Tea polyphenols alleviate hydrogen peroxide‑induced oxidative stress damage through the Mst/Nrf2 axis and the Keap1/Nrf2/HO‑1 pathway in murine RAW264.7 cells

Li,Qian; Qiu,Zhaoyan; Wang,Yan; Guo,Chunyan; Cai,Xu; Zhang,Yandong; Liu,Li; Xue,Hongkun; Tang,Jintian

doi:10.3892/etm.2021.10908

Tea polyphenols alleviate hydrogen peroxide‑induced oxidative stress damage through the Mst/Nrf2 axis and the Keap1/Nrf2/HO‑1 pathway in murine RAW264.7 cells

Authors:
- Qian Li
- Zhaoyan Qiu
- Yan Wang
- Chunyan Guo
- Xu Cai
- Yandong Zhang
- Li Liu
- Hongkun Xue
- Jintian Tang
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Affiliations: Key Laboratory of Particle and Radiation Imaging, Ministry of Education, Department of Engineering Physics, Tsinghua University, Beijing 100084, P.R. China, Department of General Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, P.R. China, State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, Shanghai 200437, P.R. China, Department of Pharmacy, Hebei North University, Zhangjiakou, Hebei 075000, P.R. China
Published online on: October 22, 2021 https://doi.org/10.3892/etm.2021.10908
Article Number: 1473
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tea polyphenols (TPs) are the major bioactive extract from green tea that have been extensively reported to prevent and treat oxidative stress damage. In previous studies, TPs have been demonstrated to protect cells against oxidative injury induced by hydrogen peroxide (H₂O₂). However, the underlying mechanism remains unclear. The aim of the current study was to investigate whether the protective and regulatory effects of TPs on oxidative stress damage were dependent on the mammalian STE20‑like protein kinase (Mst)/nuclear factor (erythroid‑derived 2)‑like 2 (Nrf2) axis and the Kelch‑like ECH‑associated protein 1 (Keap1)/Nrf2/heme oxygenase 1 (HO‑1) pathway in RAW264.7 cells, a murine macrophage cell line. Maintaining a certain range of intracellular reactive oxygen species (ROS) levels is critical to basic cellular activities, while excessive ROS generation can override the antioxidant capacity of the cell and result in oxidative stress damage. The inhibition of ROS generation offers an effective target for preventing oxidative damage. The results of the present study revealed that pretreatment with TPs inhibited the production of intracellular ROS and protected RAW264.7 cells from H₂O₂‑induced oxidative damage. TPs was also demonstrated to attenuate the production of nitric oxide and malondialdehyde and increase the levels of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase). In addition, following TPs treatment, alterations in Mst1/2 at the mRNA and protein level inhibited the production of ROS and promoted the self‑regulation of antioxidation. TPs‑induced Keap1 gene downregulation also increased the expression of Nrf2 and HO‑1. Collectively, the results of the present study demonstrated that TPs provided protection against H2O2‑induced oxidative injury in RAW264.7 cells.

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