Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H2O2‑induced oxidative damage and apoptosis in vitro

Zhou,Fang-Mei; Huang,Jing-Jing; Hu,Xu-Jiao; Wang,Jingwei; Zhu,Bing-Qi; Ding,Zhi-Shan; Huang,Shigao; Fang,Jing-Jing

doi:10.3892/etm.2021.10878

Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H₂O₂‑induced oxidative damage and apoptosis in vitro

Authors:
- Fang-Mei Zhou
- Jing-Jing Huang
- Xu-Jiao Hu
- Jingwei Wang
- Bing-Qi Zhu
- Zhi-Shan Ding
- Shigao Huang
- Jing-Jing Fang
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Affiliations: Technology Teaching Center of Medical Laboratory and Quarantine, School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Inspection Department, Yinzhou People's Hospital, Ningbo, Zhejiang 315040, P.R. China, Department of Pathology, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Faculty of Health Sciences, University of Macau, Taipa 999078, Macau SAR, P.R. China
Published online on: October 13, 2021 https://doi.org/10.3892/etm.2021.10878
Article Number: 1443
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hydrogen peroxide (H₂O₂) can induce apoptosis by releasing reactive oxygen species (ROS) and reactive nitrogen species, which cause mitochondrial damage. The present study aimed to investigate the protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H₂O₂‑induced oxidative damage and apoptosis in vitro. The bioactivity of total flavonoids (TFs) and five monomeric flavonoids [cardamonin (Car), pinostrobin chalcone, wogonin, chrysin and pinocembrin] from the leaves of Carya cathayensis Sarg. (LCCS) were tested to prevent oxidative damage to rat aortic endothelial cells (RAECs) induced by H₂O₂. Oxidated superoxide dismutase, glutathione peroxidase, malondialdehyde, lactate dehydrogenase and ROS were analyzed to evaluate the antioxidant activity. Gene and protein expression patterns were assessed using reverse transcription‑quantitative PCR and western blotting, respectively. The results indicated that TFs and Car inhibited H₂O₂‑induced cytotoxicity and apoptosis of RAECs. Additionally, they regulated the level of oxidase and inhibited the production of ROS. Overall, the TFs extracted from LCCS could potentially be developed as effective candidate drugs to prevent oxidative stress in the future; moreover, they could also provide a direction in investigations for preventing antioxidant activity through the ROS pathway.

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