Cofilin‑1 as a potential biomarker for <em>Mycobacterium tuberculosis</em> infection

Xie,Yiping; Zhang,Zhiqin; Zhang,Min; Cao,Hui

doi:10.3892/etm.2022.11178

Cofilin‑1 as a potential biomarker for Mycobacterium tuberculosis infection

Authors:
- Yiping Xie
- Zhiqin Zhang
- Min Zhang
- Hui Cao
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Affiliations: Department of Clinical Laboratory, Affiliated Kunshan Hospital of Jiangsu University, Kunshan, Jiangsu 215300, P.R. China, Biological Sample Bank, Affiliated Kunshan Hospital of Jiangsu University, Kunshan, Jiangsu 215300, P.R. China, Department of Food Safety and Evaluation, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu 210009, P.R. China
Published online on: February 1, 2022 https://doi.org/10.3892/etm.2022.11178
Article Number: 253
Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tuberculosis (TB) induced by Mycobacterium tuberculosis (M. tb), is one of the deadliest human infections worldwide. Our previous studies demonstrated cofilin‑1 (CFL1) expression was significantly increased in exosomes from Mycobacterium avium (M. avium)‑infected macrophages. The expression of CFL1 protein in M. tb infected hosts was investigated in the present study to predict whether CFL1 could have potential as a biomarker for M. tb infection. In the present study, the mRNA and protein expression levels of CFL1 in M. avium‑infected macrophages and supernatants were analyzed via reverse transcription‑quantitative PCR and western blotting. Furthermore, CFL1 expression in macrophages was knocked down in vivo, and then CFL1 expression levels in M. avium‑infected macrophages and supernatant were detected via western blotting and ELISA. In addition, CFL1 was detected in the peripheral blood mononuclear cells and plasma of patients with TB using western blotting and ELISA. The specificity and sensitivity of CFL1 as a biomarker and the association between TB infection and normal individuals were compared and analyzed using GraphPad Prism 5. CFL1 protein expression levels were significantly increased in M. avium‑infected macrophages and supernatant. Meanwhile, CFL1 was upregulated in patients with TB. Bioinformatics statistics indicated the high specificity and sensitivity of CFL1 in patients with TB. Thus, these results suggest that CFL1 may act as a potential biomarker of TB infection.

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