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Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression

  • Authors:
    • Qiujing Feng
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    Affiliations: Department of Stomatology, Xingyi People's Hospital, Xingyi, Guizhou 562400, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 296
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    Published online on: February 18, 2022
       https://doi.org/10.3892/etm.2022.11225
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Abstract

Periodontitis is a common inflammatory gum disease that destroys the periodontal tissue. Gastrodin (GAS) is the predominant bioactive component of Gastrodia elata Blume and exhibits anti‑inflammatory, anti‑apoptotic and antioxidant effects in various diseases, including bone‑related diseases. The aim of the present study was to investigate whether GAS could protect lipopolysaccharide (LPS)‑treated human periodontal ligament stem cells (hPDLSCs) against injury and inflammation, and to determine the potential underlying mechanisms. hPDLSCs were treated with LPS and GAS, alone or in combination, and cell viability, inflammation, oxidative stress levels and apoptosis were analyzed using a Cell Counting Kit‑8 assay, ELISA assay, western blotting and flow cytometry, respectively. The osteogenic differentiation capacity was evaluated using an alkaline phosphatase (ALP) assay and Alizarin Red S staining. Sirtuin 3 (SIRT3) was silenced in cells treated with LPS and GAS to verify the involvement of SIRT3 in the effects of GAS. The results demonstrated that LPS‑induced decrease in cell viability was rescued by treatment with 1, 10 or 50 µM GAS. The LPS‑induced production of proinflammatory cytokines and increased level of oxidative stress were also inhibited following treatment with 50 µM GAS. Furthermore, GAS significantly promoted ALP activity, increased the number of mineralized nodules and increased the expression of proteins involved in osteogenic differentiation, including ALP, Runx2, osteocalcin and osteopontin, after osteoinduction, which were all downregulated following LPS stimulation. In addition, GAS prevented LPS‑induced cell apoptosis and restored the imbalance of anti‑apoptotic and proapoptotic proteins in hPDLSCs. In addition, SIRT3 knockdown significantly inhibited the protective effect of GAS on LPS‑induced hPDLSC injury. In summary, the findings form the present study suggested that GAS may protect hPDLSCs from LPS‑induced inflammation, apoptosis and oxidative stress, as well as promote their osteogenic differentiation. The effect of GAS on the osteogenic differentiation of hPDLSCs may therefore depend on the upregulated expression of SIRT3.
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1 

Kumar S: Evidence-based update on diagnosis and management of gingivitis and periodontitis. Dent Clin North Am. 63:69–81. 2019.PubMed/NCBI View Article : Google Scholar

2 

Smith MM, Knight ET, Al-Harthi L and Leichter JW: Chronic periodontitis and implant dentistry. Periodontol 2000. 74:63–73. 2017.PubMed/NCBI View Article : Google Scholar

3 

Hoare A, Soto C, Rojas-Celis V and Bravo D: Chronic inflammation as a link between periodontitis and carcinogenesis. Mediators Inflamm. 2019(1029857)2019.PubMed/NCBI View Article : Google Scholar

4 

Tomokiyo A, Wada N and Maeda H: Periodontal ligament stem cells: Regenerative potency in periodontium. Stem Cells Dev. 28:974–985. 2019.PubMed/NCBI View Article : Google Scholar

5 

Cen LP, Ng TK, Liang JJ, Zhuang X, Yao X, Yam GH, Chen H, Cheung HS, Zhang M and Pang CP: Human periodontal ligament-derived stem cells promote retinal ganglion cell survival and axon regeneration after optic nerve injury. Stem Cells. 36:844–855. 2018.PubMed/NCBI View Article : Google Scholar

6 

Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY and Shi S: Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 364:149–155. 2004.PubMed/NCBI View Article : Google Scholar

7 

Liu Y, Zheng Y, Ding G, Fang D, Zhang C, Bartold PM, Gronthos S, Shi S and Wang S: Periodontal ligament stem cell-mediated treatment for periodontitis in miniature swine. Stem Cells. 26:1065–1073. 2008.PubMed/NCBI View Article : Google Scholar

8 

Zheng W, Wang S, Wang J and Jin F: Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells. Int J Mol Med. 36:915–922. 2015.PubMed/NCBI View Article : Google Scholar

9 

Zhang HS, Liu MF, Ji XY, Jiang CR, Li ZL and OuYang B: Gastrodin combined with rhynchophylline inhibits cerebral ischaemia-induced inflammasome activation via upregulating miR-21-5p and miR-331-5p. Life Sci. 239(116935)2019.PubMed/NCBI View Article : Google Scholar

10 

Sui Y, Bian L, Ai Q, Yao Y, Yu M, Gao H, Zhang A, Fu X, Zhong L and Lu D: Gastrodin inhibits inflammasome through the STAT3 signal pathways in TNA2 astrocytes and reactive astrocytes in experimentally induced cerebral ischemia in rats. Neuromolecular Med. 21:275–286. 2019.PubMed/NCBI View Article : Google Scholar

11 

Qiu CW, Liu ZY, Zhang FL, Zhang L, Li F, Liu SY, He JY and Xiao ZC: Post-stroke gastrodin treatment ameliorates ischemic injury and increases neurogenesis and restores the Wnt/β-catenin signaling in focal cerebral ischemia in mice. Brain Res. 1712:7–15. 2019.PubMed/NCBI View Article : Google Scholar

12 

Liu B, Li F, Shi J, Yang D, Deng Y and Gong Q: Gastrodin ameliorates subacute phase cerebral ischemia-reperfusion injury by inhibiting inflammation and apoptosis in rats. Mol Med Rep. 14:4144–4152. 2016.PubMed/NCBI View Article : Google Scholar

13 

Xi Z, Qiao Y, Wang J, Su H, Bao Z, Li H, Liao X and Zhong X: Gastrodin relieves inflammation injury induced by lipopolysaccharides in MRC-5 cells by up-regulation of miR-103. J Cell Mol Med. 24:1451–1459. 2020.PubMed/NCBI View Article : Google Scholar

14 

Zhang Z, Zhou J, Song D, Sun Y, Liao C and Jiang X: Gastrodin protects against LPS-induced acute lung injury by activating Nrf2 signaling pathway. Oncotarget. 8:32147–32156. 2017.PubMed/NCBI View Article : Google Scholar

15 

Zheng H, Yang E, Peng H, Li J, Chen S, Zhou J, Fang H, Qiu B and Wang Z: Gastrodin prevents steroid-induced osteonecrosis of the femoral head in rats by anti-apoptosis. Chin Med J (Engl). 127:3926–3931. 2014.PubMed/NCBI

16 

Huang Q, Shi J, Gao B, Zhang HY, Fan J, Li XJ, Fan JZ, Han YH, Zhang JK, Yang L, et al: Gastrodin: an ancient Chinese herbal medicine as a source for anti-osteoporosis agents via reducing reactive oxygen species. Bone. 73:132–144. 2015.PubMed/NCBI View Article : Google Scholar

17 

Zhou F, Shen Y, Liu B, Chen X, Wan L and Peng D: Gastrodin inhibits osteoclastogenesis via down-regulating the NFATc1 signaling pathway and stimulates osseointegration in vitro. Biochem Biophys Res Commun. 484:820–826. 2017.PubMed/NCBI View Article : Google Scholar

18 

Liu S, Fang T, Yang L, Chen Z, Mu S and Fu Q: Gastrodin protects MC3T3-E1 osteoblasts from dexamethasone-induced cellular dysfunction and promotes bone formation via induction of the NRF2 signaling pathway. Int J Mol Med. 41:2059–2069. 2018.PubMed/NCBI View Article : Google Scholar

19 

Liu SJ, Liu XY, Li JH, Guo J, Li F, Gui Y, Li XH, Yang L, Wu CY, Yuan Y and Li JJ: Gastrodin attenuates microglia activation through renin-angiotensin system and Sirtuin3 pathway. Neurochem Int. 120:49–63. 2018.PubMed/NCBI View Article : Google Scholar

20 

Yi X, Guo W, Shi Q, Yang Y, Zhang W, Chen X, Kang P, Chen J, Cui T, Ma J, et al: SIRT3-dependent mitochondrial dynamics remodeling contributes to oxidative stress-induced melanocyte degeneration in vitiligo. Theranostics. 9:1614–1633. 2019.PubMed/NCBI View Article : Google Scholar

21 

Giralt A and Villarroya F: SIRT3, a pivotal actor in mitochondrial functions: Metabolism, cell death and aging. Biochem J. 444:1–10. 2012.PubMed/NCBI View Article : Google Scholar

22 

He X, Zeng H and Chen JX: Emerging role of SIRT3 in endothelial metabolism, angiogenesis, and cardiovascular disease. J Cell Physiol. 234:2252–2265. 2019.PubMed/NCBI View Article : Google Scholar

23 

Chen J, Wang A and Chen Q: SirT3 and p53 deacetylation in aging and cancer. J Cell Physiol. 232:2308–2311. 2017.PubMed/NCBI View Article : Google Scholar

24 

Kanwal A, Pillai VB, Samant S, Gupta M and Gupta MP: The nuclear and mitochondrial sirtuins, Sirt6 and Sirt3, regulate each other's activity and protect the heart from developing obesity-mediated diabetic cardiomyopathy. FASEB J. 33:10872–10888. 2019.PubMed/NCBI View Article : Google Scholar

25 

Kitada M, Ogura Y, Monno I and Koya D: Sirtuins and type 2 diabetes: Role in inflammation, oxidative stress, and mitochondrial function. Front Endocrinol (Lausanne). 10(187)2019.PubMed/NCBI View Article : Google Scholar

26 

Hawkes CP and Mostoufi-Moab S: Fat-bone interaction within the bone marrow milieu: Impact on hematopoiesis and systemic energy metabolism. Bone. 119:57–64. 2019.PubMed/NCBI View Article : Google Scholar

27 

Huh JE, Shin JH, Jang ES, Park SJ, Park DR, Ko R, Seo DH, Kim HS, Lee SH, Choi Y, et al: Sirtuin 3 (SIRT3) maintains bone homeostasis by regulating AMPK-PGC-1β axis in mice. Sci Rep. 6(22511)2016.PubMed/NCBI View Article : Google Scholar

28 

Zhao Y, Liu H, Xi X, Chen S and Liu D: TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT. Exp Cell Res. 397(112336)2020.PubMed/NCBI View Article : Google Scholar

29 

Liljestrand JM, Paju S, Buhlin K, Persson GR, Sarna S, Nieminen MS, Sinisalo J, Mäntylä P and Pussinen PJ: Lipopolysaccharide, a possible molecular mediator between periodontitis and coronary artery disease. J Clin Periodontol. 44:784–792. 2017.PubMed/NCBI View Article : Google Scholar

30 

Chen Q, Liu X, Wang D, Zheng J, Chen L, Xie Q, Liu X, Niu S, Qu G, Lan J, et al: Periodontal inflammation-triggered by periodontal ligament stem cell pyroptosis exacerbates periodontitis. Front Cell Dev Biol. 9(663037)2021.PubMed/NCBI View Article : Google Scholar

31 

Nakajima Y, Furuichi Y, Biswas KK, Hashiguchi T, Kawahara K, Yamaji K, Uchimura T, Izumi Y and Maruyama I: Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-kappaB pathway inhibition. FEBS Lett. 580:613–619. 2006.PubMed/NCBI View Article : Google Scholar

32 

Jekabsone A, Sile I, Cochis A, Makrecka-Kuka M, Laucaityte G, Makarova E, Rimondini L, Bernotiene R, Raudone L, Vedlugaite E, et al: Investigation of antibacterial and antiinflammatory activities of proanthocyanidins from pelargonium sidoides DC root extract. Nutrients. 11(2829)2019.PubMed/NCBI View Article : Google Scholar

33 

Liu H, Zheng J, Zheng T and Wang P: Exendin-4 regulates Wnt and NF-κB signaling in lipopolysaccharide-induced human periodontal ligament stem cells to promote osteogenic differentiation. Int Immunopharmacol. 75(105801)2019.PubMed/NCBI View Article : Google Scholar

34 

Zhong JY, Cui RR, Lin X, Xu F, Zhu T, Li F, Wu F, Zhou E, Yi L and Yuan LQ: Aberrant DNA methylation of synaptophysin is involved in adrenal cortisol-producing adenoma. Aging (Albany NY). 11:5232–5245. 2019.PubMed/NCBI View Article : Google Scholar

35 

Liu Y, Gao J, Peng M, Meng H, Ma H, Cai P, Xu Y, Zhao Q and Si G: A review on central nervous system effects of gastrodin. Front Pharmacol. 9(24)2018.PubMed/NCBI View Article : Google Scholar

36 

Zheng B, Shi C, Muhammed FK, He J, Abdullah AO and Liu Y: Gastrodin alleviates bone damage by modulating protein expression and tissue redox state. FEBS Open Bio. 10:2404–2416. 2020.PubMed/NCBI View Article : Google Scholar

37 

Sardiwal S, Magnusson P, Goldsmith DJ and Lamb EJ: Bone alkaline phosphatase in CKD-mineral bone disorder. Am J Kidney Dis. 62:810–822. 2013.PubMed/NCBI View Article : Google Scholar

38 

Yin N, Zhu L, Ding L, Yuan J, Du L, Pan M, Xue F and Xiao H: MiR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells. Cell Mol Biol Lett. 24(51)2019.PubMed/NCBI View Article : Google Scholar

39 

Ding Y, Yang H, Wang Y, Chen J, Ji Z and Sun H: Sirtuin 3 is required for osteogenic differentiation through maintenance of PGC-1α-SOD2-mediated regulation of mitochondrial function. Int J Biol Sci. 13:254–264. 2017.PubMed/NCBI View Article : Google Scholar

40 

Yu X, Meng X, Xu M, Zhang X, Zhang Y, Ding G, Huang S, Zhang A and Jia Z: Celastrol ameliorates cisplatin nephrotoxicity by inhibiting NF-κB and improving mitochondrial function. EBioMedicine. 36:266–280. 2018.PubMed/NCBI View Article : Google Scholar

41 

Tabrizi R, Moosazadeh M, Lankarani KB, Akbari M, Heydari ST, Kolahdooz F and Asemi Z: The effects of synbiotic supplementation on glucose metabolism and lipid profiles in patients with diabetes: A systematic review and meta-analysis of randomized controlled trials. Probiotics Antimicrob Proteins. 10:329–342. 2018.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Feng Q: Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression. Exp Ther Med 23: 296, 2022.
APA
Feng, Q. (2022). Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression. Experimental and Therapeutic Medicine, 23, 296. https://doi.org/10.3892/etm.2022.11225
MLA
Feng, Q."Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression". Experimental and Therapeutic Medicine 23.4 (2022): 296.
Chicago
Feng, Q."Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression". Experimental and Therapeutic Medicine 23, no. 4 (2022): 296. https://doi.org/10.3892/etm.2022.11225
Copy and paste a formatted citation
x
Spandidos Publications style
Feng Q: Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression. Exp Ther Med 23: 296, 2022.
APA
Feng, Q. (2022). Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression. Experimental and Therapeutic Medicine, 23, 296. https://doi.org/10.3892/etm.2022.11225
MLA
Feng, Q."Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression". Experimental and Therapeutic Medicine 23.4 (2022): 296.
Chicago
Feng, Q."Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression". Experimental and Therapeutic Medicine 23, no. 4 (2022): 296. https://doi.org/10.3892/etm.2022.11225
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