Open Access

Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H2O2‑induced oxidative damage and apoptosis in vitro

  • Authors:
    • Fang-Mei Zhou
    • Jing-Jing Huang
    • Xu-Jiao Hu
    • Jingwei Wang
    • Bing-Qi Zhu
    • Zhi-Shan Ding
    • Shigao Huang
    • Jing-Jing Fang
  • View Affiliations

  • Published online on: October 13, 2021     https://doi.org/10.3892/etm.2021.10878
  • Article Number: 1443
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Hydrogen peroxide (H2O2) can induce apoptosis by releasing reactive oxygen species (ROS) and reactive nitrogen species, which cause mitochondrial damage. The present study aimed to investigate the protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H2O2‑induced oxidative damage and apoptosis in vitro. The bioactivity of total flavonoids (TFs) and five monomeric flavonoids [cardamonin (Car), pinostrobin chalcone, wogonin, chrysin and pinocembrin] from the leaves of Carya cathayensis Sarg. (LCCS) were tested to prevent oxidative damage to rat aortic endothelial cells (RAECs) induced by H2O2. Oxidated superoxide dismutase, glutathione peroxidase, malondialdehyde, lactate dehydrogenase and ROS were analyzed to evaluate the antioxidant activity. Gene and protein expression patterns were assessed using reverse transcription‑quantitative PCR and western blotting, respectively. The results indicated that TFs and Car inhibited H2O2‑induced cytotoxicity and apoptosis of RAECs. Additionally, they regulated the level of oxidase and inhibited the production of ROS. Overall, the TFs extracted from LCCS could potentially be developed as effective candidate drugs to prevent oxidative stress in the future; moreover, they could also provide a direction in investigations for preventing antioxidant activity through the ROS pathway.

Related Articles

Journal Cover

December-2021
Volume 22 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhou F, Huang J, Hu X, Wang J, Zhu B, Ding Z, Huang S and Fang J: Protective effects of flavonoids from the leaves of <em>Carya cathayensis</em> Sarg. against H<sub>2</sub>O<sub>2</sub>‑induced oxidative damage and apoptosis <em>in vitro</em>. Exp Ther Med 22: 1443, 2021
APA
Zhou, F., Huang, J., Hu, X., Wang, J., Zhu, B., Ding, Z. ... Fang, J. (2021). Protective effects of flavonoids from the leaves of <em>Carya cathayensis</em> Sarg. against H<sub>2</sub>O<sub>2</sub>‑induced oxidative damage and apoptosis <em>in vitro</em>. Experimental and Therapeutic Medicine, 22, 1443. https://doi.org/10.3892/etm.2021.10878
MLA
Zhou, F., Huang, J., Hu, X., Wang, J., Zhu, B., Ding, Z., Huang, S., Fang, J."Protective effects of flavonoids from the leaves of <em>Carya cathayensis</em> Sarg. against H<sub>2</sub>O<sub>2</sub>‑induced oxidative damage and apoptosis <em>in vitro</em>". Experimental and Therapeutic Medicine 22.6 (2021): 1443.
Chicago
Zhou, F., Huang, J., Hu, X., Wang, J., Zhu, B., Ding, Z., Huang, S., Fang, J."Protective effects of flavonoids from the leaves of <em>Carya cathayensis</em> Sarg. against H<sub>2</sub>O<sub>2</sub>‑induced oxidative damage and apoptosis <em>in vitro</em>". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1443. https://doi.org/10.3892/etm.2021.10878