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MicroRNA‑21‑5p acts via the PTEN/Akt/FOXO3a signaling pathway to prevent cardiomyocyte injury caused by high glucose/high fat conditions

  • Authors:
    • Ying Han
    • Xiaoqin Cai
    • Min Pan
    • Jin Gong
    • Wenqin Cai
    • Dan Lu
    • Changsheng Xu
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Department of General Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Fujian Institute of Hypertension, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 230
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    Published online on: January 18, 2022
       https://doi.org/10.3892/etm.2022.11154
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Abstract

MicroRNAs (miRNAs or miRs) play important roles in cardiovascular disease. miR‑21‑5p is known to be involved in the regulation of cardiomyocyte injury under high glucose and high fat (HG‑HF) conditions, but its mechanism of action remains unclear. In the present study, a cardiomyocyte cell line, H9c2, was treated with 33 mM glucose and 250 µM sodium palmitate for 24, 48, and 72 h to produce HG‑HF injury. After treatment, miR‑21‑5p expression was detected by reverse transcription‑quantitative PCR. A miR‑21‑5p mimic was then constructed and transfected into the cells and the potential molecular mechanism was investigated using Cell Counting Kit‑8, TUNEL, flow cytometry and western blot assays. Expression of miR‑21‑5p was significantly downregulated by HG‑HF treatment of H9c2 cells for 24, 48, and 72 h. In subsequent experiments, cells were treated for an intermediate period (48 h). Compared with the control group, HG‑HF treatment significantly inhibited H9c2 proliferation and promoted apoptosis, while these effects were significantly reduced in the miR‑21‑5p mimic. Compared with the control group, HG‑HF treatment significantly increased reactive oxygen species, while miR‑21‑5p mimic significantly reduced this effect. Compared with the control group, HG‑HF treatment significantly increased the expression of the pro‑apoptotic proteins Bax and phosphorylated (p)‑Akt and decreased the expression of the anti‑apoptotic proteins Bcl‑2, p‑PTEN, and p‑FOXO3a, while overexpression of miR‑21‑5p significantly reduced these effects. The results revealed that miR‑21‑5p inhibited apoptosis and oxidative stress in H9c2 cells induced by HG‑HF, likely through the PTEN/Akt/FOXO3a signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Han Y, Cai X, Pan M, Gong J, Cai W, Lu D and Xu C: MicroRNA‑21‑5p acts via the PTEN/Akt/FOXO3a signaling pathway to prevent cardiomyocyte injury caused by high glucose/high fat conditions. Exp Ther Med 23: 230, 2022.
APA
Han, Y., Cai, X., Pan, M., Gong, J., Cai, W., Lu, D., & Xu, C. (2022). MicroRNA‑21‑5p acts via the PTEN/Akt/FOXO3a signaling pathway to prevent cardiomyocyte injury caused by high glucose/high fat conditions. Experimental and Therapeutic Medicine, 23, 230. https://doi.org/10.3892/etm.2022.11154
MLA
Han, Y., Cai, X., Pan, M., Gong, J., Cai, W., Lu, D., Xu, C."MicroRNA‑21‑5p acts via the PTEN/Akt/FOXO3a signaling pathway to prevent cardiomyocyte injury caused by high glucose/high fat conditions". Experimental and Therapeutic Medicine 23.3 (2022): 230.
Chicago
Han, Y., Cai, X., Pan, M., Gong, J., Cai, W., Lu, D., Xu, C."MicroRNA‑21‑5p acts via the PTEN/Akt/FOXO3a signaling pathway to prevent cardiomyocyte injury caused by high glucose/high fat conditions". Experimental and Therapeutic Medicine 23, no. 3 (2022): 230. https://doi.org/10.3892/etm.2022.11154
Copy and paste a formatted citation
x
Spandidos Publications style
Han Y, Cai X, Pan M, Gong J, Cai W, Lu D and Xu C: MicroRNA‑21‑5p acts via the PTEN/Akt/FOXO3a signaling pathway to prevent cardiomyocyte injury caused by high glucose/high fat conditions. Exp Ther Med 23: 230, 2022.
APA
Han, Y., Cai, X., Pan, M., Gong, J., Cai, W., Lu, D., & Xu, C. (2022). MicroRNA‑21‑5p acts via the PTEN/Akt/FOXO3a signaling pathway to prevent cardiomyocyte injury caused by high glucose/high fat conditions. Experimental and Therapeutic Medicine, 23, 230. https://doi.org/10.3892/etm.2022.11154
MLA
Han, Y., Cai, X., Pan, M., Gong, J., Cai, W., Lu, D., Xu, C."MicroRNA‑21‑5p acts via the PTEN/Akt/FOXO3a signaling pathway to prevent cardiomyocyte injury caused by high glucose/high fat conditions". Experimental and Therapeutic Medicine 23.3 (2022): 230.
Chicago
Han, Y., Cai, X., Pan, M., Gong, J., Cai, W., Lu, D., Xu, C."MicroRNA‑21‑5p acts via the PTEN/Akt/FOXO3a signaling pathway to prevent cardiomyocyte injury caused by high glucose/high fat conditions". Experimental and Therapeutic Medicine 23, no. 3 (2022): 230. https://doi.org/10.3892/etm.2022.11154
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