Suppression of xenogeneic innate immune response by a membrane‑type human surfactant protein‑A

  • Authors:
    • Chiyoshi Toyama
    • Akira Maeda
    • Shuhei Kogata
    • Riho Yamamoto
    • Kazunori Masahata
    • Takehisa Ueno
    • Masafumi Kamiyama
    • Yuko Tazuke
    • Hiroshi Eguchi
    • Hiroomi Okuyama
    • Shuji Miyagawa
  • View Affiliations

  • Published online on: July 26, 2022     https://doi.org/10.3892/etm.2022.11527
  • Article Number: 590
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Abstract

Macrophage‑mediated xenogeneic rejection is a major immunological obstacle. We recently reported that membrane‑type surfactant protein‑D (SP‑D) on swine endothelial cells (SECs) suppressed macrophage‑mediated rejection. Similar to SP‑D, the carbohydrate recognition domain of surfactant protein‑A (SP‑A) can induce inhibitory signals in effector cells. The present study aimed to examine the suppressive effect of SP‑A on macrophage‑mediated xenogeneic rejection. Naive SECs and SPA‑transfected SECs (SEC/SP‑A) were co‑cultured with THP‑1 cells and cytotoxicity was evaluated. To investigate the effect of SP‑A on phagocytosis, human macrophages were co‑cultured with SEC or SEC/SP‑A, and the extent of phagocytosis and production of reactive oxygen species were assessed via flow cytometry. The mRNA expression levels of inflammatory cytokines in macrophages were determined using reverse transcription‑PCR. Additionally, the effects of THP‑1-Lucia NF‑κB cells on transcription factors were evaluated. The cytotoxicity and phagocytosis of SEC/SP‑A were significantly decreased compared with those of naive SEC. Furthermore, the co‑culture of human macrophages with SEC/SP‑A decreased reactive oxygen species production, and the mRNA expression levels of TNFα were decreased in macrophages, whereas those of IL‑10 were increased. In addition, NF‑κB transcription was decreased in SEC/SP‑A compared with that in SEC. In conclusion, the ectopic expression of human SP‑A in porcine cells represents an attractive method for suppressing macrophage‑mediated cytotoxicity.

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September-2022
Volume 24 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Toyama C, Maeda A, Kogata S, Yamamoto R, Masahata K, Ueno T, Kamiyama M, Tazuke Y, Eguchi H, Okuyama H, Okuyama H, et al: Suppression of xenogeneic innate immune response by a membrane‑type human surfactant protein‑A. Exp Ther Med 24: 590, 2022
APA
Toyama, C., Maeda, A., Kogata, S., Yamamoto, R., Masahata, K., Ueno, T. ... Miyagawa, S. (2022). Suppression of xenogeneic innate immune response by a membrane‑type human surfactant protein‑A. Experimental and Therapeutic Medicine, 24, 590. https://doi.org/10.3892/etm.2022.11527
MLA
Toyama, C., Maeda, A., Kogata, S., Yamamoto, R., Masahata, K., Ueno, T., Kamiyama, M., Tazuke, Y., Eguchi, H., Okuyama, H., Miyagawa, S."Suppression of xenogeneic innate immune response by a membrane‑type human surfactant protein‑A". Experimental and Therapeutic Medicine 24.3 (2022): 590.
Chicago
Toyama, C., Maeda, A., Kogata, S., Yamamoto, R., Masahata, K., Ueno, T., Kamiyama, M., Tazuke, Y., Eguchi, H., Okuyama, H., Miyagawa, S."Suppression of xenogeneic innate immune response by a membrane‑type human surfactant protein‑A". Experimental and Therapeutic Medicine 24, no. 3 (2022): 590. https://doi.org/10.3892/etm.2022.11527