Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
June-2021 Volume 21 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2021 Volume 21 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Ultrasound‑targeted microbubble destruction enhances the anti‑tumor action of miR‑4284 inhibitor in non‑small cell lung cancer cells

  • Authors:
    • Peng Tian
    • Yanzhen Wang
    • Wenyan Du
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasonics, Zibo Central Hospital, Zibo, Shandong 255036, P.R. China, Department of Science and Education, Zibo Central Hospital, Zibo, Shandong 255036, P.R. China
    Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 551
    |
    Published online on: March 25, 2021
       https://doi.org/10.3892/etm.2021.9983
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

MicroRNAs (miRNAs/miRs) are known to be involved in various human cancer types. Ultrasound‑targeted microbubble destruction (UTMD) may improve the transfection efficiency of exogenous genes into target tissues and organs, thereby improving cancer treatment. In the present study, the role of miR‑4284 in non‑small cell lung cancer (NSCLC) was investigated and the effect of UTMD‑mediated inhibition of miR‑4284 on tumor progression was further analyzed. The expression of miR‑4284 in NSCLC cells and tissues was detected by reverse transcription‑quantitative PCR. UTMD‑mediated inhibition of miR‑4284 was achieved by co‑transfection of microvesicles and miR‑4284 inhibitors into NSCLC cells. A Cell Counting Kit‑8 assay was used to determine NSCLC cell proliferation, and the migration and invasion of NSCLC cells were examined by Transwell assays. Compared with that in the control group, the expression of miR‑4284 was increased in NSCLC tissues and cells. Knockdown of miR‑4284 in NSCLC cells inhibited cell proliferation, migration and invasion. UTMD improved the transfection efficiency of miR‑4284 inhibitors in NSCLC cells, resulting in more significant inhibition of tumor cell proliferation, migration and invasion. In conclusion, the results indicated that the expression of miR‑4284 was increased in clinical samples and cell lines of NSCLC and that knockdown of miR‑4284 inhibited the proliferation, migration and invasion of tumor cells. UTMD‑mediated miR‑4284 inhibition further promoted this effect, indicating that this technique may represent a novel strategy for the treatment of NSCLC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Smith RA, Andrews KS, Brooks D, Fedewa SA, Manassaram-Baptiste D, Saslow D, Brawley OW and Wender RC: Cancer screening in the United States, 2018: A review of current American Cancer Society guidelines and current issues in cancer screening. CA Cancer J Clin. 68:297–316. 2018.PubMed/NCBI View Article : Google Scholar

2 

Miller KD, Siegel RL, Lin CC, Mariotto AB, Kramer JL, Rowland JH, Stein KD, Alteri R and Jemal A: Cancer treatment and survivorship statistics, 2016. CA Cancer J Clin. 66:271–289. 2016.PubMed/NCBI View Article : Google Scholar

3 

Travis WD, Brambilla E, Nicholson AG, Yatabe Y, Austin JHM, Beasley MB, Chirieac LR, Dacic S, Duhig E, et al: The 2015 World Health Organization Classification of Lung Tumors: Impact of Genetic, Clinical and Radiologic Advances Since the 2004 Classification. J Thorac Oncol. 10:1243–1260. 2015.PubMed/NCBI View Article : Google Scholar

4 

Cai J, Fang L, Huang Y, Li R, Xu X, Hu Z, Zhang L, Yang Y, Zhu X, Zhang H, et al: Simultaneous overactivation of Wnt/β-catenin and TGFβ signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC. Nat Commun. 8(15870)2017.PubMed/NCBI View Article : Google Scholar

5 

Chen H and Hwang JH: Ultrasound-targeted microbubble destruction for chemotherapeutic drug delivery to solid tumors. J Ther Ultrasound. 1(10)2013.PubMed/NCBI View Article : Google Scholar

6 

Tinkov S, Bekeredjian R, Winter G and Coester C: Microbubbles as ultrasound triggered drug carriers. J Pharm Sci. 98:1935–1961. 2009.PubMed/NCBI View Article : Google Scholar

7 

Yang H, Sun Y, Wei J, Xu L, Tang Y, Yang L, Zhang X and Lu Y: The effects of ultrasound targeted microbubble destruction (UTMD) carrying IL 8 monoclonal antibody on the inflammatory responses and stability of atherosclerotic plaques. Biomed Pharmacother Oct. 118(109161)2019.PubMed/NCBI View Article : Google Scholar

8 

Lee Y, Jeon K, Lee JT, Kim S and Kim VN: MicroRNA maturation: Stepwise processing and subcellular localization. EMBO J. 21:4663–4670. 2002.PubMed/NCBI View Article : Google Scholar

9 

Barbato S, Solaini G and Fabbri M: MicroRNAs in Oncogenesis and Tumor Suppression. Int Rev Cell Mol Biol. 333:229–268. 2017.PubMed/NCBI View Article : Google Scholar

10 

Dan B, Luo J, Li K and Chen S: Prognostic value of miR-375 for survival outcomes in various cancers: A systematic review and meta-analysis. Oncol Res Treat. 41:47–50. 2018.PubMed/NCBI View Article : Google Scholar

11 

Li Y, Shen Z, Jiang H, Lai Z, Wang Z, Jiang K, Ye Y and Wang S: MicroRNA 4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1. Mol Med Rep. 17:6569–6575. 2018.PubMed/NCBI View Article : Google Scholar

12 

Yang F, Nam S, Brown CE, Zhao R, Starr R, Ma Y, Xie J, Horne DA, Malkas LH, Jove R, et al: A novel berbamine derivative inhibits cell viability and induces apoptosis in cancer stem-like cells of human glioblastoma, via up-regulation of miRNA-4284 and JNK/AP-1 signaling. PLoS One. 9(e94443)2014.PubMed/NCBI View Article : Google Scholar

13 

Sim J, Kim Y, Kim H, Shin SJ, Kim DH, Paik SS and Jang K: Identification of recurrence-associated microRNAs in stage I lung adenocarcinoma. Medicine (Baltimore). 97(e10996)2018.PubMed/NCBI View Article : Google Scholar

14 

Sun W, Zhao P, Zhou Y, Xing C, Zhao L, Li Z and Yuan L: Ultrasound targeted microbubble destruction assisted exosomal delivery of miR-21 protects the heart from chemotherapy associated cardiotoxicity. Biochem Biophys Res Commun. 532:60–67. 2020.PubMed/NCBI View Article : Google Scholar

15 

Li X, Xu M, Lv W and Yang X: Ultrasound-targeted microbubble destruction-mediated miR-767 inhibition suppresses tumor progression of non-small cell lung cancer. Exp Ther Med. 19:3391–3397. 2020.PubMed/NCBI View Article : Google Scholar

16 

Leong-Poi H, Kuliszewski MA, Lekas M, Sibbald M, Teichert-Kuliszewska K, Klibanov AL, Stewart DJ and Lindner JR: Therapeutic arteriogenesis by ultrasound-mediated VEGF165 plasmid gene delivery to chronically ischemic skeletal muscle. Circ Res. 101:295–303. 2007.PubMed/NCBI View Article : Google Scholar

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

18 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015.PubMed/NCBI View Article : Google Scholar

19 

Akhurst T: Staging of non-small-cell lung cancer. PET Clin. 13:1–10. 2018.PubMed/NCBI View Article : Google Scholar

20 

Soria JC, Massard C and Le Chevalier T: Should progression-free survival be the primary measure of efficacy for advanced NSCLC therapy? Ann Oncol. 21:2324–2332. 2010.PubMed/NCBI View Article : Google Scholar

21 

Schwartz AG and Cote ML: Epidemiology of lung cancer. Adv Exp Med Biol. 893:21–41. 2016.PubMed/NCBI View Article : Google Scholar

22 

Connell LC, Harding JJ and Abou-Alfa GK: Advanced Hepatocellular Cancer: The Current State of Future Research. Curr Treat Options Oncol. 17(43)2016.PubMed/NCBI View Article : Google Scholar

23 

Wang Z, Sha HH and Li HJ: Functions and mechanisms of miR 186 in human cancer. Biomed Pharmacother. 119(109428)2019.PubMed/NCBI View Article : Google Scholar

24 

Xue L and Yang D: miR 421 inhibited proliferation and metastasis of colorectal cancer by targeting MTA1. J BUON. 23:1633–1639. 2018.PubMed/NCBI

25 

Huang L, Zhang Y, Li Z, Zhao X, Xi Z, Chen H, Shi H, Xin T, Shen R and Wang T: miR-4319 suppresses colorectal cancer progression by targeting ABTB1. United European Gastroenterol J. 7:517–528. 2019.PubMed/NCBI View Article : Google Scholar

26 

Yang G, Xiong G, Cao Z, Zheng S, You L, Zhang T and Zhao Y: miR-497 expression, function and clinical application in cancer. Oncotarget. 7:55900–55911. 2016.PubMed/NCBI View Article : Google Scholar

27 

Ji Y, Han Z, Shao L and Zhao Y: Evaluation of in vivo antitumor effects of low-frequency ultrasound-mediated miRNA-133a microbubble delivery in breast cancer. Cancer Med. 5:2534–2543. 2016.PubMed/NCBI View Article : Google Scholar

28 

Munari E, Marchionni L, Chitre A, Hayashi M, Martignoni G, Brunelli M, Gobbo S, Argani P, Allaf M, Hoque MO, et al: Clear cell papillary renal cell carcinoma: micro-RNA expression profiling and comparison with clear cell renal cell carcinoma and papillary renal cell carcinoma. Hum Pathol. 45:1130–1138. 2014.PubMed/NCBI View Article : Google Scholar

29 

Liu W, Wang P, Xie Z, Wang S, Ma M, Li J, Li M, Cen S, Tang S, Zheng G, et al: Abnormal inhibition of osteoclastogenesis by mesenchymal stem cells through the miR-4284/CXCL5 axis in ankylosing spondylitis. Cell Death Dis. 10(188)2019.PubMed/NCBI View Article : Google Scholar

30 

Tamaddon G, Geramizadeh B, Karimi MH, Mowla SJ and Abroun S: miR-4284 and miR-4484 as putative biomarkers for diffuse large B-cell lymphoma. Iran J Med Sci. 41:334–339. 2016.PubMed/NCBI

31 

Wu J and Li RK: Ultrasound-targeted microbubble destruction in gene therapy: A new tool to cure human diseases. Genes Dis. 4:64–74. 2016.PubMed/NCBI View Article : Google Scholar

32 

Shi D, Guo L, Sun X, Shang M, Meng D, Zhou X, Liu X, Zhao Y and Li J: UTMD inhibit EMT of breast cancer through the ROS/miR-200c/ZEB1 axis. Sci Rep. 10(6657)2020.PubMed/NCBI View Article : Google Scholar

33 

Lin L, Fan Y, Gao F, Jin L, Li D, Sun W, Li F, Qin P, Shi Q, Shi X, et al: UTMD-Promoted Co-Delivery of gemcitabine and miR-21 inhibitor by dendrimer-entrapped gold nanoparticles for pancreatic cancer therapy. Theranostics. 8:1923–1939. 2018.PubMed/NCBI View Article : Google Scholar

34 

Yang C, Li B, Yu J, Yang F, Cai K and Chen Z: Ultrasound microbubbles mediated miR-let-7b delivery into CD133+ ovarian cancer stem cells. Biosci Rep. 38(38)2018.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Tian P, Wang Y and Du W: Ultrasound‑targeted microbubble destruction enhances the anti‑tumor action of miR‑4284 inhibitor in non‑small cell lung cancer cells. Exp Ther Med 21: 551, 2021.
APA
Tian, P., Wang, Y., & Du, W. (2021). Ultrasound‑targeted microbubble destruction enhances the anti‑tumor action of miR‑4284 inhibitor in non‑small cell lung cancer cells. Experimental and Therapeutic Medicine, 21, 551. https://doi.org/10.3892/etm.2021.9983
MLA
Tian, P., Wang, Y., Du, W."Ultrasound‑targeted microbubble destruction enhances the anti‑tumor action of miR‑4284 inhibitor in non‑small cell lung cancer cells". Experimental and Therapeutic Medicine 21.6 (2021): 551.
Chicago
Tian, P., Wang, Y., Du, W."Ultrasound‑targeted microbubble destruction enhances the anti‑tumor action of miR‑4284 inhibitor in non‑small cell lung cancer cells". Experimental and Therapeutic Medicine 21, no. 6 (2021): 551. https://doi.org/10.3892/etm.2021.9983
Copy and paste a formatted citation
x
Spandidos Publications style
Tian P, Wang Y and Du W: Ultrasound‑targeted microbubble destruction enhances the anti‑tumor action of miR‑4284 inhibitor in non‑small cell lung cancer cells. Exp Ther Med 21: 551, 2021.
APA
Tian, P., Wang, Y., & Du, W. (2021). Ultrasound‑targeted microbubble destruction enhances the anti‑tumor action of miR‑4284 inhibitor in non‑small cell lung cancer cells. Experimental and Therapeutic Medicine, 21, 551. https://doi.org/10.3892/etm.2021.9983
MLA
Tian, P., Wang, Y., Du, W."Ultrasound‑targeted microbubble destruction enhances the anti‑tumor action of miR‑4284 inhibitor in non‑small cell lung cancer cells". Experimental and Therapeutic Medicine 21.6 (2021): 551.
Chicago
Tian, P., Wang, Y., Du, W."Ultrasound‑targeted microbubble destruction enhances the anti‑tumor action of miR‑4284 inhibitor in non‑small cell lung cancer cells". Experimental and Therapeutic Medicine 21, no. 6 (2021): 551. https://doi.org/10.3892/etm.2021.9983
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team