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FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ

  • Authors:
    • Xiao-Lan Yang
    • Jian-Hua Mi
    • Qing Dong
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201100, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 181
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    Published online on: January 5, 2021
       https://doi.org/10.3892/etm.2021.9612
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Abstract

Ischemic stroke is a life‑threatening complication with a high rate of morbidity. Circulating fatty acid binding protein 4 (FABP4) has been reported to be associated with the outcome of acute ischemic stroke. The present study aimed to illustrate the function of FABP4 in ischemic stroke. PC12 cells exposed to oxygen glucose deprivation/reoxygenation (OGD/R) were used to mimic ischemia‑reperfusion (I/R) injury in ischemic stroke. Cell viability was estimated using a Cell Counting kit‑8 assay. The expression of FABP4 in PC12 cells under OGD/R was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). PC12 cells were transfected with FABP4 small interfering RNA (siRNA), inflammatory cytokines and reactive oxygen species (ROS) were determined via RT‑qPCR and ROS assay kit. Western blotting was performed to detect endoplasmic reticulum stress (ERS)‑related proteins and peroxisome proliferator‑activated receptor γ (PPARγ). Flow cytometry was used to evaluate the cell apoptotic rate. The expression of FABP4 increased gradually with the prolongation of reoxygenation within 8 h. FABP4‑knockdown inhibited the transcription of inflammatory cytokines, the production of ROS and decreased cell apoptosis. Furthermore, decreased ERS‑related proteins and increased PPARγ were estimated in PC12 cells transfected with FABP4 siRNA. PPARγ inhibitor GW9662 weakened the anti‑apoptotic effect of FABP4‑knockdown. Taken together, these results indicated that FABP4‑knockdown suppressed cell apoptosis via relieving ERS; this effect was reversed by treatment of GW9662.
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1 

Khoshnam SE, Winlow W, Farzaneh M, Farbood Y and Moghaddam HF: Pathogenic mechanisms following ischemic stroke. Neurol Sci. 38:1167–1186. 2017.PubMed/NCBI View Article : Google Scholar

2 

Siniscalchi A, Gallelli L, Malferrari G, Pirritano D, Serra R, Santangelo E and De Sarro G: Cerebral stroke injury: The role of cytokines and brain inflammation. J Basic Clin Physiol Pharmacol. 25:131–137. 2014.PubMed/NCBI View Article : Google Scholar

3 

Tobin MK, Bonds JA, Minshall RD, Pelligrino DA, Testai FD and Lazarov O: Neurogenesis and inflammation after ischemic stroke: What is known and where we go from here. J Cereb Blood Flow Metab. 34:1573–1584. 2014.PubMed/NCBI View Article : Google Scholar

4 

Kim JY, Kawabori M and Yenari MA: Innate inflammatory responses in stroke: Mechanisms and potential therapeutic targets. Curr Medb Chem. 21:2076–2097. 2014.PubMed/NCBI View Article : Google Scholar

5 

Blakeley JO and Llinas RH: Thrombolytic therapy for acute ischemic stroke. J Neurol Sci. 261:55–62. 2007.PubMed/NCBI View Article : Google Scholar

6 

Fu Y, Liu Q, Anrather J and Shi FD: Immune interventions in stroke. Nat Rev Neurol. 11:524–535. 2015.PubMed/NCBI View Article : Google Scholar

7 

Danton GH and Dietrich WD: Inflammatory mechanisms after ischemia and stroke. J Neuropathol Exp Neurol. 62:127–136. 2003.PubMed/NCBI View Article : Google Scholar

8 

Bogdan D, Falcone J, Kanjiya MP, Park SH, Carbonetti G, Studholme K, Gomez M, Lu Y, Elmes MW, Smietalo N, et al: Fatty acid-binding protein 5 controls microsomal prostaglandin E synthase 1 (mPGES-1) induction during inflammation. J Biol Chem. 293:5295–5306. 2018.PubMed/NCBI View Article : Google Scholar

9 

Yu CW, Liang X, Lipsky S, Karaaslan C, Kozakewich H, Hotamisligil GS, Bischoff J and Cataltepe S: Dual role of fatty acid-binding protein 5 on endothelial cell fate: A potential link between lipid metabolism and angiogenic responses. Angiogenesis. 19:95–106. 2016.PubMed/NCBI View Article : Google Scholar

10 

Rodriguez Sawicki L, Bottasso Arias NM, Scaglia N, Falomir Lockhart LJ, Franchini GR, Storch J and Córsico B: FABP1 knockdown in human enterocytes impairs proliferation and alters lipid metabolism. Biochim Biophys Acta Mol Cell Biol Lipids. 1862:1587–1594. 2017.PubMed/NCBI View Article : Google Scholar

11 

Hotamisligil GS and Bernlohr DA: Metabolic functions of FABPs-mechanisms and therapeutic implications. Nat Rev Endocrinol. 11:592–605. 2015.PubMed/NCBI View Article : Google Scholar

12 

Chen Y, Gao H, Yin Q, Chen L, Dong P, Zhang X and Kang J: ER stress activating ATF4/CHOP-TNF-α signaling pathway contributes to alcohol-induced disruption of osteogenic lineage of multipotential mesenchymal stem cell. Cell Physiol Biochem. 32:743–754. 2013.PubMed/NCBI View Article : Google Scholar

13 

Shi M, Huang R, Guo F, Li L, Feng Y, Wei Z, Zhou L, Ma L and Fu P: Pharmacological inhibition of fatty acid-binding protein 4 (FABP4) protects against renal ischemia-reperfusion injury. Rsc Advances. 8:15207–15214. 2018.PubMed/NCBI View Article : Google Scholar

14 

Hu B, Guo Y, Garbacz WG, Jiang M, Xu M, Huang H, Tsung A, Billiar TR, Ramakrishnan SK, Shah YM, et al: Fatty acid binding protein-4 (FABP4) is a hypoxia inducible gene that sensitizes mice to liver ischemia/reperfusion injury. J Hepatol. 63:855–862. 2015.PubMed/NCBI View Article : Google Scholar

15 

Holm S, Ueland T, Dahl TB, Michelsen AE, Skjelland M, Russell D, Nymo SH, Krohg-Sørensen K, Clausen OP, Atar D, et al: Fatty acid binding protein 4 is associated with carotid atherosclerosis and outcome in patients with acute ischemic stroke. PLoS One. 6(e28785)2011.PubMed/NCBI View Article : Google Scholar

16 

Li TF, Ma J, Han XW, Jia YX, Yuan HF, Shui SF, Guo D and Yan L: Chrysin ameliorates cerebral ischemia/reperfusion (I/R) injury in rats by regulating the PI3K/Akt/mTOR pathway. Neurochem Int. 129(104496)2019.PubMed/NCBI View Article : Google Scholar

17 

Jiang D, Sun X, Wang S and Man H: Upregulation of miR-874-3p decreases cerebral ischemia/reperfusion injury by directly targeting BMF and BCL2L13. Biomed Pharmacother. 117(108941)2019.PubMed/NCBI View Article : Google Scholar

18 

Whiley H and Taylor M: Legionella detection by culture and qPCR: Comparing apples and oranges. Crit Rev Microbiol. 42:65–74. 2016.PubMed/NCBI View Article : Google Scholar

19 

Zhang HF, Li TB, Liu B, Lou Z, Zhang JJ, Peng JJ, Zhang XJ, Ma QL, Peng J and Luo XJ: Inhibition of myosin light chain kinase reduces NADPH oxidase-mediated oxidative injury in rat brain following cerebral ischemia/reperfusion. Naunyn Schmiedebergs Arch Pharmacol. 388:953–963. 2015.PubMed/NCBI View Article : Google Scholar

20 

Tan Z, Guo F, Huang Z, Xia Z, Liu J, Tao S, Li L, Feng Y, Du X, Ma L and Fu P: Pharmacological and genetic inhibition of fatty acid-binding protein 4 alleviated cisplatin-induced acute kidney injury. J Cell Mol Med. 23:6260–6270. 2019.PubMed/NCBI View Article : Google Scholar

21 

Yang W and Paschen W: Unfolded protein response in brain ischemia: A timely update. J Cereb Blood Flow Metab. 36:2044–2050. 2016.PubMed/NCBI View Article : Google Scholar

22 

Chen Y, Liu S and Chen G: Aggravation of cerebral ischemia/reperfusion injury by peroxisome proliferator-activated receptor-gamma deficiency via endoplasmic reticulum stress. Med Sci Monit. 25:7518–7526. 2019.PubMed/NCBI View Article : Google Scholar

23 

Deng T, Wang Y, Wang C and Yan H: FABP4 silencing ameliorates hypoxia reoxygenation injury through the attenuation of endoplasmic reticulum stress-mediated apoptosis by activating PI3K/Akt pathway. Life Sci. 224:149–156. 2019.PubMed/NCBI View Article : Google Scholar

24 

Wu L, Xiong X, Wu X, Ye Y, Jian Z, Zhi Z and Gu L: Targeting oxidative stress and inflammation to prevent ischemia-reperfusion injury. Front Mol Neurosci. 13(28)2020.PubMed/NCBI View Article : Google Scholar

25 

Bosquet A, Guaita-Esteruelas S, Saavedra P, et al: Exogenous FABP4 induces endoplasmic reticulum stress in HepG2 liver cells. Atherosclerosis. 249:191–199. 2016.PubMed/NCBI View Article : Google Scholar

26 

Bosquet A, Girona J, Guaita-Esteruelas S, et al: FABP4 inhibitor BMS309403 decreases saturated-fatty-acid-induced endoplasmic reticulum stress-associated inflammation in skeletal muscle by reducing p38 MAPK activation. Biochimica et biophysica acta. Molecular and cell biology of lipids. 1863:604–613. 2018.PubMed/NCBI View Article : Google Scholar

27 

Qiao Y, Liu L, Yin L, Xu L, Tang Z, Qi Y, Mao Z, Zhao Y, Ma X and Peng J: FABP4 contributes to renal interstitial fibrosis via mediating inflammation and lipid metabolism. Cell Death Dis. 10(382)2019.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Mi J and Dong Q: FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ. Exp Ther Med 21: 181, 2021.
APA
Yang, X., Mi, J., & Dong, Q. (2021). FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ. Experimental and Therapeutic Medicine, 21, 181. https://doi.org/10.3892/etm.2021.9612
MLA
Yang, X., Mi, J., Dong, Q."FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ". Experimental and Therapeutic Medicine 21.3 (2021): 181.
Chicago
Yang, X., Mi, J., Dong, Q."FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ". Experimental and Therapeutic Medicine 21, no. 3 (2021): 181. https://doi.org/10.3892/etm.2021.9612
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Mi J and Dong Q: FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ. Exp Ther Med 21: 181, 2021.
APA
Yang, X., Mi, J., & Dong, Q. (2021). FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ. Experimental and Therapeutic Medicine, 21, 181. https://doi.org/10.3892/etm.2021.9612
MLA
Yang, X., Mi, J., Dong, Q."FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ". Experimental and Therapeutic Medicine 21.3 (2021): 181.
Chicago
Yang, X., Mi, J., Dong, Q."FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ". Experimental and Therapeutic Medicine 21, no. 3 (2021): 181. https://doi.org/10.3892/etm.2021.9612
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