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Article

Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients

  • Authors:
    • Li Meng
    • Long Jiang
    • Jian Chen
    • Hongjin Ren
    • Zhiqin Gao
    • Fei Wu
    • Yiyang Wen
    • Lianjuan Yang
  • View Affiliations / Copyright

    Affiliations: Department of Dermatological Mycology, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443, P.R. China, Department of Dermatological Surgery, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443, P.R. China, Department of Dermatological Pathology, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443, P.R. China
  • Article Number: 489
    |
    Published online on: March 16, 2021
       https://doi.org/10.3892/etm.2021.9920
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Abstract

Atopic dermatitis (AD) is a common chronic relapsing inflammatory disease. There is substantial evidence suggesting that noncoding RNAs have indispensable roles in the pathogenesis of AD. Exosomal transfer RNA‑derived fragments (tRFs) have been identified as potential biomarkers for various disorders. However, the role of tRFs in AD has remained to be elucidated, which was thus the aim of the present study. Plasma samples from 23 pediatric patients with AD and 23 healthy controls were collected. Exosomes were successfully isolated from plasma according to the manufacturer's protocol. Small RNA sequencing was performed in 3 patients with AD and 3 controls, and 135 significantly differentially expressed plasma exosomal tRFs were identified, including 36 upregulated and 99 downregulated tRFs. Using the miRanda and RNAhybrid databases, 58,227 target genes of these 135 differentially expressed tRFs were predicted. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses suggested that these target genes of tRFs are involved in multiple functions and pathways associated with AD. Downregulation of tRF‑28‑QSZ34KRQ590K and tRF‑33‑Q99P9P9NH57SD3 were validated in 20 patients with AD and 20 controls by reverse transcription‑quantitative PCR and tRF‑28‑QSZ34KRQ590K exhibited significance in the receiver operating characteristic curve analysis. The present study was the first to provide a tRF profile in AD and implied that plasma exosomal tRF‑28‑QSZ34KRQ590K may be a potential biomarker for pediatric patients with AD. The present study enhanced the understanding of the pathogenesis of AD and provided a novel marker for the diagnosis and targeted treatment of AD.
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Copy and paste a formatted citation
Spandidos Publications style
Meng L, Jiang L, Chen J, Ren H, Gao Z, Wu F, Wen Y and Yang L: Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients. Exp Ther Med 21: 489, 2021.
APA
Meng, L., Jiang, L., Chen, J., Ren, H., Gao, Z., Wu, F. ... Yang, L. (2021). Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients. Experimental and Therapeutic Medicine, 21, 489. https://doi.org/10.3892/etm.2021.9920
MLA
Meng, L., Jiang, L., Chen, J., Ren, H., Gao, Z., Wu, F., Wen, Y., Yang, L."Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients". Experimental and Therapeutic Medicine 21.5 (2021): 489.
Chicago
Meng, L., Jiang, L., Chen, J., Ren, H., Gao, Z., Wu, F., Wen, Y., Yang, L."Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients". Experimental and Therapeutic Medicine 21, no. 5 (2021): 489. https://doi.org/10.3892/etm.2021.9920
Copy and paste a formatted citation
x
Spandidos Publications style
Meng L, Jiang L, Chen J, Ren H, Gao Z, Wu F, Wen Y and Yang L: Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients. Exp Ther Med 21: 489, 2021.
APA
Meng, L., Jiang, L., Chen, J., Ren, H., Gao, Z., Wu, F. ... Yang, L. (2021). Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients. Experimental and Therapeutic Medicine, 21, 489. https://doi.org/10.3892/etm.2021.9920
MLA
Meng, L., Jiang, L., Chen, J., Ren, H., Gao, Z., Wu, F., Wen, Y., Yang, L."Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients". Experimental and Therapeutic Medicine 21.5 (2021): 489.
Chicago
Meng, L., Jiang, L., Chen, J., Ren, H., Gao, Z., Wu, F., Wen, Y., Yang, L."Transfer RNA‑derived fragment tRF‑28‑QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients". Experimental and Therapeutic Medicine 21, no. 5 (2021): 489. https://doi.org/10.3892/etm.2021.9920
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