Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
January-2022 Volume 23 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2022 Volume 23 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Porphyromonas gingivalis lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1, cyclin A and cyclin B1

  • Authors:
    • Jiajing Lu
    • Yajing Hu
    • Zhongyuan Tang
    • Chengfei Zhang
    • Lijian Jin
    • Min Gu
    • Yanqi Yang
  • View Affiliations / Copyright

    Affiliations: Division of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR 999077, P.R. China, Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR 999077, P.R. China, Division of Periodontology and Implant Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR 999077, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 2
    |
    Published online on: October 26, 2021
       https://doi.org/10.3892/etm.2021.10925
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Human periodontal ligament cells (hPDLCs) play a notable role in periodontal tissue homeostasis and regeneration. However, the effect of Porphyromonas gingivalis lipopolysaccharide (Pg‑LPS) on the proliferation of hPDLCs remains unclear. The present study investigated the effects of Pg‑LPS on the proliferation profile of hPDLCs, and the involvement of cyclins and cyclin‑dependent kinases in the process. hPDLCs were treated with Pg‑LPS, and cell proliferation and cycle were detected using Cell Counting Kit‑8 assays and flow cytometry. The mRNA expression levels of the cyclins and cyclin‑dependent kinases (CDKs), including cyclins A, B1, D1 and D2 and CDK1, 2 and 4, were detected using reverse transcription‑quantitative PCR. The protein expression levels of cyclins A, B1 and D1 were analysed using western blotting. The proliferation of hPDLCs was significantly increased after treatment with Pg‑LPS at the concentrations of 0.001, 0.01, 0.1, 1 and 10 µg/ml for 24, 36 and 48 h compared with the cells cultured without LPS (P<0.01). The proliferation index of hPDLCs was significantly enhanced after treatment with Pg‑LPS (0.0001, 0.001, 0.01, 0.1, 1 and 10 µg/ml) for 24 h (P<0.01). However, the S‑phase fraction (SPF) only significantly increased after treatment with Pg‑LPS at 0.01 µg/ml for 24 h (P<0.05), while the G2/M‑phase fraction increased (P<0.01) and the G0/G1‑phase fraction decreased (P<0.01) compared with the controls. The proliferation index and SPF increased, peaked at 24 h and then decreased at 48 h in both Pg‑LPS‑stimulated and control groups. Notably, Pg‑LPS significantly upregulated the expression levels of cyclins D1, A and B1 after 24 h compared with those in the controls. Overall, the present study indicated that Pg‑LPS may enhance the proliferation of hPDLCs, potentially through upregulation of cyclins D1, A and B1.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Garant PR: Collagen resorption by fibroblasts. A theory of fibroblastic maintenance of the periodontal ligament. J Periodontol. 47:380–390. 1976.PubMed/NCBI View Article : Google Scholar

2 

Hughes FJ, Ghuman M and Talal A: Periodontal regeneration: A challenge for the tissue engineer? Proc Inst Mech Eng H. 224:1345–1358. 2010.PubMed/NCBI View Article : Google Scholar

3 

Lekic P and McCulloch C: Periodontal ligament cell populations: The central role of fibroblasts in creating a unique tissue. Anat Rec. 245:327–341. 1996.PubMed/NCBI View Article : Google Scholar

4 

McCulloch CA and Melcher AH: Continuous labelling of the periodontal ligament of mice. J Periodontal Res. 18:231–241. 1983.PubMed/NCBI View Article : Google Scholar

5 

Liu J, Tang X, Li C, Pan C, Li Q, Geng F and Pan Y: Porphyromonas gingivalis promotes the cell cycle and inflammatory cytokine production in periodontal ligament fibroblasts. Arch Oral Biol. 60:1153–1161. 2015.PubMed/NCBI View Article : Google Scholar

6 

Mabuchi R, Matsuzaka K and Shimono M: Cell proliferation and cell death in periodontal ligaments during orthodontic tooth movement. J Periodontal Res. 37:118–124. 2002.PubMed/NCBI View Article : Google Scholar

7 

Sherr CJ: G1 phase progression: Cycling on cue. Cell. 79:551–555. 1994.PubMed/NCBI View Article : Google Scholar

8 

Girard F, Strausfeld U, Fernandez A and Lamb NJ: Cyclin A is required for the onset of DNA replication in mammalian fibroblasts. Cell. 67:1169–1179. 1991.PubMed/NCBI View Article : Google Scholar

9 

Sweeney KJ, Sarcevic B, Sutherland RL and Musgrove EA: Cyclin D2 activates Cdk2 in preference to Cdk4 in human breast epithelial cells. Oncogene. 14:1329–1340. 1997.PubMed/NCBI View Article : Google Scholar

10 

Takizawa CG and Morgan DO: Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C. Curr Opin Cell Biol. 12:658–665. 2000.PubMed/NCBI View Article : Google Scholar

11 

Kato H, Taguchi Y, Tominaga K, Umeda M and Tanaka A: Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. Arch Oral Biol. 59:167–175. 2014.PubMed/NCBI View Article : Google Scholar

12 

Morandini AC, Sipert CR, Gasparoto TH, Greghi SL, Passanezi E, Rezende ML, Sant'ana AP, Campanelli AP, Garlet GP and Santos CF: Differential production of macrophage inflammatory protein-1alpha, stromal-derived factor-1, and IL-6 by human cultured periodontal ligament and gingival fibroblasts challenged with lipopolysaccharide from P. gingivalis. J Periodontol. 81:310–317. 2010.PubMed/NCBI View Article : Google Scholar

13 

Pathirana RD, O'Brien-Simpson NM and Reynolds EC: Host immune responses to Porphyromonas gingivalis antigens. Periodontol 2000. 52:218–237. 2010.PubMed/NCBI View Article : Google Scholar

14 

Wada N, Maeda H, Yoshimine Y and Akamine A: Lipopolysaccharide stimulates expression of osteoprotegerin and receptor activator of NF-kappa B ligand in periodontal ligament fibroblasts through the induction of interleukin-1 beta and tumor necrosis factor-alpha. Bone. 35:629–635. 2004.PubMed/NCBI View Article : Google Scholar

15 

Krajewski AC, Biessei J, Kunze M, Maersch S, Perabo L and Noack MJ: Influence of lipopolysaccharide and interleukin-6 on RANKL and OPG expression and release in human periodontal ligament cells. APMIS. 117:746–754. 2009.PubMed/NCBI View Article : Google Scholar

16 

Park YD, Kim YS, Jung YM, Lee SI, Lee YM, Bang JB and Kim EC: Porphyromonas gingivalis lipopolysaccharide regulates interleukin (IL)-17 and IL-23 expression via SIRT1 modulation in human periodontal ligament cells. Cytokine. 60:284–293. 2012.PubMed/NCBI View Article : Google Scholar

17 

Seo T, Cha S, Kim TI, Lee JS and Woo KM: Porphyromonas gingivalis-derived lipopolysaccharide-mediated activation of MAPK signaling regulates inflammatory response and differentiation in human periodontal ligament fibroblasts. J Microbiol. 50:311–319. 2012.PubMed/NCBI View Article : Google Scholar

18 

Yamaji Y, Kubota T, Sasaguri K, Sato S, Suzuki Y, Kumada H and Umemoto T: Inflammatory cytokine gene expression in human periodontal ligament fibroblasts stimulated with bacterial lipopolysaccharides. Infect Immun. 63:3576–3581. 1995.PubMed/NCBI View Article : Google Scholar

19 

Yu B, Li Q and Zhou M: LPS-induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions. Int J Mol Med. 43:2341–2351. 2019.PubMed/NCBI View Article : Google Scholar

20 

Takemura A, Matsuda N, Kimura S, Fujiwara T, Nakagawa I and Hamada S: Porphyromonas gingivalis lipopolysaccharide modulates the responsiveness of human periodontal ligament fibroblasts to platelet-derived growth factor. J Periodontal Res. 33:400–407. 1998.PubMed/NCBI View Article : Google Scholar

21 

Tong J, Sun D, Yang C, Wang Y, Sun S, Li Q, Bao J and Liu Y: Serum starvation and thymidine double blocking achieved efficient cell cycle synchronization and altered the expression of p27, p53, bcl-2 in canine breast cancer cells. Res Vet Sci. 105:10–14. 2016.PubMed/NCBI View Article : Google Scholar

22 

Khammanit R, Chantakru S, Kitiyanant Y and Saikhun J: Effect of serum starvation and chemical inhibitors on cell cycle synchronization of canine dermal fibroblasts. Theriogenology. 70:27–34. 2008.PubMed/NCBI View Article : Google Scholar

23 

Nishimura F, Terranova VP, Braithwaite M, Orman R, Ohyama H, Mineshiba J, Chou HH, Takashiba S and Murayama Y: Comparison of in vitro proliferative capacity of human periodontal ligament cells in juvenile and aged donors. Oral Dis. 3:162–166. 1997.PubMed/NCBI View Article : Google Scholar

24 

Liu F, Wu BL, Gao J, Huang X, Ma DD and Chen T: Effects of serum starvation on cell cycle synchronization in human dental pulp cells. Chin J Conserv Dent. 21:67–71. 2011.

25 

Mikami K, Haseba T and Ohno Y: Ethanol induces transient arrest of cell division (G2 + M block) followed by G0/G1 block: Dose effects of short- and longer-term ethanol exposure on cell cycle and cell functions. Alcohol Alcohol. 32:145–152. 1997.PubMed/NCBI View Article : Google Scholar

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

27 

Jönsson D, Nebel D, Bratthall G and Nilsson BO: LPS-induced MCP-1 and IL-6 production is not reversed by oestrogen in human periodontal ligament cells. Arch Oral Biol. 53:896–902. 2008.PubMed/NCBI View Article : Google Scholar

28 

Huang M, Yan F, Yao L, Li D, Zheng Y, Li Y and Lin M: Effects of cyclosporine A and Porphyromonas gingivalis-lipopolysaccharide on proliferation of human periodontal ligament fibroblasts in vitro. Chin J Stomatol Res. 5:470–476. 2011.

29 

Jung IH, Lee DE, Yun JH, Cho AR, Kim CS, You YJ, Kim SJ and Choi SH: Anti-inflammatory effect of (-)-epigallocatechin-3-gallate on Porphyromonas gingivalis lipopolysaccharide-stimulated fibroblasts and stem cells derived from human periodontal ligament. J Periodontal Implant Sci. 42:185–195. 2012.PubMed/NCBI View Article : Google Scholar

30 

Zhang F, Wu Z, Wan L and Yuan N: Effects of lipopolysaccharides on proliferation and alkaline phosphatase activity of periodontal ligament cells. Chin J Conserv Dent. 13:27–29. 2003.

31 

Jönsson D, Nebel D, Bratthall G and Nilsson BO: The human periodontal ligament cell: A fibroblast-like cell acting as an immune cell. J Periodontal Res. 46:153–157. 2011.PubMed/NCBI View Article : Google Scholar

32 

Scheres N, Laine ML, de Vries TJ, Everts V and van Winkelhoff AJ: Gingival and periodontal ligament fibroblasts differ in their inflammatory response to viable Porphyromonas gingivalis. J Periodontal Res. 45:262–270. 2010.PubMed/NCBI View Article : Google Scholar

33 

Itaya T, Kagami H, Okada K, Yamawaki A, Narita Y, Inoue M, Sumita Y and Ueda M: Characteristic changes of periodontal ligament-derived cells during passage. J Periodontal Res. 44:425–433. 2009.PubMed/NCBI View Article : Google Scholar

34 

Jia L, Wen Y and Xu X: Effects of culture conditions in vitro on the biological characteristics of periodontal ligament stem cells. Int J Stomatol. 45:255–260. 2018.

35 

Tominaga H, Ishiyama M, Ohseto F, Sasamoto K, Hamamoto T, Suzuki K and Watanabe M: A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Anal Commun. 36:47–50. 1999.

36 

Failli A, Legitimo A, Orsini G, Castagna M, Spisni R, Miccoli P and Consolini R: Antiproliferative effects of 5-fluorouracil and oxaliplatin in colon cancer cell lines: Comparison of three different cytotoxicity assays. J Biol Regul Homeost Agents. 27:275–284. 2013.PubMed/NCBI

37 

Shiba H, Nakanishi K, Sakata M, Fujita T, Uchida Y and Kurihara H: Effects of ageing on proliferative ability, and the expressions of secreted protein, acidic and rich in cysteine (SPARC) and osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human periodontal ligament cells. Mech Ageing Dev. 117:69–77. 2000.PubMed/NCBI View Article : Google Scholar

38 

Masai H, Matsumoto S, You Z, Yoshizawa-Sugata N and Oda M: Eukaryotic chromosome DNA replication: Where, when, and how? Annu Rev Biochem. 79:89–130. 2010.PubMed/NCBI View Article : Google Scholar

39 

Schafer KA: The cell cycle: A review. Vet Pathol. 35:461–478. 1998.PubMed/NCBI View Article : Google Scholar

40 

Gray JW, Dolbeare F, Pallavicini MG, Beisker W and Waldman F: Cell cycle analysis using flow cytometry. Int J Radiat Biol Relat Stud Phys Chem Med. 49:237–255. 1986.PubMed/NCBI View Article : Google Scholar

41 

Malumbres M, Sotillo R, Santamaria D, Galan J, Cerezo A, Ortega S, Dubus P and Barbacid M: Mammalian cells cycle without the D-type cyclin-dependent kinases Cdk4 and Cdk6. Cell. 118:493–504. 2004.PubMed/NCBI View Article : Google Scholar

42 

Kozar K, Ciemerych MA, Rebel VI, Shigematsu H, Zagozdzon A, Sicinska E, Geng Y, Yu Q, Bhattacharya S, Bronson RT, et al: Mouse development and cell proliferation in the absence of D-cyclins. Cell. 118:477–491. 2004.PubMed/NCBI View Article : Google Scholar

43 

Satyanarayana A and Kaldis P: Mammalian cell-cycle regulation: Several Cdks, numerous cyclins and diverse compensatory mechanisms. Oncogene. 28:2925–2939. 2009.PubMed/NCBI View Article : Google Scholar

44 

Mehra A, Lee KH and Hatzimanikatis V: Insights into the relation between mRNA and protein expression patterns: I. Theoretical considerations. Biotechnol Bioeng. 84:822–833. 2003.PubMed/NCBI View Article : Google Scholar

45 

Gedeon T and Bokes P: Delayed protein synthesis reduces the correlation between mRNA and protein fluctuations. Biophys J. 103:377–385. 2012.PubMed/NCBI View Article : Google Scholar

46 

Du A, Zhao S, Wan L, Liu T, Peng Z, Zhou Z, Liao Z and Fang H: MicroRNA expression profile of human periodontal ligament cells under the influence of Porphyromonas gingivalis LPS. J Cell Mol Med. 20:1329–1338. 2016.PubMed/NCBI View Article : Google Scholar

47 

Han Y, Wang F, Shao L, Huang P and Xu Y: LncRNA TUG1 mediates lipopolysaccharide-induced proliferative inhibition and apoptosis of human periodontal ligament cells by sponging miR-132. Acta Biochim Biophys Sin (Shanghai). 51:1208–1215. 2019.PubMed/NCBI View Article : Google Scholar

48 

Francis M, Pandya M, Gopinathan G, Lyu H, Ma W, Foyle D, Nares S and Luan X: Histone methylation mechanisms modulate the inflammatory response of periodontal ligament progenitors. Stem Cells Dev. 28:1015–1025. 2019.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lu J, Hu Y, Tang Z, Zhang C, Jin L, Gu M and Yang Y: <em>Porphyromonas gingivalis</em> lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1, cyclin A and cyclin B1. Exp Ther Med 23: 2, 2022.
APA
Lu, J., Hu, Y., Tang, Z., Zhang, C., Jin, L., Gu, M., & Yang, Y. (2022). <em>Porphyromonas gingivalis</em> lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1, cyclin A and cyclin B1. Experimental and Therapeutic Medicine, 23, 2. https://doi.org/10.3892/etm.2021.10925
MLA
Lu, J., Hu, Y., Tang, Z., Zhang, C., Jin, L., Gu, M., Yang, Y."<em>Porphyromonas gingivalis</em> lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1, cyclin A and cyclin B1". Experimental and Therapeutic Medicine 23.1 (2022): 2.
Chicago
Lu, J., Hu, Y., Tang, Z., Zhang, C., Jin, L., Gu, M., Yang, Y."<em>Porphyromonas gingivalis</em> lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1, cyclin A and cyclin B1". Experimental and Therapeutic Medicine 23, no. 1 (2022): 2. https://doi.org/10.3892/etm.2021.10925
Copy and paste a formatted citation
x
Spandidos Publications style
Lu J, Hu Y, Tang Z, Zhang C, Jin L, Gu M and Yang Y: <em>Porphyromonas gingivalis</em> lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1, cyclin A and cyclin B1. Exp Ther Med 23: 2, 2022.
APA
Lu, J., Hu, Y., Tang, Z., Zhang, C., Jin, L., Gu, M., & Yang, Y. (2022). <em>Porphyromonas gingivalis</em> lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1, cyclin A and cyclin B1. Experimental and Therapeutic Medicine, 23, 2. https://doi.org/10.3892/etm.2021.10925
MLA
Lu, J., Hu, Y., Tang, Z., Zhang, C., Jin, L., Gu, M., Yang, Y."<em>Porphyromonas gingivalis</em> lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1, cyclin A and cyclin B1". Experimental and Therapeutic Medicine 23.1 (2022): 2.
Chicago
Lu, J., Hu, Y., Tang, Z., Zhang, C., Jin, L., Gu, M., Yang, Y."<em>Porphyromonas gingivalis</em> lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1, cyclin A and cyclin B1". Experimental and Therapeutic Medicine 23, no. 1 (2022): 2. https://doi.org/10.3892/etm.2021.10925
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team