Open Access

Long non‑coding RNA ADAMTS9‑AS2 inhibits liver cancer cell proliferation, migration and invasion

  • Authors:
    • Hanjun Li
    • Hu Huang
    • Sha Li
    • Hongliang Mei
    • Tingjia Cao
    • Qiping Lu
  • View Affiliations

  • Published online on: March 26, 2021     https://doi.org/10.3892/etm.2021.9991
  • Article Number: 559
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNA (lncRNA) ADAM metallopeptidase with thrombospondin type 1 motif 9 antisense RNA 2 (ADAMTS9‑AS2) is involved in various types of cancer, such as ovarian cancer, lung cancer and clear cell renal cell carcinoma. However, the roles of ADAMTS9‑AS2 in liver cancer are not completely understood. The present study aimed to determine the functional role of ADAMTS9‑AS2 in human liver cancer and investigate the potential underlying molecular mechanisms. The expression levels of ADAMTS9‑AS2 and ADAMTS9 were determined following ADAMTS9‑AS2 overexpression and knockdown. The results indicated that ADAMTS9‑AS2 overexpression and knockdown increased and decreased ADAMTS9 mRNA and protein expression levels, respectively, indicating that alterations in ADAMTS9 expression corresponded with ADAMTS9‑AS2 expression. Subsequently, the effects of ADAMTS9‑AS2 on liver cancer cell proliferation, migration and invasion were analyzed by performing Cell Counting Kit‑8, wound healing and Transwell assays, respectively. The results demonstrated that ADAMTS9‑AS2 inhibited liver cancer cell proliferation, migration and invasion. Finally, the effect of ADAMTS9 on PI3K/AKT/mTOR signaling pathway‑associated proteins [AKT, phosphorylated‑AKT, phosphatidylinositol‑4, 5‑bisphosphate 3‑kinase catalytic subunit β (PIK3CB), mTOR and phosphorylated‑mTOR], several key autophagy‑related proteins [light chain 3‑I/II (LC3‑I/II), beclin 1 (BECN1) and sequestosome 1 (SQSTM1)] and apoptosis‑related proteins (Bax and Bcl‑2) was detected via western blotting. The results suggested that ADAMTS9‑AS2 downregulated the phosphorylation of AKT and mTOR, the protein expression level of PIK3CB, as well as the expression levels of autophagy protein SQSTM1 and antiapoptotic protein Bcl‑2. By contrast, ADAMTS9‑AS2 upregulated the expression levels of autophagy proteins LC3‑II and BECN1, and the proapoptotic protein Bax. Collectively, ADAMTS9‑AS2 inhibited liver cancer cell proliferation, migration and invasion via inhibiting the PI3K/AKT/mTOR signaling pathway. The present study provided a novel insight into the role of ADAMTS9‑AS2 in liver cancer.
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June-2021
Volume 21 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Li H, Huang H, Li S, Mei H, Cao T and Lu Q: Long non‑coding RNA ADAMTS9‑AS2 inhibits liver cancer cell proliferation, migration and invasion. Exp Ther Med 21: 559, 2021
APA
Li, H., Huang, H., Li, S., Mei, H., Cao, T., & Lu, Q. (2021). Long non‑coding RNA ADAMTS9‑AS2 inhibits liver cancer cell proliferation, migration and invasion. Experimental and Therapeutic Medicine, 21, 559. https://doi.org/10.3892/etm.2021.9991
MLA
Li, H., Huang, H., Li, S., Mei, H., Cao, T., Lu, Q."Long non‑coding RNA ADAMTS9‑AS2 inhibits liver cancer cell proliferation, migration and invasion". Experimental and Therapeutic Medicine 21.6 (2021): 559.
Chicago
Li, H., Huang, H., Li, S., Mei, H., Cao, T., Lu, Q."Long non‑coding RNA ADAMTS9‑AS2 inhibits liver cancer cell proliferation, migration and invasion". Experimental and Therapeutic Medicine 21, no. 6 (2021): 559. https://doi.org/10.3892/etm.2021.9991