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Article Open Access

LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway

  • Authors:
    • Yihui Xu
    • Hubo Shi
    • Min Wang
    • Ping Huang
    • Mingjie Xu
    • Shuyi Han
    • Huanjie Li
    • Yunshan Wang
  • View Affiliations / Copyright

    Affiliations: Medical Research and Laboratory Diagnostic Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250013, P.R. China, Department of Thoracic Surgery, Shangdong Public Health Clinical Center, Jinan, Shandong 250102, P.R. China, School of Medicine, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 247
    |
    Published online on: January 28, 2022
       https://doi.org/10.3892/etm.2022.11172
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Abstract

Lung cancer causes thousands of deaths worldwide every year, and present therapeutics show little benefit for advanced‑stage patients. Researchers do not know why and how lung cancer begins. Lactamase β (LACTB) is a tumor‑suppressor in some cancers. However, its role in lung cancer is unknown. By analyzing the TCGA database and Kaplan‑Meier Plotter database, LACTB was found to be downregulated in lung cancer tissues but the methylation level was increased. Patients with high LACTB expression exhibited improved survival. Then, in vitro assays demonstrated that LACTB overexpression inhibited cell migration and invasion, and induced apoptosis in H1299 and H1975 cells. Knockdown of LACTB caused the reverse effects. Moreover, a much higher apoptotic rate and more potent inhibitory effects on H1299 and H1975 cells were obtained when LACTB was combined with docetaxel. In addition, members of the epithelial‑mesenchymal transition (EMT) signaling pathway were assessed using western blot analysis. The expression of E‑cadherin was decreased while levels of N‑cadherin and vimentin were increased after knockdown of LACTB in lung cancer cells. By contrast, overexpression of LACTB increased the level of E‑cadherin but decreased N‑cadherin and vimentin. Therefore, LACTB is a tumor suppressor in lung cancer that inhibits cell migration and invasion and induces cell apoptosis. Meanwhile, LACTB was found to strengthen the anticancer role of docetaxel and to suppress the EMT pathway in lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Shi H, Wang M, Huang P, Xu M, Han S, Li H and Wang Y: LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway. Exp Ther Med 23: 247, 2022.
APA
Xu, Y., Shi, H., Wang, M., Huang, P., Xu, M., Han, S. ... Wang, Y. (2022). LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway. Experimental and Therapeutic Medicine, 23, 247. https://doi.org/10.3892/etm.2022.11172
MLA
Xu, Y., Shi, H., Wang, M., Huang, P., Xu, M., Han, S., Li, H., Wang, Y."LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway". Experimental and Therapeutic Medicine 23.3 (2022): 247.
Chicago
Xu, Y., Shi, H., Wang, M., Huang, P., Xu, M., Han, S., Li, H., Wang, Y."LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway". Experimental and Therapeutic Medicine 23, no. 3 (2022): 247. https://doi.org/10.3892/etm.2022.11172
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Shi H, Wang M, Huang P, Xu M, Han S, Li H and Wang Y: LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway. Exp Ther Med 23: 247, 2022.
APA
Xu, Y., Shi, H., Wang, M., Huang, P., Xu, M., Han, S. ... Wang, Y. (2022). LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway. Experimental and Therapeutic Medicine, 23, 247. https://doi.org/10.3892/etm.2022.11172
MLA
Xu, Y., Shi, H., Wang, M., Huang, P., Xu, M., Han, S., Li, H., Wang, Y."LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway". Experimental and Therapeutic Medicine 23.3 (2022): 247.
Chicago
Xu, Y., Shi, H., Wang, M., Huang, P., Xu, M., Han, S., Li, H., Wang, Y."LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway". Experimental and Therapeutic Medicine 23, no. 3 (2022): 247. https://doi.org/10.3892/etm.2022.11172
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