Open Access

miR‑199b‑5p mediates adriamycin‑induced podocyte apoptosis by inhibiting the expression of RGS10

  • Authors:
    • Gaoting Qu
    • Tiantian He
    • Aisuo Dai
    • Yajie Zhao
    • Dian Guan
    • Shanwen Li
    • Huimin Shi
    • Weihua Gan
    • Aiqing Zhang
  • View Affiliations

  • Published online on: October 21, 2021     https://doi.org/10.3892/etm.2021.10904
  • Article Number: 1469
  • Copyright: © Qu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Podocyte apoptosis is a key risk factor for the progression of kidney diseases. MicroRNA (miR)‑199b‑5p has been shown to be involved in cell apoptosis. However, the molecular mechanisms of miR‑199b‑5p in podocyte apoptosis remain uncertain. Thus, the present study aimed to investigate whether miR‑199b‑5p participates in the regulation of podocyte apoptosis and to elucidate the involved mechanisms of this process. A podocyte apoptosis model was constructed using adriamycin (ADR) in vitro. miR‑199b‑5p mimic and inhibitor were transfected in podocytes to change the expression level of miR‑199b‑5p. RNA expression was examined by reverse transcription‑quantitative PCR. Western blotting was used to measure protein expression. Apoptosis was monitored via flow cytometry and detection of apoptosis‑associated proteins. The results from the present study demonstrated that miR‑199b‑5p was upregulated and that regulator of G‑protein signaling 10 (RGS10) was downregulated in ADR‑stimulated podocytes. Overexpression of miR‑199b‑5p could inhibit RGS10 expression and stimulate podocyte apoptosis, whereas miR‑199b‑5p knockdown restored the levels of RGS10 and ameliorated podocyte apoptosis in ADR‑induced podocytes. Furthermore, the effects of miR‑199b‑5p overexpression could be significantly reversed by RGS10 overexpression. In addition, podocyte transfection of miR‑199b‑5p activated the AKT/mechanistic target of rapamycin (mTOR) signaling, which was blocked following RGS10 overexpression. Taken together, the present study demonstrated that miR‑199b‑5p upregulation could promote podocyte apoptosis by inhibiting the expression of RGS10 through the activation of AKT/mTOR signaling.
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December-2021
Volume 22 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Qu G, He T, Dai A, Zhao Y, Guan D, Li S, Shi H, Gan W and Zhang A: miR‑199b‑5p mediates adriamycin‑induced podocyte apoptosis by inhibiting the expression of RGS10. Exp Ther Med 22: 1469, 2021
APA
Qu, G., He, T., Dai, A., Zhao, Y., Guan, D., Li, S. ... Zhang, A. (2021). miR‑199b‑5p mediates adriamycin‑induced podocyte apoptosis by inhibiting the expression of RGS10. Experimental and Therapeutic Medicine, 22, 1469. https://doi.org/10.3892/etm.2021.10904
MLA
Qu, G., He, T., Dai, A., Zhao, Y., Guan, D., Li, S., Shi, H., Gan, W., Zhang, A."miR‑199b‑5p mediates adriamycin‑induced podocyte apoptosis by inhibiting the expression of RGS10". Experimental and Therapeutic Medicine 22.6 (2021): 1469.
Chicago
Qu, G., He, T., Dai, A., Zhao, Y., Guan, D., Li, S., Shi, H., Gan, W., Zhang, A."miR‑199b‑5p mediates adriamycin‑induced podocyte apoptosis by inhibiting the expression of RGS10". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1469. https://doi.org/10.3892/etm.2021.10904