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Klotho overexpression suppresses apoptosis by regulating the Hsp70/Akt/Bad pathway in H9c2(2-1) cells

  • Authors:
    • Jinpeng Hu
    • Bin Su
    • Xuewen Li
    • Yuming Li
    • Jihong Zhao
  • View Affiliations / Copyright

    Affiliations: Graduate School of Tianjin Medical University, Tianjin 300070, P.R. China, Ministry of Research, Characteristic Medical Center of The Chinese People's Armed Police Force, Tianjin 300162, P.R. China, Department of Geriatric Medicine, Characteristic Medical Center of Chinese People's Armed Police Force, Tianjin 300162, P.R. China, Angiocardiopathy Institute of Characteristic Medical Center of PAP, Tianjin 300162, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 486
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    Published online on: March 16, 2021
       https://doi.org/10.3892/etm.2021.9917
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Abstract

Early reperfusion is the most effective and important treatment for acute myocardial infarction. However, reperfusion therapy often leads to a certain degree of myocardial damage. The aim of the present study was to identify the role of klotho, and the molecular mechanism underlying its effects, in myocardial damage using a model of myocardial hypoxia injury. Hypoxia/reoxygenation (H/R) was used to mimic ischemia/reperfusion (I/R) injury in vitro. The expression and distribution of klotho in H9c2(2‑1) cells was observed by fluorogenic scanning, and the apoptotic rate was determined by Annexin V‑FITC/propidium iodide dual staining. Cell viability was determined by MTT assay, and caspase‑3, cleaved caspase‑3, Bcl‑2, Bax, heat shock protein (Hsp) 70 and Akt levels were assessed by western blotting. A lactate dehydrogenase test was performed to determine the degree of H9c2(2‑1) cell damage. The results revealed that klotho was primarily located in the cytoplasm of H9c2(2‑1) cells. Klotho overexpression markedly suppressed H/R‑induced H9c2(2‑1) cell apoptosis. Furthermore, cell viability increased, and injury decreased in response to klotho. Klotho also suppressed the activation of caspase‑3, upregulated Bcl2 and decreased Bax levels following H/R injury, as well as alleviating H/R injury by upregulating the expression of Hsp70 and increasing the levels of phosphorylated (p‑)Akt and Bad. In conclusion, these results indicate that klotho suppressed H/R‑induced H9c2(2‑1) cell apoptosis by regulating the levels of Hsp70, p‑Akt and p‑Bad, which suggest that klotho could be a novel agent for the treatment of coronary disease.
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1 

Alabas OA, Jernberg T, Pujades-Rodriguez M, Rutherford MJ, West RM, Hall M, Timmis A, Lindahl B, Fox KA, Hemingway H, et al: Statistics on mortality following acute myocardial infarction in 842 897 Europeans. Cardiovasc Res. 116:149–157. 2020.PubMed/NCBI View Article : Google Scholar

2 

Ioacara S, Popescu AC, Tenenbaum J, Dimulescu DR, Popescu MR, Sirbu A and Fica S: Acute Myocardial Infarction Mortality Rates and Trends in Romania between 1994 and 2017. Int J Environ Res Public Health. 17(285)2019.PubMed/NCBI View Article : Google Scholar

3 

Neri M, Riezzo I, Pascale N, Pomara C and Turillazzi E: Ischemia/reperfusion injury following acute myocardial infarction: A critical issue for clinicians and forensic pathologists. Mediators Inflamm. 2017(7018393)2017.PubMed/NCBI View Article : Google Scholar

4 

Wang M and Wan J: Soluble regulators of Interleukin-1 signaling: Novel biomarkers for early acute myocardial infarction diagnosis and to predict ischemia/reperfusion injury? Int J Cardiol. 274(357)2019.PubMed/NCBI View Article : Google Scholar

5 

Feng M, Wang Q, Wang H and Guan W: Tumor necrosis factor-alpha preconditioning attenuates liver ischemia/reperfusion injury through preserving sarco/endoplasmic reticulum calcium-ATPase function. J Surg Res. 184:1109–1113. 2013.PubMed/NCBI View Article : Google Scholar

6 

Li X, Dai Y, Yan S, Shi Y, Han B, Li J, Cha L and Mu J: Down-regulation of lncRNA KCNQ1OT1 protects against myocardial ischemia/reperfusion injury following acute myocardial infarction. Biochem Biophys Res Commun. 491:1026–1033. 2017.PubMed/NCBI View Article : Google Scholar

7 

Tu CC, Wan BY and Zeng Y: STIM2 knockdown protects against ischemia/reperfusion injury through reducing mitochondrial calcium overload and preserving mitochondrial function. Life Sci. 247(116560)2020.PubMed/NCBI View Article : Google Scholar

8 

Yue R, Xia X, Jiang J, Yang D, Han Y, Chen X, Cai Y, Li L, Wang WE and Zeng C: Mitochondrial DNA oxidative damage contributes to cardiomyocyte ischemia/reperfusion-injury in rats: Cardioprotective role of lycopene. J Cell Physiol. 230:2128–2141. 2015.PubMed/NCBI View Article : Google Scholar

9 

Wang Z, Sun R, Wang G, Chen Z, Li Y, Zhao Y, Liu D, Zhao H, Zhang F, Yao J, et al: SIRT3-mediated deacetylation of PRDX3 alleviates mitochondrial oxidative damage and apoptosis induced by intestinal ischemia/reperfusion injury. Redox Biol. 28(101343)2020.PubMed/NCBI View Article : Google Scholar

10 

Wang X, He F, Liao Y, Song X, Zhang M, Qu L, Luo T, Zhou S, Ling Y, Guo J, et al: Baicalin pretreatment protects against myocardial ischemia/reperfusion injury by inhibiting mitochondrial damage-mediated apoptosis. Int J Cardiol. 168:4343–4345. 2013.PubMed/NCBI View Article : Google Scholar

11 

Yang Y, Duan W, Jin Z, Yi W, Yan J, Zhang S, Wang N, Liang Z, Li Y, Chen W, et al: JAK2/STAT3 activation by melatonin attenuates the mitochondrial oxidative damage induced by myocardial ischemia/reperfusion injury. J Pineal Res. 55:275–286. 2013.PubMed/NCBI View Article : Google Scholar

12 

Xie Y, Jiang D, Xiao J, Fu C, Zhang Z, Ye Z and Zhang X: Ischemic preconditioning attenuates ischemia/reperfusion-induced kidney injury by activating autophagy via the SGK1 signaling pathway. Cell Death Dis. 9(338)2018.PubMed/NCBI View Article : Google Scholar

13 

Zhou LY, Zhai M, Huang Y, Xu S, An T, Wang YH, Zhang RC, Liu CY, Dong YH, Wang M, et al: The circular RNA ACR attenuates myocardial ischemia/reperfusion injury by suppressing autophagy via modulation of the Pink1/ FAM65B pathway. Cell Death Differ. 26:1299–1315. 2019.PubMed/NCBI View Article : Google Scholar

14 

Di Bona D, Accardi G, Virruso C, Candore G and Caruso C: Association of Klotho polymorphisms with healthy aging: A systematic review and meta-analysis. Rejuvenation Res. 17:212–216. 2014.PubMed/NCBI View Article : Google Scholar

15 

Wang Q, Su W, Shen Z and Wang R: Correlation between soluble alpha-Klotho and renal function in patients with chronic kidney disease: A Review and meta-analysis. Biomed Res Int. 2018(9481475)2018.PubMed/NCBI View Article : Google Scholar

16 

Mosa O, Skitek M and Jerin A: Validity of Klotho, CYR61 and YKL-40 as ideal predictive biomarkers for acute kidney injury: Review study. Sao Paulo Med J. 135:57–65. 2017.PubMed/NCBI View Article : Google Scholar

17 

Kuwahara N, Sasaki S, Kobara M, Nakata T, Tatsumi T, Irie H, Narumiya H, Hatta T, Takeda K, Matsubara H, et al: HMG-CoA reductase inhibition improves anti-aging klotho protein expression and arteriosclerosis in rats with chronic inhibition of nitric oxide synthesis. Int J Cardiol. 123:84–90. 2008.PubMed/NCBI View Article : Google Scholar

18 

Yang K, Wang C, Nie L, Zhao X, Gu J, Guan X, Wang S, Xiao T, Xu X, He T, et al: Klotho protects against indoxyl sulphate-induced myocardial hypertrophy. J Am Soc Nephrol. 26:2434–2446. 2015.PubMed/NCBI View Article : Google Scholar

19 

Ramez M, Rajabi H, Ramezani F, Naderi N, Darbandi-Azar A and Nasirinezhad F: The greater effect of high-intensity interval training versus moderate-intensity continuous training on cardioprotection against ischemia-reperfusion injury through Klotho levels and attenuate of myocardial TRPC6 expression. BMC Cardiovasc Disord. 19(118)2019.PubMed/NCBI View Article : Google Scholar

20 

Takenaka T, Kobori H, Miyazaki T, Suzuki H, Nishiyama A, Ishii N, Yamashita M and Hayashi M: Klotho protein supplementation reduces blood pressure and renal hypertrophy in db/db mice, a model of type 2 diabetes. Acta Physiol (Oxf). 225(e13190)2019.PubMed/NCBI View Article : Google Scholar

21 

Donate-Correa J, Martín-Núñez E, Delgado NP, de Fuentes MM, Arduan AO, Mora-Fernández C and Navarro González JF: Implications of Fibroblast growth factor/Klotho system in glucose metabolism and diabetes. Cytokine Growth Factor Rev. 28:71–77. 2016.PubMed/NCBI View Article : Google Scholar

22 

Fountoulakis N, Maltese G, Gnudi L and Karalliedde J: Reduced levels of anti-ageing hormone Klotho predict renal function decline in type 2 diabetes. J Clin Endocrinol Metab. 103:2026–2032. 2018.PubMed/NCBI View Article : Google Scholar

23 

Zhou X and Wang X: Klotho: A novel biomarker for cancer. J Cancer Res Clin Oncol. 141:961–969. 2015.PubMed/NCBI View Article : Google Scholar

24 

Fakhar M, Najumuddin Gul M and Rashid S: Antagonistic role of Klotho-derived peptides dynamics in the pancreatic cancer treatment through obstructing WNT-1 and Frizzled binding. Biophys Chem. 240:107–117. 2018.PubMed/NCBI View Article : Google Scholar

25 

Li Q, Li Y, Liang L, Li J, Luo D, Liu Q, Cai S and Li X: Klotho negatively regulated aerobic glycolysis in colorectal cancer via ERK/HIF1alpha axis. Cell Commun Signal. 16(26)2018.PubMed/NCBI View Article : Google Scholar

26 

Pako J, Bikov A, Barta I, Matsueda H, Puskas R, Galffy G, Kerpel-Fronius A, Antus B and Horvath I: Assessment of the circulating klotho protein in lung cancer patients. Pathol Oncol Res. 26:233–238. 2020.PubMed/NCBI View Article : Google Scholar

27 

Wang N, Ma J, Ren Y, Xiang S and Jia R: Secreted klotho from exosomes alleviates inflammation and apoptosis in acute pancreatitis. Am J Transl Res. 11:3375–3383. 2019.PubMed/NCBI

28 

Sugiura H, Yoshida T, Mitobe M, Yoshida S, Shiohira S, Nitta K and Tsuchiya K: Klotho reduces apoptosis in experimental ischaemic acute kidney injury via HSP-70. Nephrol Dial Transplant. 25:60–68. 2010.PubMed/NCBI View Article : Google Scholar

29 

Kim SJ, Cheresh P, Eren M, Jablonski RP, Yeldandi A, Ridge KM, Budinger GR, Kim DH, Wolf M, Vaughan DE, et al: Klotho, an antiaging molecule, attenuates oxidant-induced alveolar epithelial cell mtDNA damage and apoptosis. Am J Physiol Lung Cell Mol Physiol. 313:L16–L26. 2017.PubMed/NCBI View Article : Google Scholar

30 

Zhu H, Gao Y, Zhu S, Cui Q and Du J: Klotho Improves cardiac function by suppressing reactive oxygen species (ROS) mediated apoptosis by modulating Mapks/Nrf2 signaling in doxorubicin-induced cardiotoxicity. Med Sci Monit. 23:5283–5293. 2017.PubMed/NCBI View Article : Google Scholar

31 

Song S, Gao P, Xiao H, Xu Y and Si LY: Klotho suppresses cardiomyocyte apoptosis in mice with stress-induced cardiac injury via downregulation of endoplasmic reticulum stress. PLoS One. 8(e82968)2013.PubMed/NCBI View Article : Google Scholar

32 

Liang X, Li B, Huang Q, Liu D and Ma H: Klotho prevents DEX-induced apoptosis in MC3T3-E1 osteoblasts through the NF-κB signaling pathway. Biochem Biophys Res Commun. 507:355–361. 2018.PubMed/NCBI View Article : Google Scholar

33 

Cui W, Leng B, Liu W and Wang G: Suppression of apoptosis in human umbilical vein endothelial cells (HUVECs) by Klotho protein is associated with reduced endoplasmic reticulum oxidative stress and activation of the PI3K/AKT pathway. Med Sci Monit. 24:8489–8499. 2018.PubMed/NCBI View Article : Google Scholar

34 

Aijaz S, Ahmed N, Akhter Z, Sattar S, Lakhani S, Malik R and Pathan A: Clinical characteristics and in-hospital outcome in percutaneous coronary interventions with ST elevation myocardial infarction patients developing acute kidney injury. J Pak Med Assoc. 69:1827–1833. 2019.PubMed/NCBI View Article : Google Scholar

35 

Wang C, Pei YY, Ma YH, Ma XL, Liu ZW and Zhu JH: Risk factors for acute kidney injury in patients with acute myocardial infarction. Chin Med J (Engl). 132:1660–1665. 2019.PubMed/NCBI View Article : Google Scholar

36 

Wang DX, Huang Z, Li QJ, Zhong GQ, He Y, Huang WQ, Cao XL, Tu RH and Meng JJ: Involvement of HSP90 in ischemic postconditioning-induced cardioprotection by inhibition of the complement system, JNK and inflammation. Acta Cir Bras. 35(e202000105)2020.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Hu J, Su B, Li X, Li Y and Zhao J: Klotho overexpression suppresses apoptosis by regulating the Hsp70/Akt/Bad pathway in H9c2(2-1) cells. Exp Ther Med 21: 486, 2021.
APA
Hu, J., Su, B., Li, X., Li, Y., & Zhao, J. (2021). Klotho overexpression suppresses apoptosis by regulating the Hsp70/Akt/Bad pathway in H9c2(2-1) cells. Experimental and Therapeutic Medicine, 21, 486. https://doi.org/10.3892/etm.2021.9917
MLA
Hu, J., Su, B., Li, X., Li, Y., Zhao, J."Klotho overexpression suppresses apoptosis by regulating the Hsp70/Akt/Bad pathway in H9c2(2-1) cells". Experimental and Therapeutic Medicine 21.5 (2021): 486.
Chicago
Hu, J., Su, B., Li, X., Li, Y., Zhao, J."Klotho overexpression suppresses apoptosis by regulating the Hsp70/Akt/Bad pathway in H9c2(2-1) cells". Experimental and Therapeutic Medicine 21, no. 5 (2021): 486. https://doi.org/10.3892/etm.2021.9917
Copy and paste a formatted citation
x
Spandidos Publications style
Hu J, Su B, Li X, Li Y and Zhao J: Klotho overexpression suppresses apoptosis by regulating the Hsp70/Akt/Bad pathway in H9c2(2-1) cells. Exp Ther Med 21: 486, 2021.
APA
Hu, J., Su, B., Li, X., Li, Y., & Zhao, J. (2021). Klotho overexpression suppresses apoptosis by regulating the Hsp70/Akt/Bad pathway in H9c2(2-1) cells. Experimental and Therapeutic Medicine, 21, 486. https://doi.org/10.3892/etm.2021.9917
MLA
Hu, J., Su, B., Li, X., Li, Y., Zhao, J."Klotho overexpression suppresses apoptosis by regulating the Hsp70/Akt/Bad pathway in H9c2(2-1) cells". Experimental and Therapeutic Medicine 21.5 (2021): 486.
Chicago
Hu, J., Su, B., Li, X., Li, Y., Zhao, J."Klotho overexpression suppresses apoptosis by regulating the Hsp70/Akt/Bad pathway in H9c2(2-1) cells". Experimental and Therapeutic Medicine 21, no. 5 (2021): 486. https://doi.org/10.3892/etm.2021.9917
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