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miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6

  • Authors:
    • Liangpan Shi
    • Yibin Su
    • Zhihua Zheng
    • Jinyu Qi
    • Weidong Wang
    • Cunchuan Wang
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    Affiliations: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China, Department of Gastrointestinal Surgery, The First Hospital of Quanzhou Affiliated of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 231
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    Published online on: January 20, 2022
       https://doi.org/10.3892/etm.2022.11155
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Abstract

Increasing evidence highlights the multiple roles of microRNAs (miRs) in the tumorigenesis of colorectal cancer (CRC); however, the molecular mechanism, particularly the target of miR‑146b‑5p in CRC has not been fully elucidated. The present study aimed to elucidate the influence of miR‑146b‑5p via regulating tumor necrosis factor receptor‑associated factor 6 (TRAF6) in CRC. The expression levels of miR‑146b‑5p and TRAF6 in CRC tissue and cells were determined by reverse transcription quantitative PCR and western blotting. Binding between miR‑146b‑5p and TRAF6 was examined using a dual luciferase reporter gene assay. The impact of miR‑146b‑5p and TRAF6 on proliferation and migration of CRC cells was determined using Cell Counting Kit‑8 and Transwell assays, respectively. An animal model of CRC was established to determine the carcinogenic effect of the miR‑146b‑5p‑TRAF6 axis. The results demonstrated that miR‑146b‑5p was highly expressed in CRC tissue samples compared with in normal adjacent tissue samples and in CRC cells compared with in the normal NCM460 cell line, whereas TRAF6 was expressed at low levels. Overexpression of miR‑146b‑5p decreased TRAF6 expression in CRC HT29 and SW620 cells. miR‑146b‑5p targeted and inhibited TRAF6 expression in CRC cells. Furthermore, transfection with a miR‑146b‑5p mimic promoted the proliferation, migration and invasion of CRC cells and tumor growth; however, these effects were abolished by TRAF6 overexpression. Transfection with a miR‑146b‑5p inhibitor suppressed the proliferation of CRC cells. Taken together, the results from the present study demonstrated that miR‑146b‑5p could enhance the initiation and tumorigenesis of CRC by targeting TRAF6. These results will help elucidate the mechanisms underlying CRC development and will facilitate the development of targeted therapy for CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Shi L, Su Y, Zheng Z, Qi J, Wang W and Wang C: miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6. Exp Ther Med 23: 231, 2022.
APA
Shi, L., Su, Y., Zheng, Z., Qi, J., Wang, W., & Wang, C. (2022). miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6. Experimental and Therapeutic Medicine, 23, 231. https://doi.org/10.3892/etm.2022.11155
MLA
Shi, L., Su, Y., Zheng, Z., Qi, J., Wang, W., Wang, C."miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6". Experimental and Therapeutic Medicine 23.3 (2022): 231.
Chicago
Shi, L., Su, Y., Zheng, Z., Qi, J., Wang, W., Wang, C."miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6". Experimental and Therapeutic Medicine 23, no. 3 (2022): 231. https://doi.org/10.3892/etm.2022.11155
Copy and paste a formatted citation
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Spandidos Publications style
Shi L, Su Y, Zheng Z, Qi J, Wang W and Wang C: miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6. Exp Ther Med 23: 231, 2022.
APA
Shi, L., Su, Y., Zheng, Z., Qi, J., Wang, W., & Wang, C. (2022). miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6. Experimental and Therapeutic Medicine, 23, 231. https://doi.org/10.3892/etm.2022.11155
MLA
Shi, L., Su, Y., Zheng, Z., Qi, J., Wang, W., Wang, C."miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6". Experimental and Therapeutic Medicine 23.3 (2022): 231.
Chicago
Shi, L., Su, Y., Zheng, Z., Qi, J., Wang, W., Wang, C."miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6". Experimental and Therapeutic Medicine 23, no. 3 (2022): 231. https://doi.org/10.3892/etm.2022.11155
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