LMTK2 regulates inflammation in lipopolysaccharide‑stimulated BV2 cells

  • Authors:
    • Qianyun Rui
    • Shugang Cao
    • Xiaozhu Wang
    • Xiaoyu Duan
    • Xinyi  Iao
    • Wanli Dong
    • Qi Fang
    • Xueguang Zhang
    • Qun Xue
  • View Affiliations

  • Published online on: January 18, 2021     https://doi.org/10.3892/etm.2021.9621
  • Article Number: 219
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Abstract

Microglia activation plays vital roles in neuroinflammatory pathologys. Lemurs tyrosine kinase 2 (LMTK2) was reported to regulate NF‑κB signals. In the present study, the roles of LMTK2 were investigated in lipopolysaccharide (LPS)‑treated BV‑2 cells. Reverse transcription‑quantitative (RT‑q)PCR and western blotting (WB) were utilized to analyze LMTK2 levels in LPS‑treated BV2 cells. MTT assay determined cell viabilities. Nitric oxide (NO) and prostaglandin E2 (PGE2) levels were assessed through Griess and enzyme‑linked immunosorbent assay (ELISA), respectively. The expression level of inducible NO synthase (iNOS) and cyclooxygenase‑2 (COX‑2) were detected through RT‑qPCR and WB. The release of inflammatory mediators under LPS stimulation, tumor necrosis factor‑α (TNF‑α), interleukin‑1β (IL‑1β), IL‑6 and IL‑10, were analyzed through ELISA. WB was used to analyze the nuclear factor erythroid 2‑related factor 2 (Nrf2)/heme oxygenase 1 (HO‑1)/NAD(P)H dehydrogenase quinone 1 (NQO1) signal pathway. The results showed that the levels of the inflammatory mediators, iNOS, NO, COX‑2 and PGE2, along with pro‑inflammatory factors, TNF‑α, IL‑1β and IL‑6, were significantly decreased following the induction of exogenous LMTK2 expression by LMTK2 overexpression plasmids in LPS‑induced BV2 microglia. In contrast, anti‑inflammatory factor IL‑10 showed obvious decrease. Additionally, LMTK2 overexpression induced the elevation of Nrf2 in the cytoplasm and nucleus, along with the upregulation of HO‑1 and NQO1 expression. In conclusion, LMTK2 is postulated to regulate neuroinflammation possibly through Nrf2 pathway. The present study is essential to reveal the underlying function of LMTK2 and to identify novel therapeutic targets for drug development in treating neuroinflammation.
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March-2021
Volume 21 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Rui Q, Cao S, Wang X, Duan X, Iao X, Dong W, Fang Q, Zhang X and Xue Q: LMTK2 regulates inflammation in lipopolysaccharide‑stimulated BV2 cells. Exp Ther Med 21: 219, 2021
APA
Rui, Q., Cao, S., Wang, X., Duan, X., Iao, X., Dong, W. ... Xue, Q. (2021). LMTK2 regulates inflammation in lipopolysaccharide‑stimulated BV2 cells. Experimental and Therapeutic Medicine, 21, 219. https://doi.org/10.3892/etm.2021.9621
MLA
Rui, Q., Cao, S., Wang, X., Duan, X., Iao, X., Dong, W., Fang, Q., Zhang, X., Xue, Q."LMTK2 regulates inflammation in lipopolysaccharide‑stimulated BV2 cells". Experimental and Therapeutic Medicine 21.3 (2021): 219.
Chicago
Rui, Q., Cao, S., Wang, X., Duan, X., Iao, X., Dong, W., Fang, Q., Zhang, X., Xue, Q."LMTK2 regulates inflammation in lipopolysaccharide‑stimulated BV2 cells". Experimental and Therapeutic Medicine 21, no. 3 (2021): 219. https://doi.org/10.3892/etm.2021.9621