Oxymatrine pretreatment protects H9c2 cardiomyocytes from hypoxia/reoxygenation injury by modulating the PI3K/Akt pathway

Zhang,Zhongbai; Qin,Xueting; Wang,Zhenghui; Li,Yanchun; Chen,Fei; Chen,Rundu; Li,Chuang; Zhang,Wencheng; Zhang,Mei

doi:10.3892/etm.2021.9988

Oxymatrine pretreatment protects H9c2 cardiomyocytes from hypoxia/reoxygenation injury by modulating the PI3K/Akt pathway

Authors:
- Zhongbai Zhang
- Xueting Qin
- Zhenghui Wang
- Yanchun Li
- Fei Chen
- Rundu Chen
- Chuang Li
- Wencheng Zhang
- Mei Zhang
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Affiliations: The Fourth Detachment, Armed Police and Coastal Police Corps, Wenchang, Hainan 571300, P.R. China, Department of Cardiology, The Third People's Hospital of Jingzhou, Jingzhou, Hubei 434000, P.R. China, Department of Human Morphology Section, Logistics University of People's Armed Police Force, Tianjin 300162, P.R. China, Department of Pharmacy, Heilongjiang Municipal Corps Hospital of Chinese People's Armed Police Force, Harbin, Heilongjiang 150076, P.R. China, Department of Health Service, The Second Mobile Corps Hospital of Chinese People's Armed Police Force, Wuxi, Jiangsu 214000, P.R. China, Department of Cardiac Thoracic Surgery, Characteristic Medical Center of People's Armed Police Force, Tianjin 300309, P.R. China, Tianjin Key Laboratory of Hepatopancreatic Fibrosis and Molecular Diagnosis and Treatment, Characteristic Medical Center of People's Armed Police Force, Tianjin 300162, P.R. China
Published online on: March 26, 2021 https://doi.org/10.3892/etm.2021.9988
Article Number: 556
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ischemia‑reperfusion (I/R) plays an important role in myocardial damage, which has been widely recognized as a key procedure in the cardiovascular disease. A hypoxia/reoxygenation (H/R) model was established using H9c2 cardiomyocytes to investigate the possible positive effect of oxymatrine (OMT), an alkaloid originating from the traditional Chinese herb Sophora flavescens Aiton, on cardiomyocytes exposed to H/R injury and the underlying molecular mechanisms. Cell viability was measured using the MTT assay, lactate dehydrogenase release measurements and hematoxylin and eosin staining. Oxidative stress was detected by measuring cellular malondialdehyde (MDA) content, as well as superoxide dismutase (SOD) and catalase (CAT) activities. Apoptosis was detected using TUNEL staining and flow cytometric analysis, and the underlying mechanism was investigated using reverse transcription‑quantitative PCR and western blot analyses. The results revealed that OMT increased the viability of H9c2 cardiomyocytes exposed to H/R. The OMT pretreatment decreased the production of MDA by reactive oxygen species and increased the activities of SOD and CAT. Furthermore, the OMT pretreatment reduced the expression of Bax and caspase‑3, while inducing Bcl‑2 expression. In addition, the protective effect of OMT was shown to be associated with the PI3K/Akt signaling pathway, and the PI3K inhibitor LY294002 attenuated the effects of OMT on the H9c2 cardiomyocytes exposed to H/R. These findings indicate that OMT could be a potential therapeutic candidate for the treatment of myocardial ischemia/reperfusion injury.

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