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Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells

  • Authors:
    • Panpan Dong
    • Ganghui Ye
    • Xinzhuo Tu
    • Ying Luo
    • Weitong Cui
    • Yuxin Ma
    • Lei Wei
    • Xuewen Tian
    • Qinglu Wang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Biomedical Engineering and Technology of Shandong High School, Qilu Medical University, Zibo, Shandong 255213, P.R. China, Department of Clinical Laboratory, Zibo Central Hospital, Zibo, Shandong 255300, P.R. China, College of Sports and Health, Shandong Sport University, Jinan, Shandong 330013, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 164
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    Published online on: December 22, 2021
       https://doi.org/10.3892/etm.2021.11087
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Abstract

Bisphenol A (BPA) is a common industrial chemical widely used to produce various plastics and is known to impair neural stem cells (NSCs). However, the effects of low‑dose BPA exposure on the stemness maintenance and differentiation fate of NSCs remain unclear in the infant brain. The present study demonstrated that 1 µM BPA promoted human NSC proliferation and stemness, without significantly increasing apoptosis. The Chip‑seq experiments demonstrated that both the cell cycle and the TGF‑β signaling pathway were accelerated after treatment with 1 µM BPA. Subsequently, estrogen‑related receptor α (ERRα) gene knockout cell lines were constructed using CRISPR/Cas9. Further western blotting and chromatin immunoprecipitation‑PCR experiments demonstrated that BPA maintained cell stemness by binding to an EERα receptor and activating the TGF‑β1 signaling pathway, including the downstream factors Aurora kinases B and Id2. In conclusion, the stemness of NSCs could be maintained by BPA at 1 µM through the activation of the ERRα and TGF‑β1 signaling pathways and could restrain the differentiation of NSCs into neurons. The present research further clarified the mechanism of BPA toxicity on NSCs from the novel perspective of ERRα and TGF‑β1 signaling pathways regulated by BPA and provided insights into potential novel methods of prevention and therapy for neurogenic diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Dong P, Ye G, Tu X, Luo Y, Cui W, Ma Y, Wei L, Tian X and Wang Q: Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells. Exp Ther Med 23: 164, 2022.
APA
Dong, P., Ye, G., Tu, X., Luo, Y., Cui, W., Ma, Y. ... Wang, Q. (2022). Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells. Experimental and Therapeutic Medicine, 23, 164. https://doi.org/10.3892/etm.2021.11087
MLA
Dong, P., Ye, G., Tu, X., Luo, Y., Cui, W., Ma, Y., Wei, L., Tian, X., Wang, Q."Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells". Experimental and Therapeutic Medicine 23.2 (2022): 164.
Chicago
Dong, P., Ye, G., Tu, X., Luo, Y., Cui, W., Ma, Y., Wei, L., Tian, X., Wang, Q."Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells". Experimental and Therapeutic Medicine 23, no. 2 (2022): 164. https://doi.org/10.3892/etm.2021.11087
Copy and paste a formatted citation
x
Spandidos Publications style
Dong P, Ye G, Tu X, Luo Y, Cui W, Ma Y, Wei L, Tian X and Wang Q: Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells. Exp Ther Med 23: 164, 2022.
APA
Dong, P., Ye, G., Tu, X., Luo, Y., Cui, W., Ma, Y. ... Wang, Q. (2022). Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells. Experimental and Therapeutic Medicine, 23, 164. https://doi.org/10.3892/etm.2021.11087
MLA
Dong, P., Ye, G., Tu, X., Luo, Y., Cui, W., Ma, Y., Wei, L., Tian, X., Wang, Q."Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells". Experimental and Therapeutic Medicine 23.2 (2022): 164.
Chicago
Dong, P., Ye, G., Tu, X., Luo, Y., Cui, W., Ma, Y., Wei, L., Tian, X., Wang, Q."Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells". Experimental and Therapeutic Medicine 23, no. 2 (2022): 164. https://doi.org/10.3892/etm.2021.11087
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