Protective effects of gastrodin pretreatment on mouse hepatic ischemia‑reperfusion occurring through antioxidant and anti‑apoptotic mechanisms

  • Authors:
    • Bo Sun
    • Jie Jiang
    • Xinyan Zhu
    • Dan Yang
    • Zhenyu Cui
    • Yu Zhang
    • Minbo Zhang
    • Yiting Qian
    • Ruilin Liu
    • Wenzhuo Yang
  • View Affiliations

  • Published online on: March 8, 2021     https://doi.org/10.3892/etm.2021.9902
  • Article Number: 471
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Abstract

Hepatic ischemia‑reperfusion injury (HIRI) often occurs following surgical procedures such as liver resection and transplantation. However, despite its clinical prominence, to the best of our knowledge, there remain no effective strategies to treat HIRI. Therefore, the aim of present study was to identify therapeutic agents that can exert beneficial effects against HIRI. The present study found that following hepatic IR modeling in mice, gastrodin (Gas) pretreatment improved the IR outcomes in terms of the serum biochemical indexes (alanine transaminase and aspartate transaminase), tissue biochemical indexes (superoxide dismutase, malondialdehyde and reduced glutathione content) and tissue pathology (H&E staining). In addition, compared with those in the IR + vehicle group, the IR + Gas group showed upregulated expression levels of nuclear erythroid 2‑related factor 2, heme oxygenase 1 and Bcl‑2 as detected by western blotting and reverse transcription‑quantitative PCR. The mRNA and protein expression levels of Bax and caspase‑3 were downregulated in the IR + Gas group compared with the IR + vehicle group. Concurrently, no significant differences were observed in the parameters between the Sham + vehicle and the Sham + Gas groups, indicating that Gas pretreatment may not cause liver damage. In conclusion, the findings of the present study revealed that Gas pretreatment exerted a protective effect in HIRI through both antioxidant and anti‑apoptotic mechanisms.
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May-2021
Volume 21 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Sun B, Jiang J, Zhu X, Yang D, Cui Z, Zhang Y, Zhang M, Qian Y, Liu R, Yang W, Yang W, et al: Protective effects of gastrodin pretreatment on mouse hepatic ischemia‑reperfusion occurring through antioxidant and anti‑apoptotic mechanisms. Exp Ther Med 21: 471, 2021
APA
Sun, B., Jiang, J., Zhu, X., Yang, D., Cui, Z., Zhang, Y. ... Yang, W. (2021). Protective effects of gastrodin pretreatment on mouse hepatic ischemia‑reperfusion occurring through antioxidant and anti‑apoptotic mechanisms. Experimental and Therapeutic Medicine, 21, 471. https://doi.org/10.3892/etm.2021.9902
MLA
Sun, B., Jiang, J., Zhu, X., Yang, D., Cui, Z., Zhang, Y., Zhang, M., Qian, Y., Liu, R., Yang, W."Protective effects of gastrodin pretreatment on mouse hepatic ischemia‑reperfusion occurring through antioxidant and anti‑apoptotic mechanisms". Experimental and Therapeutic Medicine 21.5 (2021): 471.
Chicago
Sun, B., Jiang, J., Zhu, X., Yang, D., Cui, Z., Zhang, Y., Zhang, M., Qian, Y., Liu, R., Yang, W."Protective effects of gastrodin pretreatment on mouse hepatic ischemia‑reperfusion occurring through antioxidant and anti‑apoptotic mechanisms". Experimental and Therapeutic Medicine 21, no. 5 (2021): 471. https://doi.org/10.3892/etm.2021.9902