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Salidroside promotes the osteogenic and odontogenic differentiation of human dental pulp stem cells through the BMP signaling pathway

  • Authors:
    • Xiaoling Wei
    • Jiayang Li
    • Hui Liu
    • Chenguang Niu
    • Dong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Endodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai 200001, P.R. China, Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Shanghai Stomatological Hospital, Fudan University, Shanghai 200001, P.R. China
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 55
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    Published online on: November 17, 2021
       https://doi.org/10.3892/etm.2021.10977
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Abstract

Regenerative endodontics, as an alternative approach, aims to regenerate dental pulp‑like tissues and is garnering the attention of clinical dentists. This is due to its reported biological benefits for dental therapeutics. Stem cells and their microenvironment serve an important role in the process of pulp regeneration. Regulation of the stem cell microenvironment and the directed differentiation of stem cells is becoming a topic of intensive research. Salidroside (SAL) is extracted from the root of Rhodiola rosea and it has been reported that SAL exerts antiaging, neuroprotective, hepatoprotective, cardioprotective and anticancer effects. However, the ability of SAL to regulate the osteo/odontogenic differentiation of hDPSCs remains to be elucidated. In the present study, the effect of SAL on the proliferation and osteogenic/odontogenic differentiation of human dental pulp stem cells (hDPSCs) was investigated. This was achieved by performing CCK‑8 ARS staining assay, reverse transcription‑quantitative PCR to detect mRNA of ALP, OSX, RUNX2, OCN, DSPP and BSP, western blotting to detect the protein of MAPK, Smad1/5/8, OSX, RUNX2, BSP and GAPDH and immunofluorescence assays to detect DSPP. The results indicated that SAL promoted the cell viability and the osteogenic/odontogenic differentiation of hDPSCs whilst increasing the expression of genes associated with osteogenic/odontogenic differentiation by ARS staining assay. In addition, SAL promoted osteogenic and odontogenic differentiation by activating the phosphorylation of Smad1/5/8. Collectively, these findings suggest that SAL promoted the osteogenic and odontogenic differentiation of hDPSCs activating the BMP signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wei X, Li J, Liu H, Niu C and Chen D: Salidroside promotes the osteogenic and odontogenic differentiation of human dental pulp stem cells through the BMP signaling pathway. Exp Ther Med 23: 55, 2022.
APA
Wei, X., Li, J., Liu, H., Niu, C., & Chen, D. (2022). Salidroside promotes the osteogenic and odontogenic differentiation of human dental pulp stem cells through the BMP signaling pathway. Experimental and Therapeutic Medicine, 23, 55. https://doi.org/10.3892/etm.2021.10977
MLA
Wei, X., Li, J., Liu, H., Niu, C., Chen, D."Salidroside promotes the osteogenic and odontogenic differentiation of human dental pulp stem cells through the BMP signaling pathway". Experimental and Therapeutic Medicine 23.1 (2022): 55.
Chicago
Wei, X., Li, J., Liu, H., Niu, C., Chen, D."Salidroside promotes the osteogenic and odontogenic differentiation of human dental pulp stem cells through the BMP signaling pathway". Experimental and Therapeutic Medicine 23, no. 1 (2022): 55. https://doi.org/10.3892/etm.2021.10977
Copy and paste a formatted citation
x
Spandidos Publications style
Wei X, Li J, Liu H, Niu C and Chen D: Salidroside promotes the osteogenic and odontogenic differentiation of human dental pulp stem cells through the BMP signaling pathway. Exp Ther Med 23: 55, 2022.
APA
Wei, X., Li, J., Liu, H., Niu, C., & Chen, D. (2022). Salidroside promotes the osteogenic and odontogenic differentiation of human dental pulp stem cells through the BMP signaling pathway. Experimental and Therapeutic Medicine, 23, 55. https://doi.org/10.3892/etm.2021.10977
MLA
Wei, X., Li, J., Liu, H., Niu, C., Chen, D."Salidroside promotes the osteogenic and odontogenic differentiation of human dental pulp stem cells through the BMP signaling pathway". Experimental and Therapeutic Medicine 23.1 (2022): 55.
Chicago
Wei, X., Li, J., Liu, H., Niu, C., Chen, D."Salidroside promotes the osteogenic and odontogenic differentiation of human dental pulp stem cells through the BMP signaling pathway". Experimental and Therapeutic Medicine 23, no. 1 (2022): 55. https://doi.org/10.3892/etm.2021.10977
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