Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2022 Volume 24 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2022 Volume 24 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article

S100A9‑containing serum exosomes obtained from patients with burn injuries promote myocardial cell pyroptosis through NLRP3

  • Authors:
    • Aili Wang
    • Ni Zheng
    • Qing Jia
    • Yu Chen
    • Shun Xu
  • View Affiliations / Copyright

    Affiliations: Department of Burn and Plastic Surgery, The Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China, Department of Nuclear Medicine, The Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China
  • Article Number: 646
    |
    Published online on: September 1, 2022
       https://doi.org/10.3892/etm.2022.11583
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

S100 calcium‑binding protein A9 (S100A9) is highly expressed in the serum exosomes of patients with burn injuries. The present study aimed to investigate the underlying mechanisms of burn injury‑associated exosomes in the regulation of myocardial cell pyroptosis. Reverse transcription‑quantitative PCR and western blotting were used to examine relative mRNA and protein levels. The morphology of exosomes was visualized using transmission electron microscopy. The expression levels of IL‑1β and IL‑18 in cells were examined using ELISA kits. Finally, cell pyroptosis was examined using flow cytometry. When AC16 cells were treated with the serum exosomes obtained from patients with burn injuries, pyroptosis was significantly promoted and the expression levels of IL‑1β and IL‑18 were increased. NLR family pyrin domain containing 3 (NLRP3), S100A9, caspase‑1 and Gasdermin D (GSDMD)‑N expression levels were also upregulated. However, these were significantly reversed by anti‑S100A9 antibodies. Thereafter, CY‑09, an NLRP3 inhibitor, was revealed to restore the increase in pyroptosis and IL‑18, IL‑1β, caspase‑1, NLRP3 and GSDMD‑N expression levels caused by recombinant S100A9 to be similar to the control. These findings suggested that burn injury‑associated exosomes containing S100A9 can affect AC16 cell pyroptosis through NLRP3.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Duan WQ, Xu XW, Cen Y, Xiao HT, Liu XX and Liu Y: Epidemiologic investigation of burn patients in Sichuan Province, China. Med Sci Monit. 25:872–879. 2019.PubMed/NCBI View Article : Google Scholar

2 

Abdullahi A, Amini-Nik S and Jeschke MG: Animal models in burn research. Cell Mol Life Sci. 71:3241–3255. 2014.PubMed/NCBI View Article : Google Scholar

3 

Fozzard HA: Myocardial injury in burn shock. Ann Surg. 154:113–119. 1961.PubMed/NCBI View Article : Google Scholar

4 

Kulp GA, Herndon DN, Lee JO, Suman OE and Jeschke MG: Extent and magnitude of catecholamine surge in pediatric burned patients. Shock. 33:369–374. 2010.PubMed/NCBI View Article : Google Scholar

5 

Gauglitz GG, Herndon DN and Jeschke MG: Insulin resistance postburn: Underlying mechanisms and current therapeutic strategies. J Burn Care Res. 29:683–694. 2008.PubMed/NCBI View Article : Google Scholar

6 

Mecott GA, Al-Mousawi AM, Gauglitz GG, Herndon DN and Jeschke MG: The role of hyperglycemia in burned patients: Evidence-based studies. Shock. 33:5–13. 2010.PubMed/NCBI View Article : Google Scholar

7 

Rivers EP, Katranji M, Jaehne KA, Brown S, Abou Dagher G, Cannon C and Coba V: Early interventions in severe sepsis and septic shock: A review of the evidence one decade later. Minerva Anestesiol. 78:712–724. 2012.PubMed/NCBI

8 

Chen G, Xie M, Dai Z, Wan P, Ye H, Zeng X and Sun Y: Kudingcha and fuzhuan brick tea prevent obesity and modulate gut microbiota in high-fat diet fed mice. Mol Nutr Food Res. 62(e1700485)2018.PubMed/NCBI View Article : Google Scholar

9 

He C, Zheng S, Luo Y and Wang B: Exosome theranostics: Biology and translational medicine. Theranostics. 8:237–255. 2018.PubMed/NCBI View Article : Google Scholar

10 

Zhang Y, Bi J, Huang J, Tang Y, Du S and Li P: Exosome: A review of its classification, isolation techniques, storage, diagnostic and targeted therapy applications. Int J Nanomedicine. 15:6917–6934. 2020.PubMed/NCBI View Article : Google Scholar

11 

Zhang L and Yu D: Exosomes in cancer development, metastasis, and immunity. Biochim Biophys Acta Rev Cancer. 1871:455–468. 2019.PubMed/NCBI View Article : Google Scholar

12 

Li X, Liu L, Yang J, Yu Y, Chai J, Wang L, Ma L and Yin H: Exosome derived from human umbilical cord mesenchymal stem cell mediates MiR-181c attenuating burn-induced excessive inflammation. EBioMedicine. 8:72–82. 2016.PubMed/NCBI View Article : Google Scholar

13 

Qin D, Yang W, Pan Z, Zhang Y, Li X and Lakshmanan S: Differential proteomics analysis of serum exosomein burn patients. Saudi J Biol Sci. 27:2215–2220. 2020.PubMed/NCBI View Article : Google Scholar

14 

Vogl T, Stratis A, Wixler V, Völler T, Thurainayagam S, Jorch SK, Zenker S, Dreiling A, Chakraborty D, Fröhling M, et al: Autoinhibitory regulation of S100A8/S100A9 alarmin activity locally restricts sterile inflammation. J Clin Invest. 128:1852–1866. 2018.PubMed/NCBI View Article : Google Scholar

15 

Idrovo JP, Boe DM, Kaahui S, Yang WL and Kovacs EJ: Hepatic inflammation after burn injury is associated with necroptotic cell death signaling. J Trauma Acute Care Surg. 89:768–774. 2020.PubMed/NCBI View Article : Google Scholar

16 

Fontaine M, Lepape A, Piriou V, Venet F and Friggeri A: Innate danger signals in acute injury: From bench to bedside. Anaesth Crit Care Pain Med. 35:283–292. 2016.PubMed/NCBI View Article : Google Scholar

17 

Xia X, Wang X, Cheng Z, Qin W, Lei L, Jiang J and Hu J: The role of pyroptosis in cancer: pro-cancer or pro-‘host’? Cell Death Dis. 10(650)2019.PubMed/NCBI View Article : Google Scholar

18 

Lin J, Shou X, Mao X, Dong J, Mohabeer N, Kushwaha KK, Wang L, Su Y, Fang H and Li D: Oxidized low density lipoprotein induced caspase-1 mediated pyroptotic cell death in macrophages: Implication in lesion instability? PLoS One. 8(e62148)2013.PubMed/NCBI View Article : Google Scholar

19 

Miao EA, Leaf IA, Treuting PM, Mao DP, Dors M, Sarkar A, Warren SE, Wewers MD and Aderem A: Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria. Nat Immunol. 11:1136–1142. 2010.PubMed/NCBI View Article : Google Scholar

20 

Yuan J, Najafov A and Py BF: Roles of caspases in necrotic cell death. Cell. 167:1693–1704. 2016.PubMed/NCBI View Article : Google Scholar

21 

Miao EA, Rajan JV and Aderem A: Caspase-1-induced pyroptotic cell death. Immunol Rev. 243:206–214. 2011.PubMed/NCBI View Article : Google Scholar

22 

Cookson BT and Brennan MA: Pro-inflammatory programmed cell death. Trends Microbiol. 9:113–114. 2001.PubMed/NCBI View Article : Google Scholar

23 

Franchi L, Muñoz-Planillo R and Núñez G: Sensing and reacting to microbes through the inflammasomes. Nat Immunol. 13:325–332. 2012.PubMed/NCBI View Article : Google Scholar

24 

Martinon F, Mayor A and Tschopp J: The inflammasomes: Guardians of the body. Annu Rev Immunol. 27:229–265. 2009.PubMed/NCBI View Article : Google Scholar

25 

He Y, Hara H and Núñez G: Mechanism and regulation of NLRP3 inflammasome activation. Trends Biochem Sci. 41:1012–1021. 2016.PubMed/NCBI View Article : Google Scholar

26 

Zhu Q, Li Q, Niu X, Zhang G, Ling X, Zhang J, Wang Y and Deng Z: Extracellular vesicles secreted by human urine-derived stem cells promote ischemia repair in a mouse model of hind-limb ischemia. Cell Physiol Biochem. 47:1181–1192. 2018.PubMed/NCBI View Article : Google Scholar

27 

Yin J, Zeng A, Zhang Z, Shi Z, Yan W and You Y: Exosomal transfer of miR-1238 contributes to temozolomide-resistance in glioblastoma. EBioMedicine. 42:238–251. 2019.PubMed/NCBI View Article : Google Scholar

28 

Gritsenko A, Yu S, Martin-Sanchez F, Diaz-Del-Olmo I, Nichols EM, Davis DM, Brough D and Lopez-Castejon G: Priming is dispensable for NLRP3 inflammasome activation in human monocytes in vitro. Front Immunol. 11(565924)2020.PubMed/NCBI View Article : Google Scholar

29 

Kalluri R and LeBleu VS: The biology, function, and biomedical applications of exosomes. Science. 367(eaau6977)2020.PubMed/NCBI View Article : Google Scholar

30 

Wang S, Song R, Wang Z, Jing Z, Wang S and Ma J: S100A8/A9 in inflammation. Front Immunol. 9(1298)2018.PubMed/NCBI View Article : Google Scholar

31 

Valadi H, Ekström K, Bossios A, Sjöstrand M, Lee JJ and Lötvall JO: Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 9:654–659. 2007.PubMed/NCBI View Article : Google Scholar

32 

Emanueli C, Shearn AI, Angelini GD and Sahoo S: Exosomes and exosomal miRNAs in cardiovascular protection and repair. Vascul Pharmacol. 71:24–30. 2015.PubMed/NCBI View Article : Google Scholar

33 

Shi L, Zhao Y, Fei C, Guo J, Jia Y, Wu D, Wu L and Chang C: Cellular senescence induced by S100A9 in mesenchymal stromal cells through NLRP3 inflammasome activation. Aging (Albany NY). 11:9626–9642. 2019.PubMed/NCBI View Article : Google Scholar

34 

Basiorka AA, McGraw KL, Eksioglu EA, Chen X, Johnson J, Zhang L, Zhang Q, Irvine BA, Cluzeau T, Sallman DA, et al: The NLRP3 inflammasome functions as a driver of the myelodysplastic syndrome phenotype. Blood. 128:2960–2975. 2016.PubMed/NCBI View Article : Google Scholar

35 

Viemann D, Barczyk K, Vogl T, Fischer U, Sunderkötter C, Schulze-Osthoff K and Roth J: MRP8/MRP14 impairs endothelial integrity and induces a caspase-dependent and -independent cell death program. Blood. 109:2453–2460. 2007.PubMed/NCBI View Article : Google Scholar

36 

Anceriz N, Vandal K and Tessier PA: S100A9 mediates neutrophil adhesion to fibronectin through activation of beta2 integrins. Biochem Biophys Res Commun. 354:84–89. 2007.PubMed/NCBI View Article : Google Scholar

37 

Ryckman C, Vandal K, Rouleau P, Talbot M and Tessier PA: Proinflammatory activities of S100: Proteins S100A8, S100A9, and S100A8/A9 induce neutrophil chemotaxis and adhesion. J Immunol. 170:3233–3242. 2003.PubMed/NCBI View Article : Google Scholar

38 

Hermani A, Hess J, De Servi B, Medunjanin S, Grobholz R, Trojan L, Angel P and Mayer D: Calcium-binding proteins S100A8 and S100A9 as novel diagnostic markers in human prostate cancer. Clin Cancer Res. 11:5146–5152. 2005.PubMed/NCBI View Article : Google Scholar

39 

Isaacs JT, Dalrymple SL, Rosen DM, Hammers H, Olsson A and Leanderson T: Anti-cancer potency of tasquinimod is enhanced via albumin-binding facilitating increased uptake in the tumor microenvironment. Oncotarget. 5:8093–8106. 2014.PubMed/NCBI View Article : Google Scholar

40 

Schiopu A and Cotoi OS: S100A8 and S100A9: DAMPs at the crossroads between innate immunity, traditional risk factors, and cardiovascular disease. Mediators Inflamm. 2013(828354)2013.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang A, Zheng N, Jia Q, Chen Y and Xu S: S100A9‑containing serum exosomes obtained from patients with burn injuries promote myocardial cell pyroptosis through NLRP3. Exp Ther Med 24: 646, 2022.
APA
Wang, A., Zheng, N., Jia, Q., Chen, Y., & Xu, S. (2022). S100A9‑containing serum exosomes obtained from patients with burn injuries promote myocardial cell pyroptosis through NLRP3. Experimental and Therapeutic Medicine, 24, 646. https://doi.org/10.3892/etm.2022.11583
MLA
Wang, A., Zheng, N., Jia, Q., Chen, Y., Xu, S."S100A9‑containing serum exosomes obtained from patients with burn injuries promote myocardial cell pyroptosis through NLRP3". Experimental and Therapeutic Medicine 24.5 (2022): 646.
Chicago
Wang, A., Zheng, N., Jia, Q., Chen, Y., Xu, S."S100A9‑containing serum exosomes obtained from patients with burn injuries promote myocardial cell pyroptosis through NLRP3". Experimental and Therapeutic Medicine 24, no. 5 (2022): 646. https://doi.org/10.3892/etm.2022.11583
Copy and paste a formatted citation
x
Spandidos Publications style
Wang A, Zheng N, Jia Q, Chen Y and Xu S: S100A9‑containing serum exosomes obtained from patients with burn injuries promote myocardial cell pyroptosis through NLRP3. Exp Ther Med 24: 646, 2022.
APA
Wang, A., Zheng, N., Jia, Q., Chen, Y., & Xu, S. (2022). S100A9‑containing serum exosomes obtained from patients with burn injuries promote myocardial cell pyroptosis through NLRP3. Experimental and Therapeutic Medicine, 24, 646. https://doi.org/10.3892/etm.2022.11583
MLA
Wang, A., Zheng, N., Jia, Q., Chen, Y., Xu, S."S100A9‑containing serum exosomes obtained from patients with burn injuries promote myocardial cell pyroptosis through NLRP3". Experimental and Therapeutic Medicine 24.5 (2022): 646.
Chicago
Wang, A., Zheng, N., Jia, Q., Chen, Y., Xu, S."S100A9‑containing serum exosomes obtained from patients with burn injuries promote myocardial cell pyroptosis through NLRP3". Experimental and Therapeutic Medicine 24, no. 5 (2022): 646. https://doi.org/10.3892/etm.2022.11583
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team