Open Access

Inhibitory effect of miR-140-5p on doxorubicin resistance of hepatocellular carcinoma

  • Authors:
    • Xiaojie Gao
    • Yan Jiang
    • Yingying Li
  • View Affiliations

  • Published online on: March 18, 2021     https://doi.org/10.3892/etm.2021.9938
  • Article Number: 507
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To investigate the role of microRNA (miR)-140-5p in doxorubicin (DOX) sensitivity in hepatocellular carcinoma, miR-140-5p and peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) expression was first evaluated in hepatocellular carcinoma tissues using starBase. Next, in vitro experiments were performed. Cell line expression of miR-140-5p and PIN1 expression was detected by reverse transcription polymerase chain reaction. Cell viability and proliferation were determined by the Cell Counting Kit-8 and EdU assays. The relationship between miR-140-5p and PIN1 was evaluated by TargetScan and a luciferase reporter system. Western blotting was used to detect the expression of PIN1. It was observed that miR-140-5p was downregulated in hepatocellular carcinoma tissues and cell lines compared with normal samples in HCC or normal liver cells. Gain-of-function experiments revealed that miR-140-5p mimics were able to enhance DOX sensitivity of hepatocellular carcinoma cells. Further studies revealed that PIN1 was a target gene of miR‑140-5p. Suppression of PIN1 led to higher DOX sensitivity in hepatocellular carcinoma cells. Finally, when comparing a PIN1-siRNA alone group and a PIN1-siRNA plus miR-140-5p inhibitor group, there was no significant difference in cell viability. Furthermore, miR-140-5p mimics did not reduce the sensitivity of PIN1mut plasmid to DOX in HUH7 and SNU449 cells. The present study demonstrated that miR-140-5p could enhance DOX sensitivity in hepatocellular carcinoma cells by targeting PIN1.
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May-2021
Volume 21 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Gao X, Jiang Y and Li Y: Inhibitory effect of miR-140-5p on doxorubicin resistance of hepatocellular carcinoma. Exp Ther Med 21: 507, 2021.
APA
Gao, X., Jiang, Y., & Li, Y. (2021). Inhibitory effect of miR-140-5p on doxorubicin resistance of hepatocellular carcinoma. Experimental and Therapeutic Medicine, 21, 507. https://doi.org/10.3892/etm.2021.9938
MLA
Gao, X., Jiang, Y., Li, Y."Inhibitory effect of miR-140-5p on doxorubicin resistance of hepatocellular carcinoma". Experimental and Therapeutic Medicine 21.5 (2021): 507.
Chicago
Gao, X., Jiang, Y., Li, Y."Inhibitory effect of miR-140-5p on doxorubicin resistance of hepatocellular carcinoma". Experimental and Therapeutic Medicine 21, no. 5 (2021): 507. https://doi.org/10.3892/etm.2021.9938