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Proteomic analysis of human umbilical cord serum exosomes using mass spectrometry and preliminary study of their biological activities in liver cancer cell lines

  • Authors:
    • Donglie Zhu
    • Wenhui Li
    • Cheng Fang
    • Ruozhe Yin
    • Mingzuo Jiang
    • Xing Lv
    • Yong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Gynecology and Obstetrics, Changhai Hospital, Shanghai 200438, P.R. China, Department of Hepatobiliary Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, P.R. China, Department of Emergency, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Gastroenterology and Hepatology, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, P.R. China, Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 44
    |
    Published online on: November 14, 2021
       https://doi.org/10.3892/etm.2021.10966
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Abstract

Exosomes are membranous extracellular vesicles 50‑100 nm in size, which are involved in cellular communication via the delivery of proteins, lipids and RNA. Emerging evidence shows that exosomes play a critical role in cancer. It has recently been revealed that maternal and umbilical cord serum (UCS)‑derived exosomes may enhance endothelial cell proliferation and migration. However, the role of exosomes isolated from the human umbilical cord in cancer development has not been investigated. To explore the potential differences in the composition and function of proteins from umbilical serum exosomes (UEs) and maternal serum exosomes, a proteomic analysis of exosomes was conducted using mass spectrometry and bioinformatics. Moreover, Cell Counting Kit‑8 assays and flow cytometry were used to study the biological effects of UEs on liver cancer cell lines. The present study demonstrated that UCS was enriched with proteins involved in extracellular matrix‑receptor interactions, which may be closely related to cell metastasis and proliferation. The findings further indicated that exosomes derived from human umbilical serum could inhibit the viability and induce apoptosis of liver cancer cells. This suggests that UCS‑derived exosomes may represent potential leads for the development of biotherapy for liver cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu D, Li W, Fang C, Yin R, Jiang M, Lv X and Chen Y: Proteomic analysis of human umbilical cord serum exosomes using mass spectrometry and preliminary study of their biological activities in liver cancer cell lines. Exp Ther Med 23: 44, 2022.
APA
Zhu, D., Li, W., Fang, C., Yin, R., Jiang, M., Lv, X., & Chen, Y. (2022). Proteomic analysis of human umbilical cord serum exosomes using mass spectrometry and preliminary study of their biological activities in liver cancer cell lines. Experimental and Therapeutic Medicine, 23, 44. https://doi.org/10.3892/etm.2021.10966
MLA
Zhu, D., Li, W., Fang, C., Yin, R., Jiang, M., Lv, X., Chen, Y."Proteomic analysis of human umbilical cord serum exosomes using mass spectrometry and preliminary study of their biological activities in liver cancer cell lines". Experimental and Therapeutic Medicine 23.1 (2022): 44.
Chicago
Zhu, D., Li, W., Fang, C., Yin, R., Jiang, M., Lv, X., Chen, Y."Proteomic analysis of human umbilical cord serum exosomes using mass spectrometry and preliminary study of their biological activities in liver cancer cell lines". Experimental and Therapeutic Medicine 23, no. 1 (2022): 44. https://doi.org/10.3892/etm.2021.10966
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu D, Li W, Fang C, Yin R, Jiang M, Lv X and Chen Y: Proteomic analysis of human umbilical cord serum exosomes using mass spectrometry and preliminary study of their biological activities in liver cancer cell lines. Exp Ther Med 23: 44, 2022.
APA
Zhu, D., Li, W., Fang, C., Yin, R., Jiang, M., Lv, X., & Chen, Y. (2022). Proteomic analysis of human umbilical cord serum exosomes using mass spectrometry and preliminary study of their biological activities in liver cancer cell lines. Experimental and Therapeutic Medicine, 23, 44. https://doi.org/10.3892/etm.2021.10966
MLA
Zhu, D., Li, W., Fang, C., Yin, R., Jiang, M., Lv, X., Chen, Y."Proteomic analysis of human umbilical cord serum exosomes using mass spectrometry and preliminary study of their biological activities in liver cancer cell lines". Experimental and Therapeutic Medicine 23.1 (2022): 44.
Chicago
Zhu, D., Li, W., Fang, C., Yin, R., Jiang, M., Lv, X., Chen, Y."Proteomic analysis of human umbilical cord serum exosomes using mass spectrometry and preliminary study of their biological activities in liver cancer cell lines". Experimental and Therapeutic Medicine 23, no. 1 (2022): 44. https://doi.org/10.3892/etm.2021.10966
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