Open Access

Quantitative T2 mapping of rats with chronic hepatitis

  • Authors:
    • Xiaofeng Wang
    • Yan Hu
    • Xin Lu
    • Yu Cai
    • Jian Shu
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  • Published online on: January 18, 2021     https://doi.org/10.3892/etm.2021.9656
  • Article Number: 225
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the study was to explore the diagnostic value of T2 mapping in an experimental rat model of chronic liver disease. Chronic hepatitis was induced in Sprague‑Dawley male rats (n=88) by intraperitoneal and abdominal subcutaneous injection of carbon tetrachloride in olive oil. The normal control rats (n=12) were similarly injected with the same dose of normal saline. All rats were randomly selected and subjected to T2‑weighted/spectral adiabatic inversion recovery and multiple gradient‑ and spin‑echo sequence. After scanning, rats were sacrificed immediately and livers removed for staining with hematoxylin and eosin, as well as Masson's trichrome, to determine the pathological stage of hepatic fibrosis, necroinflammatory activity and steatosis. The T2 values were measured and associated with histopathological findings. The T2 values were significantly associated with hepatic fibrosis (P<0.05), but not with hepatitis (P>0.05) or steatosis (P>0.05). By partial correlation analysis, a significant positive correlation was observed between the T2 values and stages of liver fibrosis (r=0.820; P<0.05). T2 values increased with progressive hepatic fibrosis. The differences between T2 values and stages of liver fibrosis were statistically significant. Statistically significant differences were observed between different stages of liver fibrosis (P<0.05), with an area under the curve value of 0.944 for predicting stage F1 or greater, 0.942 for stage F2 or greater, 0.958 for stage F3 or greater, and 0.948 for F4. Thus, the T2 value is one of the quantitative indices of imaging and accurately reflects the stages of liver fibrosis.
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March-2021
Volume 21 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang X, Hu Y, Lu X, Cai Y and Shu J: Quantitative T2 mapping of rats with chronic hepatitis. Exp Ther Med 21: 225, 2021
APA
Wang, X., Hu, Y., Lu, X., Cai, Y., & Shu, J. (2021). Quantitative T2 mapping of rats with chronic hepatitis. Experimental and Therapeutic Medicine, 21, 225. https://doi.org/10.3892/etm.2021.9656
MLA
Wang, X., Hu, Y., Lu, X., Cai, Y., Shu, J."Quantitative T2 mapping of rats with chronic hepatitis". Experimental and Therapeutic Medicine 21.3 (2021): 225.
Chicago
Wang, X., Hu, Y., Lu, X., Cai, Y., Shu, J."Quantitative T2 mapping of rats with chronic hepatitis". Experimental and Therapeutic Medicine 21, no. 3 (2021): 225. https://doi.org/10.3892/etm.2021.9656