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miR‑206 serves an important role in polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis

  • Authors:
    • Jie Zhou
    • Xuejing Jin
    • Zhumei Sheng
    • Zhifen Zhang
  • View Affiliations / Copyright

    Affiliations: Reproductive Endocrine Center, Hangzhou Women's Hospital, Hangzhou, Zhejiang 310008, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 179
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    Published online on: January 5, 2021
       https://doi.org/10.3892/etm.2021.9610
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Abstract

An increasing number of studies have reported that microRNAs (miRNAs) have an important role in polycystic ovary syndrome (PCOS). Downregulation of miR‑206 in patients with PCOS has been found, however, its specific role remains unclear. The present study aimed to investigate the roles of miR‑206 in (PCOS) and to determine the underlying molecular mechanisms. Reverse transcription‑quantitative PCR (RT‑qPCR) was performed to analyze the expression levels of miR‑206 in normal ovarian surface epithelial IOSE80 cells and human ovarian granulosa cell‑like KGN cells. TargetScan was used to predict the target gene of miR‑206, which was subsequently verified using a dual‑luciferase reporter gene assay. The mRNA expression levels of cyclin D2 (CCND2) and the transfection efficiencies of the miR‑206 mimic and CCDN2 overexpression plasmid were determined using RT‑qPCR analysis. The protein expression levels of CCND2, cleaved‑caspase‑3 and pro‑caspase‑3 were analyzed using western blotting, and an MTT assay and flow cytometric analysis were used to evaluate the cell viability and levels of apoptosis, respectively, in the cells following transfection. Finally, the activity of caspase‑3 was analyzed using a caspase‑3 activity assay kit. The results of the present study revealed that the expression levels of miR‑206 were downregulated in KGN cells compared with IOSE80 cells. CCND2 was predicted and verified to be a direct target gene of miR‑206, and the mRNA and protein expression levels of CCND2 were discovered to be upregulated in KGN cells compared with IOSE80 cells. The miR‑206 mimic and CCND2 overexpression plasmid significantly upregulated the expression levels of miR‑206 and CCND2, respectively, in KGN cells. The miR‑206 mimic also downregulated the expression levels of CCND2 in KGN cells, while this effect was reversed following the transfection with the CCND2 overexpression plasmid. Compared with the mimic control group, the miR‑206 mimic significantly decreased the cell viability, induced the levels of apoptosis, increased the activity of caspase‑3, upregulated cleaved‑caspase‑3 protein expression levels and downregulated pro‑caspase‑3 protein expression levels in KGN cells following transfection; these effects were reversed following the overexpression of CCND2. In conclusion, the findings of the present study suggested that miR‑206 may serve an important role in PCOS through modulating ovarian granulosa cell viability and apoptosis.
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1 

Macut D, Bjekić-Macut J, Rahelić D and Doknić M: Insulin and the polycystic ovary syndrome. Diabetes Res Clin Pract. 130:163–170. 2017.PubMed/NCBI View Article : Google Scholar

2 

Franik S, Eltrop SM, Kremer JA, Kiesel L and Farquhar C: Aromatase inhibitors (letrozole) for subfertile women with polycystic ovary syndrome. Cochrane Database Syst Rev. 5(CD010287)2018.PubMed/NCBI View Article : Google Scholar

3 

Öztürk A, Kucur SK, Seven A, Deveci E, Şencan H, Yilmaz O and Kiliç A: Temperament and character differences of patients with polycystic ovary syndrome. J Gynecol Obstet Hum Reprod. 48:255–259. 2019.PubMed/NCBI View Article : Google Scholar

4 

Cooney LG and Dokras A: Beyond fertility: Polycystic ovary syndrome and long-term health. Fertil Steril. 110:794–809. 2018.PubMed/NCBI View Article : Google Scholar

5 

Kshetrimayum C, Sharma A, Mishra VV and Kumar S: Polycystic ovarian syndrome: Environmental/occupational, lifestyle factors; an overview. J Turk Ger Gynecol Assoc. 20:255–263. 2019.PubMed/NCBI View Article : Google Scholar

6 

Sathyapalan T, Shepherd J, Arnett C, Coady AM, Kilpatrick ES and Atkin SL: Atorvastatin increases 25-hydroxy vitamin D concentrations in patients with polycystic ovary syndrome. Clin Chem. 56:1696–1700. 2020.PubMed/NCBI View Article : Google Scholar

7 

Li L, Zhang R, Zeng J, Ke H, Peng X, Huang L, Zhang H, Chen Z, Li TT, Tan Q, et al: Effectiveness and safety assessment of drospirenone/ethinyl estradiol tablet in treatment of PCOS patients: A single center, prospective, observational study. BMC Womens Health. 20(39)2020.PubMed/NCBI View Article : Google Scholar

8 

Xu J, Bao X, Peng Z, Wang L, Du L, Niu W and Sun Y: Comprehensive analysis of genome-wide DNA methylation across human polycystic ovary syndrome ovary granulosa cell. Oncotarget. 7:27899–27909. 2016.PubMed/NCBI View Article : Google Scholar

9 

Lai Q, Xiang W, Li Q, Zhang H, Li Y, Zhu G, Xiong C and Jin L: Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with polycystic ovary syndrome. Front Med. 12:518–524. 2018.PubMed/NCBI View Article : Google Scholar

10 

Lv X, He C, Huang C, Wang H, Hua G, Wang Z, Zhou J, Chen X, Ma B, Timm BK, et al: Timely expression and activation of YAP1 in granulosa cells is essential for ovarian follicle development. FASEB J. 33:10049–10064. 2019.PubMed/NCBI View Article : Google Scholar

11 

Fan Y, Chang Y, Wei L, Chen J, Li J, Goldsmith S, Silber S and Liang X: Apoptosis of mural granulosa cells is increased in women with diminished ovarian reserve. Version 2. J Assist Reprod Genet. 36:1225–1235. 2019.PubMed/NCBI View Article : Google Scholar

12 

Fu X, He Y, Wang X, Peng D, Chen X, Li X and Wan Q: MicroRNA-16 promotes ovarian granulosa cell proliferation and suppresses apoptosis through targeting PDCD4 in polycystic ovarian syndrome. Cell Physiol Biochem. 48:670–682. 2018.PubMed/NCBI View Article : Google Scholar

13 

Zheng Q, Li Y, Zhang D, Cui X, Dai K, Yang Y, Liu S, Tan J and Yan Q: ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats. Cell Death Dis. 8(e3145)2017.PubMed/NCBI View Article : Google Scholar

14 

Li M, Zhao H, Zhao SG, Wei DM, Zhao YR, Huang T, Muhammad T, Yan L, Gao F, Li L, et al: The HMGA2-IMP2 pathway promotes granulosa cell proliferation in polycystic ovary syndrome. J Clin Endocrinol Metab. 104:1049–1059. 2019.PubMed/NCBI View Article : Google Scholar

15 

Wang J, Chen J and Sen S: MicroRNA as biomarkers and diagnostics. J Cell Physiol. 231:25–30. 2016.PubMed/NCBI View Article : Google Scholar

16 

Yanaihara N and Harris CC: MicroRNA involvement in human cancers. Clin Chem. 59:1811–1812. 2013.PubMed/NCBI View Article : Google Scholar

17 

Liao XB, Perez VA, Król M, Yeh CH and Yuan LQ: MicroRNA and cardiovascular disease. Biomed Res Int. 2015(734380)2015.PubMed/NCBI View Article : Google Scholar

18 

Gong CL, Wang Y, Lyu Y, Sun JJ and Li GP: The effect and mechanism of microRNA199 in gynecological diseases. J Fam Plam Reprod H. 33:60–63. 2014.

19 

Guo J, Sun M, Teng X and Xu L: MicroRNA-7-5p regulates the expression of TFF3 in inflammatory bowel disease. Mol Med Rep. 16:1200–1206. 2017.PubMed/NCBI View Article : Google Scholar

20 

Wang M, Sun J, Xu B, Chrusciel M, Gao J, Bazert M, Stelmaszewska J, Xu Y, Zhang H, Pawelczyk L, et al: Functional characterization of microRNA-27a-3p expression in human polycystic ovary syndrome. Endocrinology. 159:297–309. 2018.PubMed/NCBI View Article : Google Scholar

21 

Sørensen AE, Wissing ML, Englund AL and Dalgaard LT: MicroRNA species in follicular fluid associating with polycystic ovary syndrome and related intermediary phenotypes. J Clin Endocrinol Metab. 101:1579–1589. 2016.PubMed/NCBI View Article : Google Scholar

22 

Roth LW, McCallie B, Alvero R, Schoolcraft WB, Minjarez D and Katz-Jaffe MG: Altered microRNA and gene expression in the follicular fluid of women with polycystic ovary syndrome. J Assist Reprod Genet. 31:355–362. 2014.PubMed/NCBI View Article : Google Scholar

23 

Ilie IR and Georgescu CE: Polycystic ovary syndrome-epigenetic mechanisms and aberrant microRNA. Adv Clin Chem. 71:25–45. 2015.PubMed/NCBI View Article : Google Scholar

24 

Díaz M, Bassols J, López-Bermejo A, de Zegher F and Ibáñez L: Low circulating levels of miR-451a in girls with polycystic ovary syndrome: Different effects of randomized treatments. J Clin Endocrinol Metab. 105:1–9. 2020.PubMed/NCBI View Article : Google Scholar

25 

Sun X, Su S, Zhang G, Zhang H and Yu X: MiR-204 suppresses cell proliferation and promotes apoptosis in ovarian granulosa cells via targeting TPT1 in polycystic ovary syndrome. Biochem Cell Biol. 97:554–562. 2019.PubMed/NCBI View Article : Google Scholar

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

27 

Bahri Khomami M, Boyle JA, Tay CT, Vanky E, Teede HJ, Joham AE and Moran LJ: Polycystic ovary syndrome and adverse pregnancy outcomes: Current state of knowledge, challenges and potential implications for practice. Clin Endocrinol (Oxf). 88:761–769. 2018.PubMed/NCBI View Article : Google Scholar

28 

Cui Y, Shi Y, Cui L, Han T, Gao X and Chen ZJ: Age-specific serum antimullerian hormone levels in women with and without polycystic ovary syndrome. Fertil Steril. 102:230–236. 2014.PubMed/NCBI View Article : Google Scholar

29 

Wei D, Xie J, Yin B, Hao H, Song X, Liu Q, Zhang C and Sun Y: Significantly lengthened telomere in granulosa cells from women with polycystic ovarian syndrome (PCOS). J Assist Reprod Genet. 34:861–866. 2017.PubMed/NCBI View Article : Google Scholar

30 

Chang L, Guo R, Yuan Z, Shi H and Zhang D: LncRNA HOTAIR regulates CCND1 and CCND2 expression by sponging miR-206 in ovarian cancer. Cell Physiol Biochem. 49:1289–1303. 2018.PubMed/NCBI View Article : Google Scholar

31 

Van Opdenbosch N and Lamkanfi M: Caspases in cell death, inflammation, and disease. Immunity. 50:1352–1364. 2019.PubMed/NCBI View Article : Google Scholar

32 

Pang X, Li K, Wei L, Huang Y, Su M, Wang L, Cao H and Chen T: IL-8 inhibits the apoptosis of MCF-7 human breast cancer cells by up-regulating Bcl-2 and down-regulating caspase-3. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 31:307–311. 2015.PubMed/NCBI(In Chinese).

33 

Zhang Y, Jiang Q, Wang N, Dai B, Chen Y and He L: Effects of taspine on proliferation and apoptosis by regulating caspase-3 expression and the ratio of Bax/Bcl-2 in A431 cells. Phytother Res. 25:357–364. 2011.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhou J, Jin X, Sheng Z and Zhang Z: miR‑206 serves an important role in polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis. Exp Ther Med 21: 179, 2021.
APA
Zhou, J., Jin, X., Sheng, Z., & Zhang, Z. (2021). miR‑206 serves an important role in polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis. Experimental and Therapeutic Medicine, 21, 179. https://doi.org/10.3892/etm.2021.9610
MLA
Zhou, J., Jin, X., Sheng, Z., Zhang, Z."miR‑206 serves an important role in polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis". Experimental and Therapeutic Medicine 21.3 (2021): 179.
Chicago
Zhou, J., Jin, X., Sheng, Z., Zhang, Z."miR‑206 serves an important role in polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis". Experimental and Therapeutic Medicine 21, no. 3 (2021): 179. https://doi.org/10.3892/etm.2021.9610
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Jin X, Sheng Z and Zhang Z: miR‑206 serves an important role in polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis. Exp Ther Med 21: 179, 2021.
APA
Zhou, J., Jin, X., Sheng, Z., & Zhang, Z. (2021). miR‑206 serves an important role in polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis. Experimental and Therapeutic Medicine, 21, 179. https://doi.org/10.3892/etm.2021.9610
MLA
Zhou, J., Jin, X., Sheng, Z., Zhang, Z."miR‑206 serves an important role in polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis". Experimental and Therapeutic Medicine 21.3 (2021): 179.
Chicago
Zhou, J., Jin, X., Sheng, Z., Zhang, Z."miR‑206 serves an important role in polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis". Experimental and Therapeutic Medicine 21, no. 3 (2021): 179. https://doi.org/10.3892/etm.2021.9610
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