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Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulation

  • Authors:
    • Min Lei
    • Guibao Ke
    • Yan Wang
    • Dan Luo
    • Yao Hu
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Affiliated Hospital and Clinical Medical College of Chengdu University, Jinniu, Chengdu, Sichuan 610081, P.R. China
    Copyright: © Lei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 236
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    Published online on: January 24, 2022
       https://doi.org/10.3892/etm.2022.11161
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Abstract

Podocyte apoptosis and mitochondrial dysfunction serve a major role in diabetic nephropathy progression. The present study revealed a molecular mechanism regulating podocyte apoptosis and mitochondrial dysfunction. In vitro models were established using conditionally immortalized mouse podocyte clonal cells treated with high glucose (HG). Reverse quantitative‑transcription PCR were used to detect gene expression, western blotting and immunofluorescence were used to detect protein expression, Cell Counting Kit‑8 was used to detect cell viability and flow cytometry was used to detect cell apoptosis. HG treatment in the mouse podocyte clonal cells downregulated taurine‑upregulated gene 1 (TUG1) expression and decreased viability in a dose‑dependent manner. In addition, TUG1 knockdown (KD) increased HG‑induced apoptosis, while TUG1 overexpression (OE) reduced HG‑induced apoptosis in podocytes. HG‑induced mitochondrial dysfunction was identified in podocytes, with increased reactive oxygen species levels, decreased complex I/III activity and decreased basal/maximal oxygen consumption rate. TUG1 KD worsened HG‑induced mitochondrial dysfunction, and TUG1 OE reversed these effects. At the molecular level, TUG1 was revealed to promote sirtuin 1 (SIRT1) expression by sponging microRNA (miR)‑9, and SIRT1 OE reversed the HG‑induced apoptosis and mitochondrial dysfunction increased by TUG1 KD. The present data indicated that downregulation of TUG1 induced by HG was associated with HG‑induced apoptosis and mitochondrial dysfunction in podocytes, and that TUG1 protected HG‑induced podocytes by promoting SIRT1 expression via miR‑9 inhibition.
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1 

Alicic RZ, Rooney MT and Tuttle KR: Diabetic kidney disease: Challenges, progress, and possibilities. Clin J Am Soc Nephrol. 12:2032–2045. 2017.PubMed/NCBI View Article : Google Scholar

2 

Altintas MM and Reiser J: Podocytes: Way to Go. Am J Pathol. 189:226–228. 2019.PubMed/NCBI View Article : Google Scholar

3 

Lin JS and Susztak KJ: Podocytes: the weakest link in diabetic kidney disease? Curr Diab Rep. 16(45)2016.PubMed/NCBI View Article : Google Scholar

4 

Forbes JM and Thorburn DR: Mitochondrial dysfunction in diabetic kidney disease. Nat Rev Nephrol. 14:291–312. 2018.PubMed/NCBI View Article : Google Scholar

5 

Wei PZ and Szeto CC: Mitochondrial dysfunction in diabetic kidney disease. Clin Chim Acta. 496:108–116. 2019.PubMed/NCBI View Article : Google Scholar

6 

Piwkowska A, Rogacka D, Jankowski M, Dominiczak MH, Stepiński JK and Angielski S: Metformin induces suppression of NAD (P) H oxidase activity in podocytes. Biochem Biophys Res Commun. 393:268–273. 2010.PubMed/NCBI View Article : Google Scholar

7 

Lee SH, Moon SJ, Paeng J, Kang HY, Nam BY, Kim S, Kim CH, Lee MJ, Oh HJ, Park JT, et al: Podocyte hypertrophy precedes apoptosis under experimental diabetic conditions. Apoptosis. 20:1056–1071. 2015.PubMed/NCBI View Article : Google Scholar

8 

Long J, Badal SS, Ye Z, Wang Y, Ayanga BA, Galvan DL, Green NH, Chang BH, Overbeek PA and Danesh FR: Long noncoding RNA Tug1 regulates mitochondrial bioenergetics in diabetic nephropathy. J Clin Invest. 126:4205–4218. 2016.PubMed/NCBI View Article : Google Scholar

9 

Yue P, Jing L, Zhao X, Zhu H and Teng J: Down-regulation of taurine-up-regulated gene 1 attenuates inflammation by sponging miR-9-5p via targeting NF-κB1/p50 in multiple sclerosis. Life Sci. 233(116731)2019.PubMed/NCBI View Article : Google Scholar

10 

Hong Q, Zhang L, Das B, Li Z, Liu B, Cai G, Chen X, Chuang PY, He JC and Lee K: Increased podocyte Sirtuin-1 function attenuates diabetic kidney injury. Kidney Int. 93:1330–1343. 2018.PubMed/NCBI View Article : Google Scholar

11 

Zhang Y, Chang B, Zhang J and Wu X: LncRNA SOX2OT alleviates the high glucose-induced podocytes injury through autophagy induction by the miR-9/SIRT1 axis. Exp Mol Pathol. 110(104283)2019.PubMed/NCBI View Article : Google Scholar

12 

Cai X, Bao L, Ren J, Li Y and Zhang Z: Grape seed procyanidin B2 protects podocytes from high glucose-induced mitochondrial dysfunction and apoptosis via the AMPK-SIRT1-PGC-1α axis in vitro. Food Funct. 7:805–815. 2016.PubMed/NCBI View Article : Google Scholar

13 

Lei X, Zhang L, Li Z and Ren J: Astragaloside IV/lncRNA-TUG1/TRAF5 signaling pathway participates in podocyte apoptosis of diabetic nephropathy rats. Drug Des Devel Ther. 12:2785–2793. 2018.PubMed/NCBI View Article : Google Scholar

14 

Chen J, Gui D, Chen Y, Mou L, Liu Y and Huang J: Astragaloside IV improves high glucose-induced podocyte adhesion dysfunction via alpha3beta1 integrin upregulation and integrin-linked kinase inhibition. Biochem Pharmacol. 76:796–804. 2008.PubMed/NCBI View Article : Google Scholar

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-ΔΔC(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

16 

Chen ZZ, Huang L, Wu YH, Zhai WJ and Gao YF: LncSox4 promotes the self-renewal of liver tumour-initiating cells through Stat3-mediated Sox4 expression. Nat Commun. 7(12598)2016.PubMed/NCBI View Article : Google Scholar

17 

Jie Tao J, Zhang J, Ling Y, McCall CE and Liu TF: Mitochondrial Sirtuin 4 resolves immune tolerance in monocytes by rebalancing glycolysis and glucose oxidation homeostasis. Front Immunol. 9(419)2018.PubMed/NCBI View Article : Google Scholar

18 

Long J, Badal SS, Ye Z, Wang Y, Ayanga BA, Galvan DL, Green NH, Chang BH, Overbeek PA and Danesh FR: Long noncoding RNA Tug1 regulates mitochondrial bioenergetics in diabetic nephropathy. J Clin Invest. 126:4205–4218. 2016.PubMed/NCBI View Article : Google Scholar

19 

Maestroni S and Zerbini G: Glomerular endothelial cells versus podocytes as the cellular target in diabetic nephropathy. Acta Diabetol. 55:1105–1111. 2018.PubMed/NCBI View Article : Google Scholar

20 

Shang X, Ji X, Dang J, Wang L, Sun C, Liu K, Sik A and Jin M: α-asarone induces cardiac defects and QT prolongation through mitochondrial apoptosis pathway in zebrafish. Toxicol Lett. 324:1–11. 2020.PubMed/NCBI View Article : Google Scholar

21 

Yang B, Chen Y and Shi J: Reactive oxygen species (ROS)-based nanomedicine. Chem Rev. 119:4881–4985. 2019.PubMed/NCBI View Article : Google Scholar

22 

Turrens JF: Mitochondrial formation of reactive oxygen species. J Physiol. 552:335–344. 2003.PubMed/NCBI View Article : Google Scholar

23 

Wang S, Cheng M, Zheng X, Zheng L, Liu H, Lu J, Liu Y and Chen W: Interactions between lncRNA TUG1 and miR-9 modulate the resistance of breast cancer cells to doxorubicin by regulating eIF5A2. Onco Targets Ther. 13:13159–13170. 2020.PubMed/NCBI View Article : Google Scholar

24 

Zhang Y, Chang B, Zhang J and Wu X: LncRNA SOX2OT alleviates the high glucose-induced podocytes injury through autophagy induction by the miR-9/SIRT1 axis. Exp Mol Pathol. 110(104283)2019.PubMed/NCBI View Article : Google Scholar

25 

Lagouge M, Argmann C, Gerhart-Hines Z, Meziane H, Lerin C, Daussin F, Messadeq N, Milne J, Lambert P, Elliott P, et al: Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1α. Cell. 127:1109–1122. 2006.PubMed/NCBI View Article : Google Scholar

26 

Yousuf S, Atif F, Ahmad M, Hoda N, Ishrat T, Khan B and Islam F: Resveratrol exerts its neuroprotective effect by modulating mitochondrial dysfunctions and associated cell death during cerebral ischemia. Brain Res. 1250:242–253. 2009.PubMed/NCBI View Article : Google Scholar

27 

Zhao Y, Li H, Fang S, Kang Y, Wu W, Hao Y, Li Z, Bu D, Sun N, Zhang MQ, et al: NONCODE 2016: an informative and valuable data source of long non-coding RNAs. Nucleic Acids Res. 44:D203–D208. 2016.PubMed/NCBI View Article : Google Scholar

28 

Ulitsky I: Evolution to the rescue: using comparative genomics to understand long non-coding RNAs. Nat Rev Genet. 17:601–614. 2016.PubMed/NCBI View Article : Google Scholar

29 

Militti C, Maenner S, Becker PB and Gebauer F: UNR facilitates the interaction of MLE with the lncRNA roX2 during Drosophila dosage compensation. Nat Commun. 5(4762)2014.PubMed/NCBI View Article : Google Scholar

30 

Rinn JL and Chang HY: Long noncoding RNAs: molecular modalities to organismal functions. Annu Rev Biochem. 89:283–308. 2020.PubMed/NCBI View Article : Google Scholar

31 

Li SY and Susztak K: The long noncoding RNA Tug1 connects metabolic changes with kidney disease in podocytes. J Clin Invest. 126:4072–4075. 2016.PubMed/NCBI View Article : Google Scholar

32 

Li Y, Huang D, Zheng L, Cao H, Gao Y, Yanga Y and Fan Z: Long non-coding RNA TUG1 alleviates high glucose induced podocyte inflammation, fibrosis and apoptosis in diabetic nephropathy via targeting the miR-27a-3p/E2F3 axis. RSC Adv. 9:37620–37629. 2019.

33 

Lei X, Zhang L, Li Z and Ren J: Astragaloside IV/lncRNA-TUG1/TRAF5 signaling pathway participates in podocyte apoptosis of diabetic nephropathy rats. Drug Des Devel Ther. 12:2785–2793. 2018.PubMed/NCBI View Article : Google Scholar

34 

Roger AJ, Muñoz-Gómez SA and Kamikawa R: The origin and diversification of mitochondria. Curr Biol. 27:R1177–R1192. 2017.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Lei M, Ke G, Wang Y, Luo D and Hu Y: Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulation. Exp Ther Med 23: 236, 2022.
APA
Lei, M., Ke, G., Wang, Y., Luo, D., & Hu, Y. (2022). Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulation. Experimental and Therapeutic Medicine, 23, 236. https://doi.org/10.3892/etm.2022.11161
MLA
Lei, M., Ke, G., Wang, Y., Luo, D., Hu, Y."Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulation". Experimental and Therapeutic Medicine 23.3 (2022): 236.
Chicago
Lei, M., Ke, G., Wang, Y., Luo, D., Hu, Y."Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulation". Experimental and Therapeutic Medicine 23, no. 3 (2022): 236. https://doi.org/10.3892/etm.2022.11161
Copy and paste a formatted citation
x
Spandidos Publications style
Lei M, Ke G, Wang Y, Luo D and Hu Y: Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulation. Exp Ther Med 23: 236, 2022.
APA
Lei, M., Ke, G., Wang, Y., Luo, D., & Hu, Y. (2022). Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulation. Experimental and Therapeutic Medicine, 23, 236. https://doi.org/10.3892/etm.2022.11161
MLA
Lei, M., Ke, G., Wang, Y., Luo, D., Hu, Y."Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulation". Experimental and Therapeutic Medicine 23.3 (2022): 236.
Chicago
Lei, M., Ke, G., Wang, Y., Luo, D., Hu, Y."Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulation". Experimental and Therapeutic Medicine 23, no. 3 (2022): 236. https://doi.org/10.3892/etm.2022.11161
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