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Article

Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide

  • Authors:
    • Xue Li
    • Xiaoxuan Wang
    • Qing-Xian Luan
  • View Affiliations / Copyright

    Affiliations: Department of Periodontology, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing 100081, P.R. China
  • Article Number: 417
    |
    Published online on: February 25, 2021
       https://doi.org/10.3892/etm.2021.9861
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Abstract

The present study aimed to investigate whether gingival fibroblasts (GFs) of patients with aggressive periodontitis (AgP) are more sensitive to lipopolysaccharide (LPS) stimulation than GFs of control subjects. AgP causes rapid periodontal destruction, including the production of cytokines [i.e. interleukin (IL)‑1β, IL‑6 and tumor necrosis factor (TNF)‑α] and matrix metalloproteinases (MMP)‑1, ‑3 and ‑9 in AgP GFs. LPS upregulates IL‑1β, IL‑6, TNF‑α, MMP‑1, MMP‑3, MMP‑9 and mitochondrial reactive oxygen species (mtROS). Fibroblasts are known to be associated with immune responses to bacterial virulence factors, but the precise mechanisms underlying this severe periodontal disease are unclear. In the present study, primary human GFs of four patients with AgP and four healthy subjects were challenged in vitro with LPS from Porphyromonas gingivalis (P. gingivalis). The generation of mtROS in GFs was assessed using MitoSOX Red. The expression of genes encoding inflammatory cytokines and MMPs in GFs was analyzed using reverse transcription‑quantitative polymerase chain reaction, and the expression of proteins was analyzed using ELISA and Western blotting. Human GFs of patients with AgP exhibited higher levels of mtROS, and higher mRNA and protein expression levels of proinflammatory cytokines, including IL‑1β, IL‑6, MMP‑1, MMP‑3 and MMP‑9 compared with healthy human GFs following stimulation with LPS from P. gingivalis. In the present study, it was demonstrated that GFs of patients with AgP display hyperreactivity when challenged with LPS.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Wang X and Luan Q: Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide. Exp Ther Med 21: 417, 2021.
APA
Li, X., Wang, X., & Luan, Q. (2021). Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide. Experimental and Therapeutic Medicine, 21, 417. https://doi.org/10.3892/etm.2021.9861
MLA
Li, X., Wang, X., Luan, Q."Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide". Experimental and Therapeutic Medicine 21.5 (2021): 417.
Chicago
Li, X., Wang, X., Luan, Q."Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide". Experimental and Therapeutic Medicine 21, no. 5 (2021): 417. https://doi.org/10.3892/etm.2021.9861
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Wang X and Luan Q: Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide. Exp Ther Med 21: 417, 2021.
APA
Li, X., Wang, X., & Luan, Q. (2021). Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide. Experimental and Therapeutic Medicine, 21, 417. https://doi.org/10.3892/etm.2021.9861
MLA
Li, X., Wang, X., Luan, Q."Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide". Experimental and Therapeutic Medicine 21.5 (2021): 417.
Chicago
Li, X., Wang, X., Luan, Q."Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide". Experimental and Therapeutic Medicine 21, no. 5 (2021): 417. https://doi.org/10.3892/etm.2021.9861
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