Influence of atherosclerosis on the molecular expression of the TRPC1/BK signal complex in the aortic smooth muscles of mice

Wang,Lian-Fa; Ling,Dong-Yun; Huang,Meng-Xun; Tao,Li-Wei; Tong,Quan-Xiu; Hou,Yong; Li,Hua; Chen,Zhen; Zhang,Bang-Zhu; Lu,Hong-Tao; Wang,Yun-Fei; Zhang,Xian-Ge

doi:10.3892/etm.2021.10926

Influence of atherosclerosis on the molecular expression of the TRPC1/BK signal complex in the aortic smooth muscles of mice

Authors:
- Lian-Fa Wang
- Dong-Yun Ling
- Meng-Xun Huang
- Li-Wei Tao
- Quan-Xiu Tong
- Yong Hou
- Hua Li
- Zhen Chen
- Bang-Zhu Zhang
- Hong-Tao Lu
- Yun-Fei Wang
- Xian-Ge Zhang
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Affiliations: Department of Cardiology, The 901st Hospital of Joint Logistics Support Force of PLA, Hefei, Anhui 230031, P.R. China, Department of Cardiology, The Second People's Hospital of Hefei City, Hefei, Anhui 230011, P.R. China, Department of Cardiothoracic Surgery, The Second People's Hospital of Fuyang City, Fuyang, Anhui 236000, P.R. China, Institute of Public Health and Nursing Research, Department of Healthcare Management, University of Bremen, 28359 Bremen, Germany
Published online on: October 26, 2021 https://doi.org/10.3892/etm.2021.10926
Article Number: 4
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Atherosclerosis (AS) is one a disease that seriously endangers human health. Previous studies have demonstrated that transient receptor potential channel‑1 (TRPC1)/large conductance Ca²⁺ activated K⁺ channel (BK) signal complex is widely distributed in arteries. Therefore, it was hypothesized that TRPC1‑BK signal complex may be a new target for the treatment of AS‑related diseases. Apolipoprotein E^‑/‑ (ApoE^‑/‑) mice were used to establish an atherosclerotic animal model in the present study, and the association between AS and the TRPC1‑BK signal complex was examined. The present study aimed to compare the differences in the expression levels of mRNAs and proteins of the TRPC1‑BK signal complex expressed in the aortic vascular smooth muscle tissue, between mice with AS and control mice. There were 10 mice in each group. Reverse transcription PCR, western blotting and immunohistochemistry were used to detect the differences in the mRNA and protein expression levels of TRPC1, BKα (the α subunit of BK) and BKβ₁ (the β₁ subunit of BK). The mRNA expression level of TRPC1 in AS model mice was significantly higher compared with that in the control group (P<0.05). However, the mRNA expression levels of BKα and BKβ1 were lower compared with those in the controls (both P<0.01). The mice in the ApoE^‑/‑ group successfully developed AS. In this group, the protein expression level of TRPC1 was significantly higher than that in the control group (P<0.01), while the protein expression levels of BKα and BKβ₁ were lower compared with those in the control group (P<0.01 and P<0.05, respectively). Collectively, it was identified that the protein and mRNA expression levels of the TRPC1/BK signal complex in the aortic vascular smooth muscle tissue could be influenced by the development of AS in mice. Hence, the TRPC1/BK signal complex may be a potential therapeutic target for the prevention and treatment of AS‑related complications in the future.

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