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Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia

  • Authors:
    • Jie Wang
    • Jun He
    • Yucheng Fan
    • Fangjing Xu
    • Qian Liu
    • Ruhua He
    • Ru Yan
  • View Affiliations / Copyright

    Affiliations: School of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R China, Department of Cardiovascular Internal Medicine, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R China, School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 85
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    Published online on: November 25, 2021
       https://doi.org/10.3892/etm.2021.11008
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Abstract

Mitochondrial malfunction leads to the remodeling of myocardial energy metabolism during myocardial ischemia (MI). However, the alterations to the mitochondrial proteome profile during this period has not yet been clarified. An acute MI model was established by high position ligation of the left anterior descending artery in 8‑week‑old C57BL/6N mice. After 15 min of ligation, the animals were euthanized, and their hearts were collected. The myocardial ultrastructure was observed using transmission electron microscopy (TEM). The cardiac mitochondrial proteome profile was analyzed by liquid chromatography‑tandem mass spectrometry (LC‑MS/MS) and bioinformatics analyses. TEM showed that the outer membrane of the mitochondria was dissolved, and the inner membrane (cristae) was corrupted and broken down extensively in the MI group. The mitochondrial membrane potential was decreased. More than 1,700 mitochondrial proteins were identified by LC‑MS/MS analysis, and 119 were differentially expressed. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes functional enrichment analysis showed that endopeptidase activity regulation, the mitochondrial inner membrane, oxidative phosphorylation, the hypoxia‑inducible factor‑1 signaling pathway, the pentose phosphate pathway and the peroxisome proliferator‑activated receptor signaling pathway were involved in the pathophysiological process in the early stage of acute MI. Extensive and substantial changes in the mitochondrial proteins as well as mitochondrial microstructural damage occur in the early stages of acute MI. In the present study, the series of proteins crucially involved in the pathways of mitochondrial dysfunction and metabolism were identified. Further studies are needed to clarify the roles of these proteins in myocardial metabolism remodeling during acute MI injury.
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Spandidos Publications style
Wang J, He J, Fan Y, Xu F, Liu Q, He R and Yan R: Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia. Exp Ther Med 23: 85, 2022.
APA
Wang, J., He, J., Fan, Y., Xu, F., Liu, Q., He, R., & Yan, R. (2022). Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia. Experimental and Therapeutic Medicine, 23, 85. https://doi.org/10.3892/etm.2021.11008
MLA
Wang, J., He, J., Fan, Y., Xu, F., Liu, Q., He, R., Yan, R."Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia". Experimental and Therapeutic Medicine 23.1 (2022): 85.
Chicago
Wang, J., He, J., Fan, Y., Xu, F., Liu, Q., He, R., Yan, R."Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia". Experimental and Therapeutic Medicine 23, no. 1 (2022): 85. https://doi.org/10.3892/etm.2021.11008
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Spandidos Publications style
Wang J, He J, Fan Y, Xu F, Liu Q, He R and Yan R: Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia. Exp Ther Med 23: 85, 2022.
APA
Wang, J., He, J., Fan, Y., Xu, F., Liu, Q., He, R., & Yan, R. (2022). Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia. Experimental and Therapeutic Medicine, 23, 85. https://doi.org/10.3892/etm.2021.11008
MLA
Wang, J., He, J., Fan, Y., Xu, F., Liu, Q., He, R., Yan, R."Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia". Experimental and Therapeutic Medicine 23.1 (2022): 85.
Chicago
Wang, J., He, J., Fan, Y., Xu, F., Liu, Q., He, R., Yan, R."Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia". Experimental and Therapeutic Medicine 23, no. 1 (2022): 85. https://doi.org/10.3892/etm.2021.11008
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