search button
Open Access

Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells

  • Authors:
    • Panpan Dong
    • Ganghui Ye
    • Xinzhuo Tu
    • Ying Luo
    • Weitong Cui
    • Yuxin Ma
    • Lei Wei
    • Xuewen Tian
    • Qinglu Wang

  • View Affiliations

  • Published online on: December 22, 2021     https://doi.org/10.3892/etm.2021.11087
  • Article Number: 164

  • Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Bisphenol A (BPA) is a common industrial chemical widely used to produce various plastics and is known to impair neural stem cells (NSCs). However, the effects of low‑dose BPA exposure on the stemness maintenance and differentiation fate of NSCs remain unclear in the infant brain. The present study demonstrated that 1 µM BPA promoted human NSC proliferation and stemness, without significantly increasing apoptosis. The Chip‑seq experiments demonstrated that both the cell cycle and the TGF‑β signaling pathway were accelerated after treatment with 1 µM BPA. Subsequently, estrogen‑related receptor α (ERRα) gene knockout cell lines were constructed using CRISPR/Cas9. Further western blotting and chromatin immunoprecipitation‑PCR experiments demonstrated that BPA maintained cell stemness by binding to an EERα receptor and activating the TGF‑β1 signaling pathway, including the downstream factors Aurora kinases B and Id2. In conclusion, the stemness of NSCs could be maintained by BPA at 1 µM through the activation of the ERRα and TGF‑β1 signaling pathways and could restrain the differentiation of NSCs into neurons. The present research further clarified the mechanism of BPA toxicity on NSCs from the novel perspective of ERRα and TGF‑β1 signaling pathways regulated by BPA and provided insights into potential novel methods of prevention and therapy for neurogenic diseases.
View Figures
View References

Related Articles

Journal Cover

February-2022
Volume 23 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 2.7
  • Ranked Medicine Research and Experimental
    (total number of cites)
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Dong P, Ye G, Tu X, Luo Y, Cui W, Ma Y, Wei L, Tian X and Wang Q: Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells. Exp Ther Med 23: 164, 2022
APA
Dong, P., Ye, G., Tu, X., Luo, Y., Cui, W., Ma, Y. ... Wang, Q. (2022). Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells. Experimental and Therapeutic Medicine, 23, 164. https://doi.org/10.3892/etm.2021.11087
MLA
Dong, P., Ye, G., Tu, X., Luo, Y., Cui, W., Ma, Y., Wei, L., Tian, X., Wang, Q."Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells". Experimental and Therapeutic Medicine 23.2 (2022): 164.
Chicago
Dong, P., Ye, G., Tu, X., Luo, Y., Cui, W., Ma, Y., Wei, L., Tian, X., Wang, Q."Roles of ERRα and TGF‑β signaling in stemness enhancement induced by 1 <em>µ</em>M bisphenol A exposure via human neural stem cells". Experimental and Therapeutic Medicine 23, no. 2 (2022): 164. https://doi.org/10.3892/etm.2021.11087