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Article

GATA4 regulates osteogenic differentiation by targeting miR‑144‑3p

  • Authors:
    • Tao Qiu
    • Haotian Li
    • Tao Lu
    • Liping Shu
    • Chao Chen
    • Chunqing Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Trauma, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
  • Article Number: 83
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    Published online on: November 25, 2021
       https://doi.org/10.3892/etm.2021.11006
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Abstract

Numerous studies have demonstrated that microRNAs (miRNAs or miRs) play an important role in regulating osteogenic differentiation, but their specific regulatory mechanism requires further investigation. In the present study, it was revealed that during osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), the expression level of miR‑144‑3p was decreased with increased osteogenic induction duration and was negatively associated with osteogenic marker gene expression. Overexpression of miR‑144‑3p inhibited osteogenic differentiation, while inhibition of miR‑144‑3p expression promoted osteogenic differentiation. In addition, dual‑luciferase activity analysis and adenovirus infection experiments revealed that GATA binding protein 4 targeted miR‑144‑3p for regulation and that overexpression of GATA4 promoted the expression of miR‑144‑3p. These data indicated that miR‑144‑3p plays a role in inhibiting BMSC osteogenic differentiation and that GATA4 inhibits osteogenic differentiation by targeting miR‑144‑3p expression.
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Copy and paste a formatted citation
Spandidos Publications style
Qiu T, Li H, Lu T, Shu L, Chen C and Wang C: GATA4 regulates osteogenic differentiation by targeting miR‑144‑3p. Exp Ther Med 23: 83, 2022.
APA
Qiu, T., Li, H., Lu, T., Shu, L., Chen, C., & Wang, C. (2022). GATA4 regulates osteogenic differentiation by targeting miR‑144‑3p. Experimental and Therapeutic Medicine, 23, 83. https://doi.org/10.3892/etm.2021.11006
MLA
Qiu, T., Li, H., Lu, T., Shu, L., Chen, C., Wang, C."GATA4 regulates osteogenic differentiation by targeting miR‑144‑3p". Experimental and Therapeutic Medicine 23.1 (2022): 83.
Chicago
Qiu, T., Li, H., Lu, T., Shu, L., Chen, C., Wang, C."GATA4 regulates osteogenic differentiation by targeting miR‑144‑3p". Experimental and Therapeutic Medicine 23, no. 1 (2022): 83. https://doi.org/10.3892/etm.2021.11006
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu T, Li H, Lu T, Shu L, Chen C and Wang C: GATA4 regulates osteogenic differentiation by targeting miR‑144‑3p. Exp Ther Med 23: 83, 2022.
APA
Qiu, T., Li, H., Lu, T., Shu, L., Chen, C., & Wang, C. (2022). GATA4 regulates osteogenic differentiation by targeting miR‑144‑3p. Experimental and Therapeutic Medicine, 23, 83. https://doi.org/10.3892/etm.2021.11006
MLA
Qiu, T., Li, H., Lu, T., Shu, L., Chen, C., Wang, C."GATA4 regulates osteogenic differentiation by targeting miR‑144‑3p". Experimental and Therapeutic Medicine 23.1 (2022): 83.
Chicago
Qiu, T., Li, H., Lu, T., Shu, L., Chen, C., Wang, C."GATA4 regulates osteogenic differentiation by targeting miR‑144‑3p". Experimental and Therapeutic Medicine 23, no. 1 (2022): 83. https://doi.org/10.3892/etm.2021.11006
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