Long non‑coding RNA GAS6‑AS1 enhances breast cancer cell aggressiveness by functioning as a competing endogenous RNA of microRNA‑215‑5p to enhance SOX9 expression

Wu,Xiu-Ping; Xu,Zhi-Qiang; Xie,Wang-Mei; Lai,Yao-Long; He,Kai; Jiang,Yan; Xu,Zhen-Chao; Lin,Yi-Na; Xie,Yuan-Fu

doi:10.3892/etm.2021.11032

Long non‑coding RNA GAS6‑AS1 enhances breast cancer cell aggressiveness by functioning as a competing endogenous RNA of microRNA‑215‑5p to enhance SOX9 expression

Authors:
- Xiu-Ping Wu
- Zhi-Qiang Xu
- Wang-Mei Xie
- Yao-Long Lai
- Kai He
- Yan Jiang
- Zhen-Chao Xu
- Yi-Na Lin
- Yuan-Fu Xie
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Affiliations: Department of Breast Surgery, Zhengxing Hospital, Zhangzhou, Fujian 363000, P.R. China, Department of Radiation Oncology, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian 363000, P.R. China
Published online on: December 2, 2021 https://doi.org/10.3892/etm.2021.11032
Article Number: 109

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Abstract

Long non‑coding (lnc) RNAs play crucial functions in human cancer. However, until recently, the involvement of the lncRNA GAS6‑AS1 in breast cancer (BCa) malignancy has not been studied exhaustively. The roles and underlying mode of action of GAS6‑AS1 action in BCa progression were examined through functional experiments. A decline in GAS6‑AS1 level led to a significant decrease in BCa cell proliferation, and the ability for colony formation. Here, GAS6‑AS1 competed as endogenous RNA by sequestering microRNA‑215‑5p (miR‑215‑5p) causing an enhanced expression of SRY‑box transcription factor 9 (SOX9). The effects of silencing GAS6‑AS1 on BCa malignant phenotypes could be ameliorated by inhibiting miR‑215‑5p or restoring SOX9. Thus, GAS6‑AS1 acted as a lncRNA that drives tumor in BCa, and enabled progression of BCa through miR‑215‑5p /SOX9 axis regulation. These outcomes show that the GAS6‑AS1/miR‑215‑5p/SOX9 axis is a potentially effective target for cancer treatment and management.

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