Open Access

FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ

  • Authors:
    • Xiao-Lan Yang
    • Jian-Hua Mi
    • Qing Dong
  • View Affiliations

  • Published online on: January 5, 2021     https://doi.org/10.3892/etm.2021.9612
  • Article Number: 181
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ischemic stroke is a life‑threatening complication with a high rate of morbidity. Circulating fatty acid binding protein 4 (FABP4) has been reported to be associated with the outcome of acute ischemic stroke. The present study aimed to illustrate the function of FABP4 in ischemic stroke. PC12 cells exposed to oxygen glucose deprivation/reoxygenation (OGD/R) were used to mimic ischemia‑reperfusion (I/R) injury in ischemic stroke. Cell viability was estimated using a Cell Counting kit‑8 assay. The expression of FABP4 in PC12 cells under OGD/R was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). PC12 cells were transfected with FABP4 small interfering RNA (siRNA), inflammatory cytokines and reactive oxygen species (ROS) were determined via RT‑qPCR and ROS assay kit. Western blotting was performed to detect endoplasmic reticulum stress (ERS)‑related proteins and peroxisome proliferator‑activated receptor γ (PPARγ). Flow cytometry was used to evaluate the cell apoptotic rate. The expression of FABP4 increased gradually with the prolongation of reoxygenation within 8 h. FABP4‑knockdown inhibited the transcription of inflammatory cytokines, the production of ROS and decreased cell apoptosis. Furthermore, decreased ERS‑related proteins and increased PPARγ were estimated in PC12 cells transfected with FABP4 siRNA. PPARγ inhibitor GW9662 weakened the anti‑apoptotic effect of FABP4‑knockdown. Taken together, these results indicated that FABP4‑knockdown suppressed cell apoptosis via relieving ERS; this effect was reversed by treatment of GW9662.
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March-2021
Volume 21 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Yang X, Mi J and Dong Q: FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ. Exp Ther Med 21: 181, 2021
APA
Yang, X., Mi, J., & Dong, Q. (2021). FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ. Experimental and Therapeutic Medicine, 21, 181. https://doi.org/10.3892/etm.2021.9612
MLA
Yang, X., Mi, J., Dong, Q."FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ". Experimental and Therapeutic Medicine 21.3 (2021): 181.
Chicago
Yang, X., Mi, J., Dong, Q."FABP4 alleviates endoplasmic reticulum stress‑mediated ischemia‑reperfusion injury in PC12 cells via regulation of PPARγ". Experimental and Therapeutic Medicine 21, no. 3 (2021): 181. https://doi.org/10.3892/etm.2021.9612