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Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia‑induced pancreatitis in vitro

  • Authors:
    • Hui-Ying Yang
    • Zhi-Hai Liang
    • Jin-Lian Xie
    • Qing Wu
    • Ying-Ying Qin
    • Shi-Yu Zhang
    • Guo-Du Tang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Gastroenterology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530007, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 290
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    Published online on: February 16, 2022
       https://doi.org/10.3892/etm.2022.11219
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Abstract

an sever actin filaments. Notably, actin dynamics affect the structure and function of epithelial barriers. The present study investigated the role of GSN in the barrier function of pancreatic ductal epithelial cells (PDECs) in hypertriglyceridemia‑induced pancreatitis (HTGP). The human PDEC cell line HPDE6‑C7 underwent GSN knockdown and was treated with caerulein (CAE) + triglycerides (TG). Intracellular calcium levels and the actin filament network were analyzed under a fluorescence microscope. The expression levels of GSN, E‑cadherin, nectin‑2, ZO‑1 and occludin were evaluated by reverse transcription‑quantitative polymerase chain reaction and western blotting. Ultrastructural changes in tight junctions were observed by transmission electron microscopy. Furthermore, the permeability of PDECs was analyzed by fluorescein isothiocyanate‑dextran fluorescence. The results revealed that CAE + TG increased intracellular calcium levels, actin filament depolymerization and GSN expression, and increased PDEC permeability by decreasing the expression levels of E‑cadherin, nectin‑2, ZO‑1 and occludin compared with the control. Moreover, changes in these markers, with the exception of intracellular calcium levels, were reversed by silencing GSN. In conclusion, GSN may disrupt barrier function in PDECs by causing actin filament depolymerization in HTGP in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Yang H, Liang Z, Xie J, Wu Q, Qin Y, Zhang S and Tang G: Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia‑induced pancreatitis <em>in vitro</em>. Exp Ther Med 23: 290, 2022.
APA
Yang, H., Liang, Z., Xie, J., Wu, Q., Qin, Y., Zhang, S., & Tang, G. (2022). Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia‑induced pancreatitis <em>in vitro</em>. Experimental and Therapeutic Medicine, 23, 290. https://doi.org/10.3892/etm.2022.11219
MLA
Yang, H., Liang, Z., Xie, J., Wu, Q., Qin, Y., Zhang, S., Tang, G."Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia‑induced pancreatitis <em>in vitro</em>". Experimental and Therapeutic Medicine 23.4 (2022): 290.
Chicago
Yang, H., Liang, Z., Xie, J., Wu, Q., Qin, Y., Zhang, S., Tang, G."Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia‑induced pancreatitis <em>in vitro</em>". Experimental and Therapeutic Medicine 23, no. 4 (2022): 290. https://doi.org/10.3892/etm.2022.11219
Copy and paste a formatted citation
x
Spandidos Publications style
Yang H, Liang Z, Xie J, Wu Q, Qin Y, Zhang S and Tang G: Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia‑induced pancreatitis <em>in vitro</em>. Exp Ther Med 23: 290, 2022.
APA
Yang, H., Liang, Z., Xie, J., Wu, Q., Qin, Y., Zhang, S., & Tang, G. (2022). Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia‑induced pancreatitis <em>in vitro</em>. Experimental and Therapeutic Medicine, 23, 290. https://doi.org/10.3892/etm.2022.11219
MLA
Yang, H., Liang, Z., Xie, J., Wu, Q., Qin, Y., Zhang, S., Tang, G."Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia‑induced pancreatitis <em>in vitro</em>". Experimental and Therapeutic Medicine 23.4 (2022): 290.
Chicago
Yang, H., Liang, Z., Xie, J., Wu, Q., Qin, Y., Zhang, S., Tang, G."Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia‑induced pancreatitis <em>in vitro</em>". Experimental and Therapeutic Medicine 23, no. 4 (2022): 290. https://doi.org/10.3892/etm.2022.11219
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