Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
May-2021 Volume 21 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2021 Volume 21 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Caffeine improves bladder function in diabetic rats via a neuroprotective effect

  • Authors:
    • Jun Xue
    • Yadong Liu
    • Sichong Zhang
    • Liucheng Ding
    • Baixin Shen
    • Yunpeng Shao
    • Zhongqing Wei
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210011, P.R. China
    Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 501
    |
    Published online on: March 17, 2021
       https://doi.org/10.3892/etm.2021.9932
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Diabetic cystopathy (DCP) is one of the most common complications of diabetes mellitus (DM). A previous study reported that caffeine may improve bladder dysfunction in rats with DM. The aim of the present study was to investigate the mechanisms behind the capacity for caffeine to improve bladder function in rats with DM. Sprague Dawley rats were divided into four groups: control, caffeine, DM and DM plus caffeine treatment (DM + caffeine). Bladder function was measured by urodynamic analyses. The levels of nerve growth factor (NGF), brain‑derived neurotrophic factor (BDNF) and calcitonin gene‑related peptide (CGRP) in the bladder tissue were detected by ELISA. Apoptosis in the dorsal root ganglion (DRG) was detected by terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling assay. The expression levels of B‑cell lymphoma‑2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), caspase‑3, cleaved caspase‑3, caspase‑9 and cleaved caspase‑9 proteins in the DRG were detected by western blotting. Following treatment with caffeine, the urination time and micturition interval of rats with DM were improved, the bladder wet weight was decreased, and the maximum voiding pressure was increased. Relative to that in the DM group, the expression levels of NGF, BDNF and CGRP in the bladder tissue of DM + caffeine rats increased; cellular apoptosis in the DRG of DM + caffeine rates decreased; and the expression levels of Bcl‑2, Bax, cleaved caspase‑3 and cleaved caspase‑9 proteins in the DRG of DM + caffeine rats were restored to a certain extent. In conclusion, caffeine promotes bladder function in rats with DM through a protective effect on DRG.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Xu Y, Wang L, He J, Bi Y, Li M, Wang T, Wang L, Jiang Y, Dai M, Lu J, et al: Prevalence and control of diabetes in Chinese adults. JAMA. 310:948–959. 2013.PubMed/NCBI View Article : Google Scholar

2 

Yuan Z, Tang Z, He C and Tang W: Diabetic cystopathy: A review. J Diabetes. 7:442–447. 2015.PubMed/NCBI View Article : Google Scholar

3 

Arrellano-Valdez F, Urrutia-Osorio M, Arroyo C and Soto-Vega E: A comprehensive review of urologic complications in patients with diabetes. Springerplus. 3(549)2014.PubMed/NCBI View Article : Google Scholar

4 

Feifer A and Corcos J: Contemporary role of suprapubic cystostomy in treatment of neuropathic bladder dysfunction in spinal cord injured patients. Neurourol Urodyn. 27:475–479. 2008.PubMed/NCBI View Article : Google Scholar

5 

Franz MJ, Bantle JP, Beebe CA, Brunzell JD, Chiasson JL, Garg A, Holzmeister LA, Hoogwerf B, Mayer-Davis E, Mooradian AD, et al: Evidence-based nutrition principles and recommendations for the treatment and prevention of diabetes and related complications. Diabetes Care. 26 (Suppl 1):S51–S61. 2003.PubMed/NCBI View Article : Google Scholar

6 

Cho YS, Ko IG, Kim SE, Hwan L, Shin MS, Kim CJ, Kim SH, Jin JJ, Chung JY and Kim KH: Caffeine enhances micturition through neuronal activation in micturition centers. Mol Med Rep. 10:2931–2936. 2014.PubMed/NCBI View Article : Google Scholar

7 

Yi CR, Wei ZQ, Deng XL, Sun ZY, Li XR and Tian CG: Effects of coffee and caffeine on bladder dysfunction in streptozotocin-induced diabetic rats. Acta Pharmacol Sin. 27:1037–1043. 2006.PubMed/NCBI View Article : Google Scholar

8 

Langdale CL, Thor KB, Marson L and Burgard EC: Maintenance of bladder innervation in diabetes: A stereological study of streptozotocin-treated female rats. Auton Neurosci. 185:59–66. 2014.PubMed/NCBI View Article : Google Scholar

9 

WenBo W, Fei Z, YiHeng D, Wei W, TingMang Y, WenHao Z, QianRu L and HaiTao L: Human umbilical cord mesenchymal stem cells overexpressing nerve growth factor ameliorate diabetic cystopathy in rats. Neurochem Res. 42:3537–3547. 2017.PubMed/NCBI View Article : Google Scholar

10 

Liu YD, Zhang SC, Xue J, Wei ZQ, Shen BX and Ding LC: Caffeine improves bladder function in streptozotocin-induced diabetic rats. Neurourol Urodyn. 38:81–86. 2018.PubMed/NCBI View Article : Google Scholar

11 

Kashyap M, Pore S, Yoshimura N and Tyagi P: Constitutive expression Of NGF, P75(NTR) affected by bladder distension and NGF antisense treatment. Life Sci. 148:93–98. 2016.PubMed/NCBI View Article : Google Scholar

12 

Wada N, Shimizu T, Shimizu N, de Groat WC, Kanai AJ, Tyagi P, Kakizaki H and Yoshimura N: The effect of neutralization of nerve growth factor (NGF) on bladder and urethral dysfunction in mice with spinal cord injury. Neurourol Urodyn. 37:1889–1896. 2018.PubMed/NCBI View Article : Google Scholar

13 

Tonyali S, Ates D, Akbiyik F, Kankaya D, Baydar D and Ergen A: Urine nerve growth factor (NGF) level, bladder nerve staining and symptom/problem scores in patients with interstitial cystitis. Adv Clin Exp Med. 27:159–163. 2018.PubMed/NCBI View Article : Google Scholar

14 

Kashyap MP, Pore SK, de Groat WC, Chermansky CJ, Yoshimura N and Tyagi P: BDNF overexpression in the bladder induces neuronal changes to mediate bladder overactivity. Am J Physiol Renal Physiol. 315:F45–F56. 2018.PubMed/NCBI View Article : Google Scholar

15 

Kashyap MP, Roberts C, Waseem M and Tyagi P: Drug targets in neurotrophin signaling in the central and peripheral nervous system. Mol Neurobiol. 55:6939–6955. 2018.PubMed/NCBI View Article : Google Scholar

16 

Parameswaran N, Disa J, Spielman WS, Brooks DP, Nambi P and Aiyar N: Activation of multiple mitogen-activated protein kinases by recombinant calcitonin gene-related peptide receptor. Eur J Pharmacol. 389:125–130. 2000.PubMed/NCBI View Article : Google Scholar

17 

Nirmal J, Tyagi P, Chuang YC, Lee WC, Yoshimura N, Huang CC, Rajaganapathy B and Chancellor MB: Functional and molecular characterization of hyposensitive underactive bladder tissue and urine in streptozotocin-induced diabetic rat. PLoS One. 9(e102644)2014.PubMed/NCBI View Article : Google Scholar

18 

Daneshgari F, Liu G, Birder L, Hanna-Mitchell AT and Chacko S: Diabetic bladder dysfunction: Current translational knowledge. J Urol. 182 (Suppl 6):S18–S26. 2009.PubMed/NCBI View Article : Google Scholar

19 

Li R, Wu Y, Zou S, Wang X, Li Y, Xu K, Gong F, Liu Y, Wang J, Liao Y, et al: NGF attenuates high glucose-induced ER stress, preventing schwann cell apoptosis by activating the PI3K/Akt/GSK3β and ERK1/2 pathways. Neurochem Res. 42:3005–3018. 2017.PubMed/NCBI View Article : Google Scholar

20 

Sun Z, Hu W, Yin S, Lu X, Zuo W, Ge S and Xu Y: NGF protects against oxygen and glucose deprivation-induced oxidative stress and apoptosis by up-regulation of HO-1 through MEK/ERK pathway. Neurosci Lett. 641:8–14. 2017.PubMed/NCBI View Article : Google Scholar

21 

Cao J, Wu Y, Liu G and Li Z: Over-expression of BDNF inhibits angiotensin II-induced apoptosis of cardiomyocytes in SD rats. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 34:218–224. 2018.PubMed/NCBI(In Chinese).

22 

Qi G, Mi Y, Wang Y, Li R, Huang S, Li X and Liu X: Neuroprotective action of tea polyphenols on oxidative stress-induced apoptosis through the activation of the TrkB/CREB/BDNF pathway and Keap1/Nrf2 signaling pathway in SH-SY5Y cells and mice brain. Food Funct. 8:4421–4432. 2017.PubMed/NCBI View Article : Google Scholar

23 

Ma YX, Guo Z and Sun T: CGRP inhibits norepinephrine induced apoptosis with restoration of Bcl-2/Bax in cultured cardiomyocytes of rat. Neurosci Lett. 549:130–134. 2013.PubMed/NCBI View Article : Google Scholar

24 

Yang JH, Zhang YQ and Guo Z: Endogenous CGRP protects retinal cells against stress induced apoptosis in rats. Neurosci Lett. 501:83–85. 2011.PubMed/NCBI View Article : Google Scholar

25 

Renault TT, Dejean LM and Manon S: A brewing understanding of the regulation of Bax function by Bcl-xL and Bcl-2. Mech Ageing Dev. 161:201–210. 2017.PubMed/NCBI View Article : Google Scholar

26 

Vogel MW: Cell death, Bcl-2, Bax, and the cerebellum. Cerebellum. 1:277–287. 2002.PubMed/NCBI View Article : Google Scholar

27 

Song L, Gao LN, Wang J, Thapa S, Li Y, Zhong XB, Zhao HW, Xiang XR, Zhang FG and Ji P: Stromal cell-derived factor-1α alleviates calcium-sensing receptor activation-mediated ischemia/reperfusion injury by inhibiting caspase-3/caspase-9-induced cell apoptosis in rat free flaps. Biomed Res Int. 2018(8945850)2018.PubMed/NCBI View Article : Google Scholar

28 

Lan T, Zhao H, Xiang B, Wang J and Liu Y: Suture compression induced midpalatal suture chondrocyte apoptosis with increased caspase-3, caspase-9, Bad, Bak, Bax and Bid expression. Biochem Biophys Res Commun. 489:179–186. 2017.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xue J, Liu Y, Zhang S, Ding L, Shen B, Shao Y and Wei Z: Caffeine improves bladder function in diabetic rats via a neuroprotective effect. Exp Ther Med 21: 501, 2021.
APA
Xue, J., Liu, Y., Zhang, S., Ding, L., Shen, B., Shao, Y., & Wei, Z. (2021). Caffeine improves bladder function in diabetic rats via a neuroprotective effect. Experimental and Therapeutic Medicine, 21, 501. https://doi.org/10.3892/etm.2021.9932
MLA
Xue, J., Liu, Y., Zhang, S., Ding, L., Shen, B., Shao, Y., Wei, Z."Caffeine improves bladder function in diabetic rats via a neuroprotective effect". Experimental and Therapeutic Medicine 21.5 (2021): 501.
Chicago
Xue, J., Liu, Y., Zhang, S., Ding, L., Shen, B., Shao, Y., Wei, Z."Caffeine improves bladder function in diabetic rats via a neuroprotective effect". Experimental and Therapeutic Medicine 21, no. 5 (2021): 501. https://doi.org/10.3892/etm.2021.9932
Copy and paste a formatted citation
x
Spandidos Publications style
Xue J, Liu Y, Zhang S, Ding L, Shen B, Shao Y and Wei Z: Caffeine improves bladder function in diabetic rats via a neuroprotective effect. Exp Ther Med 21: 501, 2021.
APA
Xue, J., Liu, Y., Zhang, S., Ding, L., Shen, B., Shao, Y., & Wei, Z. (2021). Caffeine improves bladder function in diabetic rats via a neuroprotective effect. Experimental and Therapeutic Medicine, 21, 501. https://doi.org/10.3892/etm.2021.9932
MLA
Xue, J., Liu, Y., Zhang, S., Ding, L., Shen, B., Shao, Y., Wei, Z."Caffeine improves bladder function in diabetic rats via a neuroprotective effect". Experimental and Therapeutic Medicine 21.5 (2021): 501.
Chicago
Xue, J., Liu, Y., Zhang, S., Ding, L., Shen, B., Shao, Y., Wei, Z."Caffeine improves bladder function in diabetic rats via a neuroprotective effect". Experimental and Therapeutic Medicine 21, no. 5 (2021): 501. https://doi.org/10.3892/etm.2021.9932
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team