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Experimental pretreatment with YES‑10®, a plant extract rich in scutellarin and chlorogenic acid, protects hippocampal neurons from ischemia/reperfusion injury via antioxidant role

  • Authors:
    • Young Eun Park
    • Yoohun Noh
    • Dae Won Kim
    • Tae-Kyeong Lee
    • Ji Hyeon Ahn
    • Bora Kim
    • Jae-Chul Lee
    • Cheol Woo Park
    • Joon Ha Park
    • Jong Dai Kim
    • Young-Myeong Kim
    • Il Jun Kang
    • Ji-Won Lee
    • Sung-Su Kim
    • Moo-Ho Won
  • View Affiliations / Copyright

    Affiliations: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Anatomy and Cell Biology and Neurology, College of Medicine, Chung‑Ang University, Seoul 06974, Republic of Korea, Department of Biochemistry and Molecular Biology and Research Institute of Oral Sciences, College of Dentistry, Gangnung‑Wonju National University, Gangneung, Gangwon 25457, Republic of Korea, Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Anatomy, College of Korean Medicine, Dongguk University, Gyeongju, Gyeongbuk 38066, Republic of Korea, Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Food Science and Nutrition, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Famenity Co., Ltd., Uiwang, Gyeonggi 16006, Republic of Korea
    Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 183
    |
    Published online on: January 7, 2021
       https://doi.org/10.3892/etm.2021.9614
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Abstract

Erigeron annuus (L.) PERS. (EALP) and Clematis mandshurica RUPR. (CMR) have been used in traditional remedies due to their medicinal effects. Recently, we reported that pretreatment with 200 mg/kg of YES‑10® (a combination of extracts from leaves of EALP and CMR) displayed neuroprotective effects against brain ischemia and reperfusion injury. The present study analyzed the major ingredients of YES‑10® and investigated whether neuroprotection from YES‑10® was dependent upon antioxidant effects in the cornu ammonis 1 (CA1) field in the gerbil hippocampus, after transient forebrain ischemia for 5 min. YES‑10® was demonstrated to predominantly contain scutellarin and chlorogenic acid. Pretreatment with YES‑10® significantly increased protein levels and the immunoreactivity of copper/zinc‑superoxide dismutase (SOD1) and manganese‑superoxide dismutase (SOD2) was in the pyramidal neurons of the hippocampal CA1 field when these were examined prior to transient ischemia induction. The increased SODs in CA1 pyramidal neurons following YES‑10® treatment were maintained after ischemic injury. In this case, the CA1 pyramidal neurons were protected from ischemia‑reperfusion injury. Oxidative stress was significantly attenuated in the CA1 pyramidal neurons, and this was determined by 4‑hydroxy‑2‑nonenal immunohistochemistry and dihydroethidium histofluorescence staining. Taken together, the results indicated that YES‑10® significantly attenuated transient ischemia‑induced oxidative stress and may be utilized for developing a protective agent against ischemic insults.
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Park Y, Noh Y, Kim D, Lee T, Ahn J, Kim B, Lee J, Park C, Park J, Kim J, Kim J, et al: Experimental pretreatment with YES‑10<sup>®</sup>, a plant extract rich in scutellarin and chlorogenic acid, protects hippocampal neurons from ischemia/reperfusion injury via antioxidant role. Exp Ther Med 21: 183, 2021.
APA
Park, Y., Noh, Y., Kim, D., Lee, T., Ahn, J., Kim, B. ... Won, M. (2021). Experimental pretreatment with YES‑10<sup>®</sup>, a plant extract rich in scutellarin and chlorogenic acid, protects hippocampal neurons from ischemia/reperfusion injury via antioxidant role. Experimental and Therapeutic Medicine, 21, 183. https://doi.org/10.3892/etm.2021.9614
MLA
Park, Y., Noh, Y., Kim, D., Lee, T., Ahn, J., Kim, B., Lee, J., Park, C., Park, J., Kim, J., Kim, Y., Kang, I., Lee, J., Kim, S., Won, M."Experimental pretreatment with YES‑10<sup>®</sup>, a plant extract rich in scutellarin and chlorogenic acid, protects hippocampal neurons from ischemia/reperfusion injury via antioxidant role". Experimental and Therapeutic Medicine 21.3 (2021): 183.
Chicago
Park, Y., Noh, Y., Kim, D., Lee, T., Ahn, J., Kim, B., Lee, J., Park, C., Park, J., Kim, J., Kim, Y., Kang, I., Lee, J., Kim, S., Won, M."Experimental pretreatment with YES‑10<sup>®</sup>, a plant extract rich in scutellarin and chlorogenic acid, protects hippocampal neurons from ischemia/reperfusion injury via antioxidant role". Experimental and Therapeutic Medicine 21, no. 3 (2021): 183. https://doi.org/10.3892/etm.2021.9614
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Spandidos Publications style
Park Y, Noh Y, Kim D, Lee T, Ahn J, Kim B, Lee J, Park C, Park J, Kim J, Kim J, et al: Experimental pretreatment with YES‑10<sup>®</sup>, a plant extract rich in scutellarin and chlorogenic acid, protects hippocampal neurons from ischemia/reperfusion injury via antioxidant role. Exp Ther Med 21: 183, 2021.
APA
Park, Y., Noh, Y., Kim, D., Lee, T., Ahn, J., Kim, B. ... Won, M. (2021). Experimental pretreatment with YES‑10<sup>®</sup>, a plant extract rich in scutellarin and chlorogenic acid, protects hippocampal neurons from ischemia/reperfusion injury via antioxidant role. Experimental and Therapeutic Medicine, 21, 183. https://doi.org/10.3892/etm.2021.9614
MLA
Park, Y., Noh, Y., Kim, D., Lee, T., Ahn, J., Kim, B., Lee, J., Park, C., Park, J., Kim, J., Kim, Y., Kang, I., Lee, J., Kim, S., Won, M."Experimental pretreatment with YES‑10<sup>®</sup>, a plant extract rich in scutellarin and chlorogenic acid, protects hippocampal neurons from ischemia/reperfusion injury via antioxidant role". Experimental and Therapeutic Medicine 21.3 (2021): 183.
Chicago
Park, Y., Noh, Y., Kim, D., Lee, T., Ahn, J., Kim, B., Lee, J., Park, C., Park, J., Kim, J., Kim, Y., Kang, I., Lee, J., Kim, S., Won, M."Experimental pretreatment with YES‑10<sup>®</sup>, a plant extract rich in scutellarin and chlorogenic acid, protects hippocampal neurons from ischemia/reperfusion injury via antioxidant role". Experimental and Therapeutic Medicine 21, no. 3 (2021): 183. https://doi.org/10.3892/etm.2021.9614
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