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Comprehensive analysis of mRNAs, lncRNAs and circRNAs in the early phase of microglial activation

  • Authors:
    • Jiagen Wen
    • Yujie Liu
    • Zhen Zhan
    • Shiqing Chen
    • Bingfeng Hu
    • Jinfang Ge
    • Qiliang Xie
  • View Affiliations / Copyright

    Affiliations: Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, Hefei, Anhui 230051, P.R. China, Department of Gynecology and Obstetrics, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, P.R. China, Department of Pediatrics, Children's Hospital of Anhui Medical University, Hefei, Anhui 230051, P.R. China
    Copyright: © Wen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1460
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    Published online on: October 20, 2021
       https://doi.org/10.3892/etm.2021.10895
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Abstract

Sepsis‑associated encephalopathy (SAE) is a common complication of sepsis that may seriously affect the prognosis and quality of life of patients with sepsis. Microglial activation is vital to the neuroinflammation and the pathology of SAE. In the present study, in vitro cultured BV‑2 microglial cells stimulated with lipopolysaccharide (LPS) were employed as a model of microglia activation. The altered profiles of long noncoding (lnc)RNAs, circular (circ)RNAs and mRNAs in BV‑2 cells after 4 h of LPS exposure were arrayed by using the Agilent competing endogenous (ce)RNA Microarray Chip. Using fold change >2 and P<0.05 as the cutoff criteria, 1,135 mRNAs and 2,488 lncRNAs were determined to be upregulated and 630 mRNAs and 744 lncRNAs to be downregulated. The number of differentially expressed circRNAs was lower, with 140 upregulated and 123 downregulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of DE mRNAs suggested that inflammatory responses, as well as lipid metabolism, were involved in microglial activation. Furthermore, analyses of ceRNA networks of the lncRNA‑miRNA‑mRNA or circRNA‑miRNA‑mRNA interrelations were performed. The present study revealed a multitude of novel candidate mRNAs, lncRNAs and circRNAs involved in microglial activation, which may improve the current knowledge on neuroinflammation and provide potential therapeutic targets for SAE.
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Spandidos Publications style
Wen J, Liu Y, Zhan Z, Chen S, Hu B, Ge J and Xie Q: Comprehensive analysis of mRNAs, lncRNAs and circRNAs in the early phase of microglial activation. Exp Ther Med 22: 1460, 2021.
APA
Wen, J., Liu, Y., Zhan, Z., Chen, S., Hu, B., Ge, J., & Xie, Q. (2021). Comprehensive analysis of mRNAs, lncRNAs and circRNAs in the early phase of microglial activation. Experimental and Therapeutic Medicine, 22, 1460. https://doi.org/10.3892/etm.2021.10895
MLA
Wen, J., Liu, Y., Zhan, Z., Chen, S., Hu, B., Ge, J., Xie, Q."Comprehensive analysis of mRNAs, lncRNAs and circRNAs in the early phase of microglial activation". Experimental and Therapeutic Medicine 22.6 (2021): 1460.
Chicago
Wen, J., Liu, Y., Zhan, Z., Chen, S., Hu, B., Ge, J., Xie, Q."Comprehensive analysis of mRNAs, lncRNAs and circRNAs in the early phase of microglial activation". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1460. https://doi.org/10.3892/etm.2021.10895
Copy and paste a formatted citation
x
Spandidos Publications style
Wen J, Liu Y, Zhan Z, Chen S, Hu B, Ge J and Xie Q: Comprehensive analysis of mRNAs, lncRNAs and circRNAs in the early phase of microglial activation. Exp Ther Med 22: 1460, 2021.
APA
Wen, J., Liu, Y., Zhan, Z., Chen, S., Hu, B., Ge, J., & Xie, Q. (2021). Comprehensive analysis of mRNAs, lncRNAs and circRNAs in the early phase of microglial activation. Experimental and Therapeutic Medicine, 22, 1460. https://doi.org/10.3892/etm.2021.10895
MLA
Wen, J., Liu, Y., Zhan, Z., Chen, S., Hu, B., Ge, J., Xie, Q."Comprehensive analysis of mRNAs, lncRNAs and circRNAs in the early phase of microglial activation". Experimental and Therapeutic Medicine 22.6 (2021): 1460.
Chicago
Wen, J., Liu, Y., Zhan, Z., Chen, S., Hu, B., Ge, J., Xie, Q."Comprehensive analysis of mRNAs, lncRNAs and circRNAs in the early phase of microglial activation". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1460. https://doi.org/10.3892/etm.2021.10895
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