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Low‑doses of aspirin promote the growth of human PC‑9 lung cancer cells through activation of the MAPK family

  • Authors:
    • Yue Qian
    • Hongliang Dai
    • Hongyu Li
  • View Affiliations / Copyright

    Affiliations: Institute of Biological Sciences, Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, School of Nursing, Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Institute of Medical Education, Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China
    Copyright: © Qian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1440
    |
    Published online on: October 12, 2021
       https://doi.org/10.3892/etm.2021.10875
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Abstract

Aspirin has been reported for its anti‑tumor activity, however, there are few studies on its effects in lung cancer. The present study found that aspirin had a dual role in the proliferation of human lung cancer PC‑9 (formerly known as PC‑14) and A549 cells, and in human colon cancer HCT116 cells. The cells were treated with 0, 1, 2, 4, 8 and 16 mM aspirin for 24-72 h or 7‑12 days and cell proliferation was examined by MTT and colony formation assay. In order to explore the relationship between the proliferation‑enhancing effect of low‑dose aspirin and mitogen‑activated protein kinase (MAPK) signaling activation, PC‑9 cells were pretreated with 10 µM PD98059 (a specific inhibitor of ERK), SB203580 (a specific inhibitor of p38) and SP600125 (a specific inhibitor of JNK) for 30 min respectively. Western blot assay was performed to detect the activation of MAPK members in PC‑9 cells. Cellular apoptosis was detected using flow cytometer‑based Annexin V/propidium iodide dual staining. An assessment of MAPK inhibitors was performed to further validate the role of JNK, p38 and ERK in aspirin‑promoted PC‑9 cell growth. It was demonstrated that aspirin could promote the growth of human PC‑9 lung cancer cells and induced MAPK activation at low concentrations. All the MAPK inhibitors tested (PD98059, SB203580 and SP600125) were able to inhibit the aspirin‑induced proliferation of PC‑9 cells.
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1 

Zhang SW, Zheng RS, Yang ZX, Zeng HM, Sun KX, Gu XY, Li H, Chen WQ and He J: Trend analysis on incidence and age at diagnosis for lung cancer in cancer registration areas of China, 2000-2014. Zhonghua Yu Fang Yi Xue Za Zhi. 52:579–585. 2018.PubMed/NCBI View Article : Google Scholar : (In Chinese).

2 

Torre LA, Siegel RL and Jemal A: Lung Cancer Statistics. Adv Exp Med Biol. 893:1–19. 2016.PubMed/NCBI View Article : Google Scholar

3 

Wang X and Guo Z: The role of sulfur in platinum anticancer chemotherapy. Anticancer Agents Med Chem. 7:19–34. 2007.PubMed/NCBI View Article : Google Scholar

4 

Kim J and Becker RC: Aspirin dosing frequency in the primary and secondary prevention of cardiovascular events. J Thromb Thrombolysis. 41:493–504. 2016.PubMed/NCBI View Article : Google Scholar

5 

Okada S, Morimoto T, Ogawa H, Sakuma M, Matsumoto C, Soejima H, Nakayama M, Doi N, Jinnouchi H, Waki M, et al: Effect of aspirin on cancer chemoprevention in Japanese patients with type 2 diabetes: 10-Year observational follow-up of a randomized controlled trial. Diabetes Care. 41:1757–1764. 2018.PubMed/NCBI View Article : Google Scholar

6 

Hua H, Zhang H, Kong Q, Wang J and Jiang Y: Complex roles of the old drug aspirin in cancer chemoprevention and therapy. Med Res Rev. 39:114–145. 2019.PubMed/NCBI View Article : Google Scholar

7 

Di Francesco L, López Contreras LA, Sacco A and Patrignani P: New insights into the mechanism of action of aspirin in the prevention of colorectal neoplasia. Curr Pharm Des. 21:5116–5126. 2015.PubMed/NCBI View Article : Google Scholar

8 

Usman MW, Luo F, Cheng H, Zhao JJ and Liu P: Chemopreventive effects of aspirin at a glance. Biochim Biophys Acta. 1855:254–263. 2015.PubMed/NCBI View Article : Google Scholar

9 

Yang G, Wang Y, Feng J, Liu Y, Wang T, Zhao M, Ye L and Zhang X: Aspirin suppresses the abnormal lipid metabolism in liver cancer cells via disrupting an NFκB-ACSL1 signaling. Biochem Biophys Res Commun. 486:827–832. 2017.PubMed/NCBI View Article : Google Scholar

10 

Liu J, Wei X, Wu Y, Wang Y, Qiu Y, Shi J, Zhou H, Lu Z, Shao M, Yu L, et al: Giganteaside D induces ROS-mediated apoptosis in human hepatocellular carcinoma cells through the MAPK pathway. Cell Oncol (Dordr). 39:333–342. 2016.PubMed/NCBI View Article : Google Scholar

11 

Sun Y, Liu WZ, Liu T, Feng X, Yang N and Zhou HF: Signaling pathway of MAPK/ERK in cell proliferation, differentiation, migration, senescence and apoptosis. J Recept Signal Transduct Res. 35:600–604. 2015.PubMed/NCBI View Article : Google Scholar

12 

Liang Z, Chi YJ, Lin GQ, Luo SH, Jiang QY and Chen YK: MiRNA-26a promotes angiogenesis in a rat model of cerebral infarction via PI3K/AKT and MAPK/ERK pathway. Eur Rev Med Pharmacol Sci. 22:3485–3492. 2018.PubMed/NCBI View Article : Google Scholar

13 

Lei YY, Wang WJ, Mei JH and Wang CL: Mitogen-activated protein kinase signal transduction in solid tumors. Asian Pac J Cancer Prev. 15:8539–8548. 2014.PubMed/NCBI View Article : Google Scholar

14 

Kim EK and Choi EJ: Compromised MAPK signaling in human diseases: An update. Arch Toxicol. 89:867–882. 2015.PubMed/NCBI View Article : Google Scholar

15 

Geest CR and Coffer PJ: MAPK signaling pathways in the regulation of hematopoiesis. J Leukoc Biol. 86:237–250. 2009.PubMed/NCBI View Article : Google Scholar

16 

Liu ZJ, Xiao M, Balint K, Smalley KS, Brafford P, Qiu R, Pinnix CC, Li X and Herlyn M: Notch1 signaling promotes primary melanoma progression by activating mitogen-activated protein kinase/phosphatidylinositol 3-kinase-Akt pathways and up-regulating N-cadherin expression. Cancer Res. 66:4182–4190. 2006.PubMed/NCBI View Article : Google Scholar

17 

Rehman SK, Li SH, Wyszomierski SL, Wang Q, Li P, Sahin O, Xiao Y, Zhang S, Xiong Y, Yang J, et al: 14-3-3ζ orchestrates mammary tumor onset and progression via miR-221-mediated cell proliferation. Cancer Res. 74:363–373. 2014.PubMed/NCBI View Article : Google Scholar

18 

Tibbles LA and Woodgett JR: The stress-activated protein kinase pathways. Cell Mol Life Sci. 55:1230–1254. 1999.PubMed/NCBI View Article : Google Scholar

19 

Zhang W and Liu HT: MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell Res. 12:9–18. 2002.PubMed/NCBI View Article : Google Scholar

20 

Ryu MJ and Chung HS: [10]-Gingerol induces mitochondrial apoptosis through activation of MAPK pathway in HCT116 human colon cancer cells. In Vitro Cell Dev Biol Anim. 51:92–101. 2015.PubMed/NCBI View Article : Google Scholar

21 

Wu T, Ren MX, Chen GP, Jin ZM and Wang G: Rrp15 affects cell cycle, proliferation, and apoptosis in NIH3T3 cells. FEBS Open Bio. 6:1085–1092. 2016.PubMed/NCBI View Article : Google Scholar

22 

Estaquier J, Vallette F, Vayssiere JL and Mignotte B: The mitochondrial pathways of apoptosis. Adv Exp Med Biol. 942:157–183. 2012.PubMed/NCBI View Article : Google Scholar

23 

Ryu MJ, Kim AD, Kang KA, Chung HS, Kim HS, Suh IS, Chang WY and Hyun JW: The green algae Ulva fasciata Delile extract induces apoptotic cell death in human colon cancer cells. In Vitro Cell Dev Biol Anim. 49:74–81. 2013.PubMed/NCBI View Article : Google Scholar

24 

Stadheim TA and Kucera GL: c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) is required for mitoxantrone- and anisomycin-induced apoptosis in HL-60 cells. Leuk Res. 26:55–65. 2002.PubMed/NCBI View Article : Google Scholar

25 

Sarbacker GB, Lusk KA, Flieller LA and Van Liew JR: Aspirin use for the primary prevention of cardiovascular disease in the elderly. Consult Pharm. 31:24–32. 2016.PubMed/NCBI View Article : Google Scholar

26 

Pasche B, Wang M, Pennison M and Jimenez H: Prevention and treatment of cancer with aspirin: Where do we stand? Semin Oncol. 41:397–401. 2014.PubMed/NCBI View Article : Google Scholar

27 

Goldar S, Khaniani MS, Derakhshan SM and Baradaran B: Molecular mechanisms of apoptosis and roles in cancer development and treatment. Asian Pac J Cancer Prev. 16:2129–2144. 2015.PubMed/NCBI View Article : Google Scholar

28 

Suvarna V, Singh V and Murahari M: Current overview on the clinical update of Bcl-2 anti-apoptotic inhibitors for cancer therapy. Eur J Pharmacol. 862(172655)2019.PubMed/NCBI View Article : Google Scholar

29 

Wang X: The expanding role of mitochondria in apoptosis. Genes Dev. 15:2922–2933. 2001.PubMed/NCBI

30 

Peña-Blanco A and García-Sáez AJ: Bax, Bak and beyond - mitochondrial performance in apoptosis. FEBS J. 285:416–431. 2018.PubMed/NCBI View Article : Google Scholar

31 

Nagappan A, Park KI, Park HS, Kim JA, Hong GE, Kang SR, Lee DH, Kim EH, Lee WS, Won CK, et al: Vitamin C induces apoptosis in AGS cells by down-regulation of 14-3-3σ via a mitochondrial dependent pathway. Food Chem. 135:1920–1928. 2012.PubMed/NCBI View Article : Google Scholar

32 

Leung HW, Yang WH, Lai MY, Lin CJ and Lee HZ: Inhibition of 12-lipoxygenase during baicalein-induced human lung nonsmall carcinoma H460 cell apoptosis. Food Chem Toxicol. 45:403–411. 2007.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Qian Y, Dai H and Li H: Low‑doses of aspirin promote the growth of human PC‑9 lung cancer cells through activation of the MAPK family. Exp Ther Med 22: 1440, 2021.
APA
Qian, Y., Dai, H., & Li, H. (2021). Low‑doses of aspirin promote the growth of human PC‑9 lung cancer cells through activation of the MAPK family. Experimental and Therapeutic Medicine, 22, 1440. https://doi.org/10.3892/etm.2021.10875
MLA
Qian, Y., Dai, H., Li, H."Low‑doses of aspirin promote the growth of human PC‑9 lung cancer cells through activation of the MAPK family". Experimental and Therapeutic Medicine 22.6 (2021): 1440.
Chicago
Qian, Y., Dai, H., Li, H."Low‑doses of aspirin promote the growth of human PC‑9 lung cancer cells through activation of the MAPK family". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1440. https://doi.org/10.3892/etm.2021.10875
Copy and paste a formatted citation
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Spandidos Publications style
Qian Y, Dai H and Li H: Low‑doses of aspirin promote the growth of human PC‑9 lung cancer cells through activation of the MAPK family. Exp Ther Med 22: 1440, 2021.
APA
Qian, Y., Dai, H., & Li, H. (2021). Low‑doses of aspirin promote the growth of human PC‑9 lung cancer cells through activation of the MAPK family. Experimental and Therapeutic Medicine, 22, 1440. https://doi.org/10.3892/etm.2021.10875
MLA
Qian, Y., Dai, H., Li, H."Low‑doses of aspirin promote the growth of human PC‑9 lung cancer cells through activation of the MAPK family". Experimental and Therapeutic Medicine 22.6 (2021): 1440.
Chicago
Qian, Y., Dai, H., Li, H."Low‑doses of aspirin promote the growth of human PC‑9 lung cancer cells through activation of the MAPK family". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1440. https://doi.org/10.3892/etm.2021.10875
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