Detection of microRNA‑33a‑5p in serum, urine and renal tissue of patients with IgA nephropathy

Liu,Lihua; Duan,Aosong; Guo,Qiaoyan; Sun,Guangdong; Cui,Wenpeng; Lu,Xuehong; Yu,Hongyu; Luo,Ping

doi:10.3892/etm.2021.9638

March-2021 Volume 21 Issue 3

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March-2021 Volume 21 Issue 3

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Detection of microRNA‑33a‑5p in serum, urine and renal tissue of patients with IgA nephropathy

Authors:
- Lihua Liu
- Aosong Duan
- Qiaoyan Guo
- Guangdong Sun
- Wenpeng Cui
- Xuehong Lu
- Hongyu Yu
- Ping Luo
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Affiliations: Department of Nephrology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Department of Intensive Care Unit, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China

Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 205
|
Published online on: January 11, 2021

https://doi.org/10.3892/etm.2021.9638
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Abstract

The present study aimed to detect the levels of microRNA (miR)‑33a‑5p in the renal tissue, serum and urine of patients with primary IgA nephropathy (IgAN), thereby preliminarily exploring the association between the levels of miR‑33a‑5p and the condition of primary IgAN to provide evidence for the expression of miR‑33a‑5p in the serum and urine of IgAN patients as a clinical marker. Reverse‑transcription quantitative PCR was performed to evaluate the level of miR‑33a‑5p in IgAN patients according to severity and pathological classification. The results suggested that the levels of miR‑33a‑5p in the serum, urine and kidney tissues of patients with IgAN were lower than those of the control tissues obtained from cancer patients (0.28±0.25 vs. 1.00±0.45, P<0.05; 0.34±0.28 vs. 1.00±0.53, P<0.05; 0.47±0.27 vs. 1.00±0.38, P<0.05, respectively). Receiver operating characteristic curve analysis suggested that the serum and urine levels of miR‑33a‑5p may be used as a marker to differentiate renal injury in IgAN patients from healthy individuals. At the same time, according to the estimated glomerular filtration rate (eGFR) and Lee classification of nephropathy, it was determined that with the progression of renal failure and the increase of the pathological grade of kidney tissue, the relative level of miR‑33a‑5p in kidney tissue also decreased (eGFR <50 ml/min vs. eGFR ≥50 ml/min/1.73 m² group: 0.38±0.27 vs. 1.00±0.34, P<0.001; Lee grade ≤3 group vs. Lee grade >3: 1.00±0.48 vs. 0.38±0.45, P<0.05). This result suggested that the levels of miR‑33a‑5p in serum, urine and kidney tissues decreased with the severity of renal injury and the progression of renal failure in patients with IgAN. Hence, miR‑33a‑5p detected in the serum and urine may be used as a non‑invasive biomarker to reflect the progression of renal injury and renal failure in patients with IgAN.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Detection of microRNA‑33a‑5p in serum, urine and renal tissue of patients with IgA nephropathy

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