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Alliin inhibits adipocyte differentiation by downregulating Akt expression: Implications for metabolic disease

  • Authors:
    • Ni Li
    • Kai Chen
    • Hongwei Dong
    • Jing Yang
    • Michiko Yoshizawa
    • Hideaki Kagami
    • Xianqi Li
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Zhongshan Hospital, Fudan University, Shanghai 200031, P.R. China, Department of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China, Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University, Shiojiri, Nagano 399‑0781, Japan, Department of Hard Tissue Research, Institute for Oral Science, Matsumoto Dental University, Shiojiri, Nagano 399‑0781, Japan
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 563
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    Published online on: March 26, 2021
       https://doi.org/10.3892/etm.2021.9995
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Abstract

Obesity is currently an important health problem and is associated with an increased likelihood of various diseases. The efficacies of various natural treatments have been assessed for their utility in treating obesity. Alliin (S‑allyl‑L‑cysteine sulfoxides) is considered the major component of garlic and has a wide range of natural antioxidant properties. However, the direct effects of alliin on obesity have not been well clarified. The present study investigated the effects and possible mechanisms of alliin on adipocyte differentiation. The 3T3‑L1 cells were treated with alliin (0‑40 µg/ml) during adipogenic differentiation. The effect of alliin on lipid accumulation was evaluated by Oil red O staining. Reverse transcription‑quantitative PCR was performed to investigate the expression levels of adipogenic differentiation‑related genes. The accumulation of lipid droplets was markedly inhibited following alliin treatment. The expression levels of multiple adipogenic transcription markers, such as CCAAT/enhancer‑binding protein (C/EBP) β, C/EBP α and peroxisome proliferation‑activity receptor γ, were markedly decreased following treatment with alliin during adipogenic differentiation. Expression levels of several adipocyte‑related genes were subsequently suppressed. Additionally, alliin suppressed PKB/Akt and PI3K expression. These results suggested that alliin exhibits anti‑adipogenic activity by downregulating major adipogenic differentiation‑related genes and Akt/PI3K expression. Alliin may have a potential therapeutic effect on metabolic disease.
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Copy and paste a formatted citation
Spandidos Publications style
Li N, Chen K, Dong H, Yang J, Yoshizawa M, Kagami H and Li X: Alliin inhibits adipocyte differentiation by downregulating Akt expression: Implications for metabolic disease. Exp Ther Med 21: 563, 2021.
APA
Li, N., Chen, K., Dong, H., Yang, J., Yoshizawa, M., Kagami, H., & Li, X. (2021). Alliin inhibits adipocyte differentiation by downregulating Akt expression: Implications for metabolic disease. Experimental and Therapeutic Medicine, 21, 563. https://doi.org/10.3892/etm.2021.9995
MLA
Li, N., Chen, K., Dong, H., Yang, J., Yoshizawa, M., Kagami, H., Li, X."Alliin inhibits adipocyte differentiation by downregulating Akt expression: Implications for metabolic disease". Experimental and Therapeutic Medicine 21.6 (2021): 563.
Chicago
Li, N., Chen, K., Dong, H., Yang, J., Yoshizawa, M., Kagami, H., Li, X."Alliin inhibits adipocyte differentiation by downregulating Akt expression: Implications for metabolic disease". Experimental and Therapeutic Medicine 21, no. 6 (2021): 563. https://doi.org/10.3892/etm.2021.9995
Copy and paste a formatted citation
x
Spandidos Publications style
Li N, Chen K, Dong H, Yang J, Yoshizawa M, Kagami H and Li X: Alliin inhibits adipocyte differentiation by downregulating Akt expression: Implications for metabolic disease. Exp Ther Med 21: 563, 2021.
APA
Li, N., Chen, K., Dong, H., Yang, J., Yoshizawa, M., Kagami, H., & Li, X. (2021). Alliin inhibits adipocyte differentiation by downregulating Akt expression: Implications for metabolic disease. Experimental and Therapeutic Medicine, 21, 563. https://doi.org/10.3892/etm.2021.9995
MLA
Li, N., Chen, K., Dong, H., Yang, J., Yoshizawa, M., Kagami, H., Li, X."Alliin inhibits adipocyte differentiation by downregulating Akt expression: Implications for metabolic disease". Experimental and Therapeutic Medicine 21.6 (2021): 563.
Chicago
Li, N., Chen, K., Dong, H., Yang, J., Yoshizawa, M., Kagami, H., Li, X."Alliin inhibits adipocyte differentiation by downregulating Akt expression: Implications for metabolic disease". Experimental and Therapeutic Medicine 21, no. 6 (2021): 563. https://doi.org/10.3892/etm.2021.9995
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