Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
February-2022 Volume 23 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2022 Volume 23 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

miR‑27b attenuates dexamethasone‑inhibited proliferation and osteoblastic differentiation in MC3T3‑E1 cells by targeting PPARγ2

  • Authors:
    • Huicheng Lv
    • Tieyi Yang
    • Aimin He
    • Mingbo Wang
    • Haisheng Jia
    • Min Ma
    • Siqin Li
  • View Affiliations / Copyright

    Affiliations: Second Department of Trauma, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region 010000, P.R. China, Department of Ultrasound Medicine, People's Hospital of Inner Mongolia Autonomous Region, Hohhot, Inner Mongolia Autonomous Region 010020, P.R. China
    Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 127
    |
    Published online on: December 10, 2021
       https://doi.org/10.3892/etm.2021.11050
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Osteoporosis is a metabolic bone illness characterized by low bone density and a high risk of fracture. It is estimated that there are >60 million individuals in China suffering from this disease, which highlights an urgent requirement for the development of novel and safe drugs for the long‑term treatment of osteoporosis. MicroRNAs (miRNAs/miRs) have previously been identified as critical regulators in the progression of osteoporosis. As an intronic miRNA, miR‑27b enhances the osteoblastic differentiation of stem cells from the bone marrow and the maxillary sinus membrane. However, the mechanism underlying miR‑27b in osteoporosis remains to be elucidated. In the present study, MC3T3‑E1 pre‑osteoblasts were treated with dexamethasone (DEX) to establish an in vitro model of osteoporosis. The results of the present study demonstrated that DEX treatment markedly inhibited the viability of MC3T3‑E1 cells, and downregulated the expression level of miR‑27b. The results of reverse transcription‑quantitative PCR, western blotting and dual‑luciferase assays revealed that miR‑27b directly regulated and suppressed the expression of peroxisome proliferator‑activated receptor γ2 (PPARγ2) in MC3T3‑E1 cells. Furthermore, overexpression of miR‑27b by transfection of cells with miR‑27b mimic attenuated DEX‑mediated inhibition of cell viability, alkaline phosphatase (ALP) activity and the expression levels of bone morphogenetic protein‑2 (BMP2), runt‑related protein 2 (Runx2) and osteocalcin (OCN). The results of the present study indicated that miR‑27b alleviated DEX‑inhibited proliferation and osteoblastic differentiation. Moreover, miR‑27b knockdown repressed MC3T3‑E1 cell viability, ALP activity and protein levels of BMP2, Runx2 and OCN. However, these effects were abrogated by small interfering RNA‑mediated PPARγ2 silencing. In conclusion, the results of the present study demonstrated that miR‑27b attenuated DEX‑inhibited proliferation and osteoblastic differentiation in MC3T3‑E1 pre‑osteoblasts by targeting PPARγ2.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Gaffney-Stomberg E: The impact of trace minerals on bone metabolism. Biol Trace Elem Res. 188:26–34. 2019.PubMed/NCBI View Article : Google Scholar

2 

Kini U and Nandeesh B: Physiology of bone formation, remodeling, and metabolism. In: Radionuclide and Hybrid Bone Imaging. Fogelman I, Gnanasegaran G and van der Wall H (eds). Springer, Berlin, Heidelberg, pp29-57, 2012.

3 

Strålberg F, Kassem A, Kasprzykowski F, Abrahamson M, Grubb A, Lindholm C and Lerner UH: Inhibition of lipopolysaccharide-induced osteoclast formation and bone resorption in vitro and in vivo by cysteine proteinase inhibitors. J Leukoc Biol. 101:1233–1243. 2017.PubMed/NCBI View Article : Google Scholar

4 

Demontiero O, Vidal C and Duque G: Aging and bone loss: New insights for the clinician. Ther Adv Musculoskelet Dis. 4:61–76. 2012.PubMed/NCBI View Article : Google Scholar

5 

Jiang N and Xia W: Assessment of bone quality in patients with diabetes mellitus. Osteoporos Int. 29:1721–1736. 2018.PubMed/NCBI View Article : Google Scholar

6 

Lin X, Xiong D, Peng YQ, Sheng ZF, Wu XY, Wu XP, Wu F, Yuan LQ and Liao EY: Epidemiology and management of osteoporosis in the People's Republic of China: Current perspectives. Clin Interv Aging. 10:1017–1033. 2015.PubMed/NCBI View Article : Google Scholar

7 

Zeng Q, Li N, Wang Q, Feng J, Sun D, Zhang Q, Huang J, Wen Q, Hu R, Wang L, et al: The prevalence of osteoporosis in China, a nationwide, multicenter DXA survey. J Bone Miner Res. 34:1789–1797. 2019.PubMed/NCBI View Article : Google Scholar

8 

Cui Z, Meng X, Feng H, Zhuang S, Liu Z, Zhu T, Ye K, Xing Y, Sun C, Zhou F, et al: Estimation and projection about the standardized prevalence of osteoporosis in mainland China. Arch Osteoporos. 15(2)2019.PubMed/NCBI View Article : Google Scholar

9 

Skjødt MK, Frost M and Abrahamsen B: Side effects of drugs for osteoporosis and metastatic bone disease. Br J Clin Pharmacol. 85:1063–1071. 2019.PubMed/NCBI View Article : Google Scholar

10 

Li K, Chen S, Cai P, Chen K, Li L, Yang X, Yi J, Luo X, Du Y and Zheng H: miRNA-483-5p is involved in the pathogenesis of osteoporosis by promoting osteoclast differentiation. Mol Cell Probes. 49(101479)2020.PubMed/NCBI View Article : Google Scholar

11 

Li JY, Wei X, Sun Q, Zhao XQ, Zheng CY, Bai CX, Du J, Zhang Z, Zhu LG and Jia YS: MicroRNA-449b-5p promotes the progression of osteoporosis by inhibiting osteogenic differentiation of BMSCs via targeting Satb2. Eur Rev Med Pharmacol Sci. 23:6394–6403. 2019.PubMed/NCBI View Article : Google Scholar

12 

Kida K, Nakajima M, Mohri T, Oda Y, Takagi S, Fukami T and Yokoi T: PPARα is regulated by miR-21 and miR-27b in human liver. Pharm Res. 28:2467–2476. 2011.PubMed/NCBI View Article : Google Scholar

13 

Chen D, Si W, Shen J, Du C, Lou W, Bao C, Zheng H, Pan J, Zhong G, Xu L, et al: miR-27b-3p inhibits proliferation and potentially reverses multi-chemoresistance by targeting CBLB/GRB2 in breast cancer cells. Cell Death Dis. 9(188)2018.PubMed/NCBI View Article : Google Scholar

14 

Henriksen TI, Davidsen PK, Pedersen M, Schultz HS, Hansen NS, Larsen TJ, Vaag A, Pedersen BK, Nielsen S and Scheele C: Dysregulation of a novel miR-23b/27b-p53 axis impairs muscle stem cell differentiation of humans with type 2 diabetes. Mol Metab. 6:770–779. 2017.PubMed/NCBI View Article : Google Scholar

15 

Wu X, Yan T, Wang Z, Wu X, Cao G and Zhang C: lncRNA ZEB2-AS1 promotes bladder cancer cell proliferation and inhibits apoptosis by regulating miR-27b. Biomed Pharmacother. 96:299–304. 2017.PubMed/NCBI View Article : Google Scholar

16 

Han Z, Zhan R, Chen S, Deng J, Shi J and Wang W: miR-181b/Oncostatin m axis inhibits prostate cancer bone metastasis via modulating osteoclast differentiation. J Cell Biochem. 121:1664–1674. 2020.PubMed/NCBI View Article : Google Scholar

17 

Takigawa S, Chen A, Wan Q, Na S, Sudo A, Yokota H and Hamamura K: Role of miR-222-3p in c-Src-Mediated Regulation of Osteoclastogenesis. Int J Mol Sci. 17(240)2016.PubMed/NCBI View Article : Google Scholar

18 

Seenprachawong K, Tawornsawutruk T, Nantasenamat C, Nuchnoi P, Hongeng S and Supokawej A: miR-130a and miR-27b enhance osteogenesis in human bone marrow mesenchymal stem cells via specific down-regulation of peroxisome proliferator-activated receptor γ. Front Genet. 9(543)2018.PubMed/NCBI View Article : Google Scholar

19 

Peng W, Zhu S, Li X, Weng J and Chen S: miR-27b-3p suppressed osteogenic differentiation of maxillary sinus membrane stem cells by targeting Sp7. Implant Dent. 26:492–499. 2017.PubMed/NCBI View Article : Google Scholar

20 

Guo M, Li C, Lei Y, Xu S, Zhao D and Lu X-Y: Role of the adipose PPARγ-adiponectin axis in susceptibility to stress and depression/anxiety-related behaviors. Mol Psychiatry. 22:1056–1068. 2017.PubMed/NCBI View Article : Google Scholar

21 

Calvier L, Chouvarine P, Legchenko E, Hoffmann N, Geldner J, Borchert P, Jonigk D, Mozes MM and Hansmann G: PPARγ links BMP2 and TGFβ1 pathways in vascular smooth muscle cells, regulating cell proliferation and glucose metabolism. Cell Metab. 25:1118–1134.e1117. 2017.PubMed/NCBI View Article : Google Scholar

22 

Ye G, Gao H, Wang Z, Lin Y, Liao X, Zhang H, Chi Y, Zhu H and Dong S: PPARα and PPARγ activation attenuates total free fatty acid and triglyceride accumulation in macrophages via the inhibition of Fatp1 expression. Cell Death Dis. 10(39)2019.PubMed/NCBI View Article : Google Scholar

23 

Wan Y, Chong L-W and Evans RM: PPAR-γ regulates osteoclastogenesis in mice. Nat Med. 13:1496–1503. 2007.PubMed/NCBI View Article : Google Scholar

24 

Zhuang H, Zhang X, Zhu C, Tang X, Yu F, Shang GW and Cai X: Molecular mechanisms of PPAR-γ governing MSC osteogenic and adipogenic differentiation. Curr Stem Cell Res Ther. 11:255–264. 2016.PubMed/NCBI View Article : Google Scholar

25 

Xu J, Lv S, Hou Y, Xu K, Sun D, Zheng Y, Zhang Z, Li X, Li Y and Chi G: miR-27b promotes type II collagen expression by targetting peroxisome proliferator-activated receptor-γ2 during rat articular chondrocyte differentiation. Biosci Rep. 38(BSR20171109)2018.PubMed/NCBI View Article : Google Scholar

26 

Xu WN, Zheng HL, Yang RZ, Jiang LS and Jiang SD: HIF-1α Regulates Glucocorticoid-Induced Osteoporosis Through PDK1/AKT/mTOR Signaling Pathway. Front Endocrinol (Lausanne). 10(922)2020.PubMed/NCBI View Article : Google Scholar

27 

Li P, Mao WW, Zhang S, Zhang L, Chen ZR and Lu ZD: Sodium hydrosulfide alleviates dexamethasone-induced cell senescence and dysfunction through targeting the miR-22/sirt1 pathway in osteoblastic MC3T3-E1 cells. Exp Ther Med. 21(238)2021.PubMed/NCBI View Article : Google Scholar

28 

Yang L, Liu S, Mu S, Guo R, Zhou L and Fu Q: Paeoniflorin Attenuates Dexamethasone-Induced Apoptosis of Osteoblast Cells and Promotes Bone Formation via Regulating AKT/mTOR/Autophagy Signaling Pathway. Evid Based Complement Alternat Med. 2021(6623464)2021.PubMed/NCBI View Article : Google Scholar

29 

Zhu C, Zheng XF, Yang YH, Li B, Wang YR, Jiang SD and Jiang LS: LGR4 acts as a key receptor for R-spondin 2 to promote osteogenesis through Wnt signaling pathway. Cell Signal. 28:989–1000. 2016.PubMed/NCBI View Article : Google Scholar

30 

Gulyaeva LF and Kushlinskiy NE: Regulatory mechanisms of microRNA expression. J Transl Med. 14(143)2016.PubMed/NCBI View Article : Google Scholar

31 

Ge DW, Wang WW, Chen HT, Yang L and Cao XJ: Functions of microRNAs in osteoporosis. Eur Rev Med Pharmacol Sci. 21:4784–4789. 2017.PubMed/NCBI

32 

Chen R, Qiu H, Tong Y, Liao F, Hu X, Qiu Y and Liao Y: miRNA-19a-3p alleviates the progression of osteoporosis by targeting HDAC4 to promote the osteogenic differentiation of hMSCs. Biochem Biophys Res Commun. 516:666–672. 2019.PubMed/NCBI View Article : Google Scholar

33 

Li QQ, Wei Q, Zhai XC, Qin L, Li HB, Meng R and Chen SC: miRNA-7b-5p attenuates the progression of osteoporosis by inhibiting adipose differentiation of hMSCs via regulating IRS2. Eur Rev Med Pharmacol Sci. 23:9207–9214. 2019.PubMed/NCBI View Article : Google Scholar

34 

Lee JJ, Drakaki A, Iliopoulos D and Struhl K: miR-27b targets PPARγ to inhibit growth, tumor progression and the inflammatory response in neuroblastoma cells. Oncogene. 31:3818–3825. 2012.PubMed/NCBI View Article : Google Scholar

35 

Tao J, Zhi X, Zhang X, Fu M, Huang H, Fan Y, Guan W and Zou C: miR-27b-3p suppresses cell proliferation through targeting receptor tyrosine kinase like orphan receptor 1 in gastric cancer. J Exp Clin Cancer Res. 34(139)2015.PubMed/NCBI View Article : Google Scholar

36 

Wang J, Song Y, Zhang Y, Xiao H, Sun Q, Hou N, Guo S, Wang Y, Fan K, Zhan D, et al: Cardiomyocyte overexpression of miR-27b induces cardiac hypertrophy and dysfunction in mice. Cell Res. 22:516–527. 2012.PubMed/NCBI View Article : Google Scholar

37 

Hou N, Wang J, Li Z, Cao Y, Fan K and Yang X: Cardiomycyte overexpression of miR-27b resulted in cardiac fibrosis and mitochondria injury in mice. Yi Chuan. 34:326–334. 2012.PubMed/NCBI View Article : Google Scholar : (In Chinese).

38 

Li YF, Li SH, Liu Y and Luo YT: Long noncoding RNA CIR promotes chondrocyte extracellular matrix degradation in osteoarthritis by acting as a sponge for Mir-27b. Cell Physiol Biochem. 43:602–610. 2017.PubMed/NCBI View Article : Google Scholar

39 

Xu D, Gao Y, Hu N, Wu L and Chen Q: miR-365 Ameliorates Dexamethasone-Induced Suppression of Osteogenesis in MC3T3-E1 Cells by Targeting HDAC4. Int J Mol Sci. 18(977)2017.PubMed/NCBI View Article : Google Scholar

40 

Tang J, Yu H, Wang Y, Duan G, Wang B, Li W and Zhu Z: MicroRNA-199a counteracts glucocorticoid inhibition of bone marrow mesenchymal stem cell osteogenic differentiation through regulation of Klotho expression in vitro. Cell Biol Int. 44:2532–2540. 2020.PubMed/NCBI View Article : Google Scholar

41 

Shen GY, Ren H, Shang Q, Zhao WH, Zhang ZD, Yu X, Huang JJ, Tang JJ, Yang ZD, Liang D, et al: Let-7f-5p regulates TGFBR1 in glucocorticoid-inhibited osteoblast differentiation and ameliorates glucocorticoid-induced bone loss. Int J Biol Sci. 15:2182–2197. 2019.PubMed/NCBI View Article : Google Scholar

42 

Li H, Li T, Fan J, Li T, Fan L, Wang S, Weng X, Han Q and Zhao RC: miR-216a rescues dexamethasone suppression of osteogenesis, promotes osteoblast differentiation and enhances bone formation, by regulating c-Cbl-mediated PI3K/AKT pathway. Cell Death Differ. 22:1935–1945. 2015.PubMed/NCBI View Article : Google Scholar

43 

Corrales P, Izquierdo-Lahuerta A and Medina-Gómez G: Maintenance of kidney metabolic homeostasis by PPAR gamma. Int J Mol Sci. 19(2063)2018.PubMed/NCBI View Article : Google Scholar

44 

Karbiener M, Fischer C, Nowitsch S, Opriessnig P, Papak C, Ailhaud G, Dani C, Amri EZ and Scheideler M: MicroRNA miR-27b impairs human adipocyte differentiation and targets PPARgamma. Biochem Biophys Res Commun. 390:247–251. 2009.PubMed/NCBI View Article : Google Scholar

45 

Shockley KR, Lazarenko OP, Czernik PJ, Rosen CJ, Churchill GA and Lecka-Czernik B: PPARgamma2 nuclear receptor controls multiple regulatory pathways of osteoblast differentiation from marrow mesenchymal stem cells. J Cell Biochem. 106:232–246. 2009.PubMed/NCBI View Article : Google Scholar

46 

Guo L, Chen K, Yuan J, Huang P, Xu X, Li C, Qian N, Qi J, Shao Z, Deng L, et al: Estrogen inhibits osteoclasts formation and bone resorption via microRNA-27a targeting PPARγ and APC. J Cell Physiol. 234:581–594. 2018.PubMed/NCBI View Article : Google Scholar

47 

Patel JJ, Butters OR and Arnett TR: PPAR agonists stimulate adipogenesis at the expense of osteoblast differentiation while inhibiting osteoclast formation and activity. Cell Biochem Funct. 32:368–377. 2014.PubMed/NCBI View Article : Google Scholar

48 

Wang H and Shen Y: MicroRNA 20a negatively regulates the growth and osteoclastogenesis of THP 1 cells by downregulating PPARγ. Mol Med Rep. 20:4271–4276. 2019.PubMed/NCBI View Article : Google Scholar

49 

Wang Y, Pan Z and Chen F: Inhibition of PPARγ by bisphenol A diglycidyl ether ameliorates dexamethasone-induced osteoporosis in a mouse model. J Int Med Res. 47:6268–6277. 2019.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lv H, Yang T, He A, Wang M, Jia H, Ma M and Li S: miR‑27b attenuates dexamethasone‑inhibited proliferation and osteoblastic differentiation in MC3T3‑E1 cells by targeting PPARγ2. Exp Ther Med 23: 127, 2022.
APA
Lv, H., Yang, T., He, A., Wang, M., Jia, H., Ma, M., & Li, S. (2022). miR‑27b attenuates dexamethasone‑inhibited proliferation and osteoblastic differentiation in MC3T3‑E1 cells by targeting PPARγ2. Experimental and Therapeutic Medicine, 23, 127. https://doi.org/10.3892/etm.2021.11050
MLA
Lv, H., Yang, T., He, A., Wang, M., Jia, H., Ma, M., Li, S."miR‑27b attenuates dexamethasone‑inhibited proliferation and osteoblastic differentiation in MC3T3‑E1 cells by targeting PPARγ2". Experimental and Therapeutic Medicine 23.2 (2022): 127.
Chicago
Lv, H., Yang, T., He, A., Wang, M., Jia, H., Ma, M., Li, S."miR‑27b attenuates dexamethasone‑inhibited proliferation and osteoblastic differentiation in MC3T3‑E1 cells by targeting PPARγ2". Experimental and Therapeutic Medicine 23, no. 2 (2022): 127. https://doi.org/10.3892/etm.2021.11050
Copy and paste a formatted citation
x
Spandidos Publications style
Lv H, Yang T, He A, Wang M, Jia H, Ma M and Li S: miR‑27b attenuates dexamethasone‑inhibited proliferation and osteoblastic differentiation in MC3T3‑E1 cells by targeting PPARγ2. Exp Ther Med 23: 127, 2022.
APA
Lv, H., Yang, T., He, A., Wang, M., Jia, H., Ma, M., & Li, S. (2022). miR‑27b attenuates dexamethasone‑inhibited proliferation and osteoblastic differentiation in MC3T3‑E1 cells by targeting PPARγ2. Experimental and Therapeutic Medicine, 23, 127. https://doi.org/10.3892/etm.2021.11050
MLA
Lv, H., Yang, T., He, A., Wang, M., Jia, H., Ma, M., Li, S."miR‑27b attenuates dexamethasone‑inhibited proliferation and osteoblastic differentiation in MC3T3‑E1 cells by targeting PPARγ2". Experimental and Therapeutic Medicine 23.2 (2022): 127.
Chicago
Lv, H., Yang, T., He, A., Wang, M., Jia, H., Ma, M., Li, S."miR‑27b attenuates dexamethasone‑inhibited proliferation and osteoblastic differentiation in MC3T3‑E1 cells by targeting PPARγ2". Experimental and Therapeutic Medicine 23, no. 2 (2022): 127. https://doi.org/10.3892/etm.2021.11050
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team