Hydrogen gas alleviates acute alcohol‑induced liver injury by inhibiting JNK activation

Zhang,Yaxing; Bi,Mingmin; Chen,Zifeng; Dai,Min; Zhou,Ge; Hu,Yuxuan; Yang,Hongzhi; Guan,Weibing

doi:10.3892/etm.2021.9884

Hydrogen gas alleviates acute alcohol‑induced liver injury by inhibiting JNK activation

Authors:
- Yaxing Zhang
- Mingmin Bi
- Zifeng Chen
- Min Dai
- Ge Zhou
- Yuxuan Hu
- Hongzhi Yang
- Weibing Guan
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Affiliations: Department of Traditional Chinese Medicine, The Third Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510630, P.R. China, Department of Otorhinolaryngology, The Seventh Affiliated Hospital, Sun Yat‑sen University, Shenzhen, Guangdong 518107, P.R. China, Biofeedback Laboratory, Xinhua College of Sun Yat‑sen University, Guangzhou, Guangdong 510520, P.R. China
Published online on: March 1, 2021 https://doi.org/10.3892/etm.2021.9884
Article Number: 453
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Binge alcohol drinking is fast becoming a global health concern, with the liver among the first organ involved and the one afflicted with the greatest degree of injury. Oxidative stress, alterations in hepatic metabolism, immunity and inflammation have all been reported to contribute to the development of alcoholic liver disease (ALD). Hydrogen gas (H₂) serves a key role in the modulation of hepatic redox, immune and inflammatory homeostasis. However, the effects of treatment using intraperitoneal injection of H₂ on ALD remain unexplored. Therefore, the aim of the present study was to investigate the effects and underlying mechanism of intraperitoneal injection of H₂ on acute alcohol‑induced liver injury in a mouse model. H₂ was administered by daily intraperitoneal injections (1.0 ml/100 g) for 4 days. On day 4, the mice received H₂ after fasting for 5.5 h. After 30 min, the mice were administered with 33% (v/v) ethanol at a cumulative dose of 4.5 g/kg body weight by four equally divided gavages at 20‑min intervals. Blood and liver tissues were collected at 16 h after the first ethanol gavage. Subsequently, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride and total cholesterol (TC) levels were analyzed using an Automatic Clinical Analyzer. Hepatic JNK activity and GAPDH levels were examined by western blotting. It was observed that acute ethanol gavage induced liver injury, as indicated by significantly increased serum ALT and AST levels, which were effectively decreased by H₂ at 16 h after the first ethanol gavage. In addition, H₂ treatment reduced serum TC levels in the Alcohol+H₂ group when compared with those in Alcohol group. Mechanistically, H₂ attenuated hepatic JNK phosphorylation induced by acute ethanol gavage. Therefore, the results of the present study demonstrated that treatment with exogenous H₂ by intraperitoneal injection may alleviate acute alcohol‑induced liver injury by inhibiting hepatic JNK activation, which may represent a novel therapeutic strategy for ALD.

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