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Porphyromonas gingivalis‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10

  • Authors:
    • Faxiang Zheng
    • Lingkai Su
    • Ning Zhang
    • Longjv Liu
    • Jingyi Gu
    • Wenhua Du
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, Zhejiang 311202, P.R. China, Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China, Department of Stomatology, Emergency General Hospital, Beijing 100028, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 718
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    Published online on: October 14, 2022
       https://doi.org/10.3892/etm.2022.11654
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Abstract

Epidemiological studies have suggested an association between obesity and periodontal disease. Brown adipose tissue (BAT) has an anti‑obesity effect. However, the effects of periodontitis on obesity and BAT remain unclear. Therefore, the present study aimed to determine the effects of lipopolysaccharide derived from Porphyromonas gingivalis (P. gingivalis LPS) on brown adipocytes. For this purpose, the present study examined the effects of the intravenous administration of Porphyromonas gingivalis (P. gingivalis) in mice, the treatment of brown adipocytes with P. gingivalis LPS during differentiation, and the administration of small interfering RNA targeting interferon on brown preadipocytes by assessing the expression of genes involved in differentiation, using a long non‑coding (lnc)RNA, and pro‑inflammatory factors using reverse transcription‑quantitative PCR. In addition, the accumulation of lipid droplets was examined using Oil Red O staining. P. gingivalis LPS reduced the expression of uncoupling protein 1 (UCP1) and lncRNA‑BATE10 in brown adipocytes during differentiation. Consistent with this finding, P. gingivalis reduced UCP1 and lncRNA‑BATE10 expression in the BAT of mice. lncRNA‑BATE10 may thus be involved in the regulation of UCP1 expression that occurs during the differentiation of brown adipocytes treated with P. gingivalis LPS. Thus, P. gingivalis LPS may inhibit BAT differentiation by reducing lncRNA‑BATE10 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng F, Su L, Zhang N, Liu L, Gu J and Du W: <em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10. Exp Ther Med 24: 718, 2022.
APA
Zheng, F., Su, L., Zhang, N., Liu, L., Gu, J., & Du, W. (2022). <em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10. Experimental and Therapeutic Medicine, 24, 718. https://doi.org/10.3892/etm.2022.11654
MLA
Zheng, F., Su, L., Zhang, N., Liu, L., Gu, J., Du, W."<em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10". Experimental and Therapeutic Medicine 24.6 (2022): 718.
Chicago
Zheng, F., Su, L., Zhang, N., Liu, L., Gu, J., Du, W."<em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10". Experimental and Therapeutic Medicine 24, no. 6 (2022): 718. https://doi.org/10.3892/etm.2022.11654
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng F, Su L, Zhang N, Liu L, Gu J and Du W: <em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10. Exp Ther Med 24: 718, 2022.
APA
Zheng, F., Su, L., Zhang, N., Liu, L., Gu, J., & Du, W. (2022). <em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10. Experimental and Therapeutic Medicine, 24, 718. https://doi.org/10.3892/etm.2022.11654
MLA
Zheng, F., Su, L., Zhang, N., Liu, L., Gu, J., Du, W."<em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10". Experimental and Therapeutic Medicine 24.6 (2022): 718.
Chicago
Zheng, F., Su, L., Zhang, N., Liu, L., Gu, J., Du, W."<em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10". Experimental and Therapeutic Medicine 24, no. 6 (2022): 718. https://doi.org/10.3892/etm.2022.11654
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