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Identification of potential key genes and functional role of CENPF in osteosarcoma using bioinformatics and experimental analysis

  • Authors:
    • Yihui Ma
    • Jiaping Guo
    • Da Li
    • Xianhua Cai
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei 430070, P.R. China, Department of Orthopedics, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei 430070, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 80
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    Published online on: November 25, 2021
       https://doi.org/10.3892/etm.2021.11003
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Abstract

Osteosarcoma, which arises from bone tissue, is considered to be one of the most common types of cancer in children and teenagers. As the etiology of osteosarcoma has not been fully elucidated, the overall prognosis for patients is generally poor. In recent years, the development of bioinformatical technology has allowed researchers to identify numerous molecular biological characteristics associated with the prognosis of osteosarcoma using online databases. In the present study, Gene Expression Omnibus (GEO) database was used and three microarray datasets were obtained. The GEO2R web tool was utilized and differentially expressed genes (DEGs) in osteosarcoma tissue were identified. Venn analysis was performed to determine the intersection of the DEG profiles. DEGs were analyzed by Gene Ontology function and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Protein‑protein interactions (PPIs) between these DEGs were analyzed using the Search Tool for the Retrieval of Interacting Genes database, and the PPI network was then visualized using Cytoscape software. The top ten genes were identified based on measurement of degree, density of maximum neighborhood component, maximal clique centrality and mononuclear cell counts in the PPI network, and five overlapping genes [origin recognition complex subunit 6 (ORC6), IGF‑binding protein 5 (IGFBP5), minichromosome maintenance 10 replication initiation factor (MCM10), MET proto‑oncogene, receptor tyrosine kinase (MET) and centromere protein F (CENPF)] were identified. Additionally, three module networks were analyzed by Molecular Complex Detection (MCODE), and six key genes [ORC6, MCM10, DEP domain containing 1 (DEPDC1), CENPF, TIMELESS interacting protein (TIPIN) and shugoshin 1 (SGOL1)] were screened. Combined with the results from Cytoscape and MCODE, eight hub genes (ORC6, MCM10, DEPDC1, CENPF, TIPIN, SGOL1, MET and IGFBP5) were obtained. Furthermore, Kaplan‑Meier plotter survival analysis was used to evaluate the prognostic value of these eight hub genes in patients with osteosarcoma. Oncomine and GEPIA databases were applied to further confirm the expression levels of hub genes in tissue. Finally, the functional roles of the core gene CENPF were investigated using Cell Counting Kit‑8, wound healing and Transwell assays, which indicated that CENPF knockdown inhibited the proliferation, migration and invasion of osteosarcoma cells. These results provided potential prognostic markers, as well as a basis for further investigation of the mechanism underlying osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Guo J, Li D and Cai X: Identification of potential key genes and functional role of CENPF in osteosarcoma using bioinformatics and experimental analysis. Exp Ther Med 23: 80, 2022.
APA
Ma, Y., Guo, J., Li, D., & Cai, X. (2022). Identification of potential key genes and functional role of CENPF in osteosarcoma using bioinformatics and experimental analysis. Experimental and Therapeutic Medicine, 23, 80. https://doi.org/10.3892/etm.2021.11003
MLA
Ma, Y., Guo, J., Li, D., Cai, X."Identification of potential key genes and functional role of CENPF in osteosarcoma using bioinformatics and experimental analysis". Experimental and Therapeutic Medicine 23.1 (2022): 80.
Chicago
Ma, Y., Guo, J., Li, D., Cai, X."Identification of potential key genes and functional role of CENPF in osteosarcoma using bioinformatics and experimental analysis". Experimental and Therapeutic Medicine 23, no. 1 (2022): 80. https://doi.org/10.3892/etm.2021.11003
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Guo J, Li D and Cai X: Identification of potential key genes and functional role of CENPF in osteosarcoma using bioinformatics and experimental analysis. Exp Ther Med 23: 80, 2022.
APA
Ma, Y., Guo, J., Li, D., & Cai, X. (2022). Identification of potential key genes and functional role of CENPF in osteosarcoma using bioinformatics and experimental analysis. Experimental and Therapeutic Medicine, 23, 80. https://doi.org/10.3892/etm.2021.11003
MLA
Ma, Y., Guo, J., Li, D., Cai, X."Identification of potential key genes and functional role of CENPF in osteosarcoma using bioinformatics and experimental analysis". Experimental and Therapeutic Medicine 23.1 (2022): 80.
Chicago
Ma, Y., Guo, J., Li, D., Cai, X."Identification of potential key genes and functional role of CENPF in osteosarcoma using bioinformatics and experimental analysis". Experimental and Therapeutic Medicine 23, no. 1 (2022): 80. https://doi.org/10.3892/etm.2021.11003
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