Diagnostic value and significance of serum miR‑132 combined with miR‑223 for sepsis‑induced cardiomyopathy

Li,Yanping; Lu,Bin; Yu,Muming; Zhai,Jianhua; Yao,Ying; Chai,Yanfen

doi:10.3892/etm.2021.10832

Diagnostic value and significance of serum miR‑132 combined with miR‑223 for sepsis‑induced cardiomyopathy

Authors:
- Yanping Li
- Bin Lu
- Muming Yu
- Jianhua Zhai
- Ying Yao
- Yanfen Chai
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Affiliations: Department of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China
Published online on: October 1, 2021 https://doi.org/10.3892/etm.2021.10832
Article Number: 1396
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In previous studies, miR‑132 and miR‑223 were considered to be involved in cellular and pathological processes of diseases. However, the role of early diagnosis and prognosis evaluation in sepsis‑induced cardiomyopathy (SIC) remains unknown. The present study aimed to explore the diagnostic value of combined detection of miR‑132 and miR‑223 for SIC and their correlation with creatine kinase‑MB (CK‑MB), cardiac troponin I (cTnI), tumor necrosis factor α (TNF‑α), and interleukin‑6 (IL)‑6. SIC patients (n=80) admitted to Tianjin Medical University General Hospital were assigned to the research group (RG), while 60 healthy participants receiving physical examinations at the same period were assigned to the control group (CG). Serum expression profiles of miR‑132 and miR‑223 were detected by the RT‑qPCR. CK‑MB and cTnI were assessed using chemiluminescence assay, and TNF‑α and IL‑6 by enzyme‑linked immunosorbent assay (ELISA). Serum miR‑132 and miR‑223 levels were significantly lower in the RG than in the CG (P<0.001). The sensitivity and specificity for the diagnosis of SIC were 82.50 and 71.67% for miR‑132, 95.00 and 61.67% for miR‑223, as well as 86.25 and 86.67% for miR‑132 combined with miR‑223. Serum miR‑132 and miR‑223 levels were significantly higher in the survivor group than in the deceased group (P<0.001). The sensitivity and specificity for the prognosis of SIC were 85.96 and 65.22% for miR‑132 combined with miR‑223. Serum miR‑132 and miR‑223 were negatively correlated with serum CK‑MB, cTnI, TNF‑α, and IL‑6 (P<0.001). miR‑132 combined with miR‑223 can be used for early diagnosis and prognostic evaluation of SIC, and the two are correlated with CK‑MB, cTnI, TNF‑α, and IL‑6.

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1	Sato R, Kuriyama A, Takada T, Nasu M and Luthe SK: Prevalence and risk factors of sepsis-induced cardiomyopathy: A retrospective cohort study. Medicine (Baltimore). 95(e5031)2016.PubMed/NCBI View Article : Google Scholar
2	Yang F, Zhao LN, Sun Y and Chen Z: Levosimendan as a new force in the treatment of sepsis-induced cardiomyopathy: Mechanism and clinical application. J Int Med Res. 47:1817–1828. 2019.PubMed/NCBI View Article : Google Scholar
3	Dombrovskiy VY, Martin AA, Sunderram J and Paz HL: Rapid increase in hospitalization and mortality rates for severe sepsis in the United States: A trend analysis from 1993 to 2003. Crit Care Med. 35:1244–1250. 2007.PubMed/NCBI View Article : Google Scholar
4	Russell JA and Walley KR: Update in sepsis 2012. Am J Respir Crit Care Med. 187:1303–1307. 2013.PubMed/NCBI View Article : Google Scholar
5	Semeraro N, Ammollo CT, Semeraro F and Colucci M: Sepsis, thrombosis and organ dysfunction. Thromb Res. 129:290–295. 2012.PubMed/NCBI View Article : Google Scholar
6	Hochstadt A, Meroz Y and Landesberg G: Myocardial dysfunction in severe sepsis and septic shock: More questions than answers? J Cardiothorac Vasc Anesth. 25:526–535. 2011.PubMed/NCBI View Article : Google Scholar
7	Romero-Bermejo FJ, Ruiz-Bailen M, Gil-Cebrian J and Huertos-Ranchal MJ: Sepsis-induced cardiomyopathy. Curr Cardiol Rev. 7:163–183. 2011.PubMed/NCBI View Article : Google Scholar
8	Sato R and Nasu M: A review of sepsis-induced cardiomyopathy. J Intensive Care. 3(48)2015.PubMed/NCBI View Article : Google Scholar
9	An R, Feng J, Xi C, Xu J and Sun L: miR-146a attenuates sepsis-induced myocardial dysfunction by suppressing IRAK1 and TRAF6 via targeting ErbB4 expression. Oxid Med Cell Longev. 2018(7163057)2018.PubMed/NCBI View Article : Google Scholar
10	Sluijter JP and Doevendans PA: Sepsis-associated cardiac dysfunction is controlled by small RNA molecules. J Mol Cell Cardiol. 97:67–69. 2016.PubMed/NCBI View Article : Google Scholar
11	Zheng G, Pan M, Jin W, Jin G and Huang Y: MicroRNA-135a is up-regulated and aggravates myocardial depression in sepsis via regulating p38 MAPK/NF-κB pathway. Int Immunopharmacol. 45:6–12. 2017.PubMed/NCBI View Article : Google Scholar
12	Qian Y, Song J, Ouyang Y, Han Q, Chen W, Zhao X, Xie Y, Chen Y, Yuan W and Fan C: Advances in roles of miR-132 in the nervous system. Front Pharmacol. 8(770)2017.PubMed/NCBI View Article : Google Scholar
13	Mancuso R, Agostini S, Hernis A, Zanzottera M, Bianchi A and Clerici M: Circulatory miR-223-3p discriminates between Parkinson's and Alzheimer's patients. Sci Rep. 9(9393)2019.PubMed/NCBI View Article : Google Scholar
14	Li M, Shao H, Zhang X and Qin B: Hesperidin alleviates lipopolysaccharide-induced neuroinflammation in mice by promoting the miRNA-132 pathway. Inflammation. 39:1681–1689. 2016.PubMed/NCBI View Article : Google Scholar
15	Wang JF, Yu ML, Yu G, Bian JJ, Deng XM, Wan XJ and Zhu KM: Serum miR-146a and miR-223 as potential new biomarkers for sepsis. Biochem Biophys Res Commun. 394:184–188. 2010.PubMed/NCBI View Article : Google Scholar
16	Wang H, Bei Y, Shen S, Huang P, Shi J, Zhang J, Sun Q, Chen Y, Yang Y, Xu T, et al: miR-21-3p controls sepsis-associated cardiac dysfunction via regulating SORBS2. J Mol Cell Cardiol. 94:43–53. 2016.PubMed/NCBI View Article : Google Scholar
17	Hashmat N, Shabbir I, Rahat T, Ijaz F and Majeed S: Clinical profile and disease outcome of septic patients at public sector hospital. Pak J Med Res. 54:44–47. 2015.
18	Zhuang YT, Xu DY, Wang GY, Sun JL, Huang Y and Wang SZ: IL-6 induced lncRNA MALAT1 enhances TNF-α expression in LPS-induced septic cardiomyocytes via activation of SAA3. Eur Rev Med Pharmacol Sci. 21:302–309. 2017.PubMed/NCBI
19	Zhao A, Li G, Péoc'h M, Genin C and Gigante M: Serum miR-210 as a novel biomarker for molecular diagnosis of clear cell renal cell carcinoma. Exp Mol Pathol. 94:115–120. 2013.PubMed/NCBI View Article : Google Scholar
20	Kakihana Y, Ito T, Nakahara M, Yamaguchi K and Yasuda T: Sepsis-induced myocardial dysfunction: Pathophysiology and management. J Intensive Care. 4(22)2016.PubMed/NCBI View Article : Google Scholar
21	Wang H, Bei Y, Huang P, Zhou Q, Shi J, Sun Q, Zhong J, Li X, Kong X and Xiao J: Inhibition of miR-155 protects against LPS-induced cardiac dysfunction and apoptosis in mice. Mol Ther Nucleic Acids. 5(e374)2016.PubMed/NCBI View Article : Google Scholar
22	Liu F, Li Y, Jiang R, Nie C, Zeng Z, Zhao N, Huang C, Shao Q, Ding C, Qing C, et al: miR-132 inhibits lipopolysaccharide-induced inflammation in alveolar macrophages by the cholinergic anti-inflammatory pathway. Exp Lung Res. 41:261–269. 2015.PubMed/NCBI View Article : Google Scholar
23	Essandoh K and Fan GC: Role of extracellular and intracellular microRNAs in sepsis. Biochim Biophys Acta. 1842:2155–2162. 2014.PubMed/NCBI View Article : Google Scholar
24	Nahid MA, Yao B, Dominguez-Gutierrez PR, Kesavalu L, Satoh M and Chan EK: Regulation of TLR2-mediated tolerance and cross-tolerance through IRAK4 modulation by miR-132 and miR-212. J Immunol. 190:1250–1263. 2013.PubMed/NCBI View Article : Google Scholar
25	Aziz F: The emerging role of miR-223 as novel potential diagnostic and therapeutic target for inflammatory disorders. Cell Immunol. 303:1–6. 2016.PubMed/NCBI View Article : Google Scholar
26	Wang X, Huang W, Yang Y, Wang Y, Peng T, Chang J, Caldwell CC, Zingarelli B and Fan GC: Loss of duplexmiR-223 (5p and 3p) aggravates myocardial depression and mortality in polymicrobial sepsis. Biochim Biophys Acta. 1842:701–711. 2014.PubMed/NCBI View Article : Google Scholar
27	Chen H, Wang X, Yan X, Cheng X, He X and Zheng W: LncRNA MALAT1 regulates sepsis-induced cardiac inflammation and dysfunction via interaction with miR-125b and p38 MAPK/NFκB. Int Immunopharmacol. 55:69–76. 2018.PubMed/NCBI View Article : Google Scholar
28	Liu J, Li J, Tian P, Guli B, Weng G, Li L and Cheng Q: H₂S attenuates sepsis-induced cardiac dysfunction via a PI3K/Akt-dependent mechanism. Exp Ther Med. 17:4064–4072. 2019.PubMed/NCBI View Article : Google Scholar
29	Qin YJ, Zhang XL, Yu YQ, Bian XH and Dong SM: Cardioprotective effect of erythropoietin on sepsis-induced myocardial injury in rats. World J Emerg Med. 4:215–222. 2013.PubMed/NCBI View Article : Google Scholar
30	Zhang Y, Han B, He Y, Li D, Ma X, Liu Q and Hao J: MicroRNA-132 attenuates neurobehavioral and neuropathological changes associated with intracerebral hemorrhage in mice. Neurochem Int. 107:182–190. 2017.PubMed/NCBI View Article : Google Scholar
31	Wang X, Gu H, Qin D, Yang L, Huang W, Essandoh K, Wang Y, Caldwell CC, Peng T, Zingarelli B and Fan GC: Exosomal miR-223 contributes to mesenchymal stem cell-elicited cardioprotection in polymicrobial sepsis. Sci Rep. 5(13721)2015.PubMed/NCBI View Article : Google Scholar