value
Age, mean (years)69±571±5NS
Fasting glucose (mg/dl)98.92±10.99152.65±30.23<0.001
HbA1c (%)5.88±0.627.46±0.85<0.001
Fasting insulin (µU/ml)4.45±1.2111.49±4.31<0.001
HOMA-IR1.10±0.364.26±1.57<0.001
Total cholesterol (mg/dl)180.67±32.80198.96±33.94NS
Triglycerides (mg/dl)109.88±69.28167.69±64.52<0.01
HDL-cholesterol (mg/dl)55.83±14.7343.73±11.58<0.01
LDL-cholesterol (mg/dl)103.83±36.83119.92±30.86NS
Uric acid (mg/dl)4.42±1.675.47±1.62<0.05
Creatinine (mg/dl)0.86±0.160.92±0.16NS
Blood urea nitrogen (mg/dl)38.79±13.5043.27±15.88<0.01
AGEs (RFU)103.84±13.73120.74±21.47<0.01
Serum zinc (mmol/l)14.11±2.0615.71±1.71<0.01

[i] Values are expressed as mean ± SD; n, number of subjects. P<0.05 indicates statistical significance when compared to the control group; NS, not significant; HOMA-IR, homeostasis model assessment of insulin resistance; AGEs, advanced glycation end products.

The mean values of biomarkers indicating the metabolic dysregulation and cardiovascular risk, namely the values of fasting glucose, insulin, and triglycerides were significantly higher in the T2DM group compared with the control group, whereas HDL-cholesterol levels were significantly lower. Insignificant differences between groups were observed for creatinine, whereas uric acid and blood urea nitrogen levels were slightly higher in the T2DM group. T2DM elderly patients were characterized by a significant hyperinsulinemia and subsequent significant increases in insulin resistance index HOMA-IR.

The glycoxidative stress was evaluated as an AGE biomarker as well as the traditional marker indicating the persistence of chronic hyperglycemia, HbA1C; both were significantly higher in elderly subjects with T2DM vs. the control group.

Serum Zn levels were significantly (P<0.01) increased in the T2DM group as compared to the control subjects. The mean Zn value in the T2DM group was 15.7 mmol/l (range: 12.03-19.12 mmol/l), whereas in the control group the average serum Zn concentration was 14.11 mmol/l (range: 9.89-17.31 mmol/l).

Overall, elderly subjects with T2DM presented significantly higher serum insulin and Zn levels, as well as HOMA-IR values, as compared to the control. Therefore, the calculated Gly/Zn, Ins/Zn and insulin-zinc resistance index (HOMA-IR/Zn) were further used as surrogate biomarkers to explore the relationships between serum Zn levels, metabolic profile, secretory functions of β-pancreatic cells and glycoxidative stress. All of these three metabolic indices illustrative of the glycemic control and Zn status displayed significantly higher values in T2DM patients than in the control subjects (Fig. 1).

In T2DM patients we aimed to explore the relationships between the advanced glycation of proteins (AGEs), Zn systemic status and glucose homeostasis. The correlation analysis was carried out between levels of AGEs and serum Zn levels, Gly/Zn ratio, Ins/Zn ratio and HOMA-IR/Zn ratio. Statistical significance of the Pearson's correlation coefficients was different among the four study biomarkers as presented in Fig. 2.

AGEs values showed a significant negative correlation (r=-0.454; P<0.02) with Zn serum levels and a strong positive association (r=0.767; P<0.001) with the Gly/Zn ratio (Fig. 2A and B). Regarding the association between AGEs and β-cell secretory function, the correlation analysis identified significant negative relationships between AGEs and insulin (r=-0.459; P<0.02) and significantly higher between AGEs and Ins/Zn ratio (r=-0.523; P<0.01) (Fig. 2C). Despite the elevated levels of circulating AGEs found out in hyperglycemic and insulin-resistant subjects, the association with HOMA-IR was not significant (r=-0.145; NS), whereas the correlation between AGEs and HOMA-IR/Zn ratio was significant (r=-0.382; P<0.05) (Fig. 2D).

Discussion

The results of the present showed that elderly diabetic patients had significantly higher levels of serum Zn in comparison with the control group subjects. These results are in agreement with a recent study conducted in postmenopausal women with diabetes, characterized by significantly higher Zn levels as compared to the controls (30). However, these data are in contrast with many studies, conducted mainly on adult population, in which T2DM individuals displayed a reduced Zn status correlated with a poor glycemic control (31-33). In a recent systematic review and meta-analysis, Yin et al showed that the serum Zn concentration was lower in polycystic ovary syndrome (PCOS) women with IR than in healthy women. However, there was no significant difference in serum Zn concentration when compared between healthy control and PCOS women without IR (34).

Understanding the complex role of Zn in glucose homeostasis and subsequent IR and T2DM is at present limited by controversial findings in the literature and the absence of ‘gold standard’ animal models that could help elucidate the role of Zn and Zn transporters (35). It is acknowledged that, at the pancreatic level, Zn participates in mechanisms involving insulin secretion and action, as a catalytic cofactor for carboxypeptidase H, an enzyme which catalyzes the conversion from proinsulin into insulin. Zn also promotes phosphorylation of insulin receptor by enhancing glucose transport into cells (36). Previous studies have demonstrated the role of higher serum concentration of Zn in improving insulin sensitivity in T2DM (37). Regarding the roles of Zn in blood insulin levels, recent experimental studies conducted on mice with β-cell-specific genetic deficiency for ZnT8 transporter found an enhanced insulin secretion but also an enhanced insulin clearance in the liver, due to reduced Zn secretion from these cells (38).

In the present study, we also pointed out a significant inverse correlation between Zn levels and AGEs, and a strong positive correlation between the Gly/Zn ratios and AGEs, suggesting that both Zn and AGEs are biomarkers that could reflect the persistence of hyperglycemia. These results could also suggest that Zn could exert at the systemic level some anti-glycation actions, as confirmed in studies conducted in vitro (39). Therefore, we also made an attempt to ‘normalize’ the traditional indices of β-cells secretory function and IR to serum Zn levels and obtain new surrogate biomarkers, namely Ins/Zn and HOMA-IR/Zn ratios, and study their relationships with AGEs. In the elderly diabetic subjects, Ins/Zn and HOMA-IR/Zn ratios were significantly inversely correlated with the glycoxidative stress evaluated as AGEs.

The main limitation of the study was the reduced number of subjects and the absence of evaluation of proteins involved in Zn metabolism such as Zn-α2-glycoprotein and Zn-induced metallothionein, that could help to elucidate the mechanisms involved in the interplay between pancreatic β-cells/Zn/glycoxidative stress.

In the present study, we propose, for the first time, the evaluation of these new Zn status surrogate biomarkers involved in glucose homeostasis, as numerous clinical markers and quotients are routinely used to evaluate the metabolic impairment. Similarly, a recent study on patients with polycystic ovary syndrome (PCOS), T2DM and impaired glucose tolerance (IGT) identified the natural logarithm of Zn-α2-glycoprotein/HOMA-IR as a better predictor of insulin sensitivity than the product of triglycerides and glucose and the other lipid ratios (40). Therefore, we evaluated the biochemical and clinical relevance serum AGEs and the newly introduced ratios of Ins/Zn and HOMA-IR/Zn, which could be useful in clinical practice in the diagnosis of IR associated with Zn deficiency and oxidative stress.

In conclusion, in the present study conducted on elderly diabetic subjects, we identified new significant associations between Zn status, glycoxidative stress, pancreatic β-cell impairment and IR. Similar approaches could help in the future for the development of preventive strategies for personalized nutrition and treatment, that would increase the longevity and quality of life of patients with diabetes.

Acknowledgements

Not applicable.

Funding

The experiments carried out in this study and the publication of the article were supported by regular financial resources provided by ‘Carol Davila’ University of Medicine and Pharmacy within the general framework for the development of scientific research collaboration.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author.

Authors' contributions

DG and DM conceived and designed the study, contributed to the experiments and drafted the manuscript. AU, GN and CMP performed all the experiments and the statistical analysis. RDM performed the clinical data analysis. CMP, CIT and RDM contributed to the conception and designed the study. All the authors have read and approved the final manuscript for publication.

Ethics approval and consent to participate

The study protocol was approved by ‘Nicolae Paulescu’ Institute of Diabetes, Metabolic and Nutrition Diseases (no. 10403/2019) and ‘Ana Aslan’ National Institute of Gerontology and Geriatrics (no. 12660/2019) Ethics Committees and all subjects provided their informed consent before participation.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Spandidos Publications style
Grădinaru D, Margină D, Ungurianu A, Nițulescu G, Pena CM, Ionescu‑Tîrgoviște C and Dănciulescu Miulescu R: Zinc status, insulin resistance and glycoxidative stress in elderly subjects with type 2 diabetes mellitus. Exp Ther Med 22: 1393, 2021
APA
Grădinaru, D., Margină, D., Ungurianu, A., Nițulescu, G., Pena, C.M., Ionescu‑Tîrgoviște, C., & Dănciulescu Miulescu, R. (2021). Zinc status, insulin resistance and glycoxidative stress in elderly subjects with type 2 diabetes mellitus. Experimental and Therapeutic Medicine, 22, 1393. https://doi.org/10.3892/etm.2021.10829
MLA
Grădinaru, D., Margină, D., Ungurianu, A., Nițulescu, G., Pena, C. M., Ionescu‑Tîrgoviște, C., Dănciulescu Miulescu, R."Zinc status, insulin resistance and glycoxidative stress in elderly subjects with type 2 diabetes mellitus". Experimental and Therapeutic Medicine 22.6 (2021): 1393.
Chicago
Grădinaru, D., Margină, D., Ungurianu, A., Nițulescu, G., Pena, C. M., Ionescu‑Tîrgoviște, C., Dănciulescu Miulescu, R."Zinc status, insulin resistance and glycoxidative stress in elderly subjects with type 2 diabetes mellitus". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1393. https://doi.org/10.3892/etm.2021.10829