Open Access

Porphyromonas gingivalis‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10

  • Authors:
    • Faxiang Zheng
    • Lingkai Su
    • Ning Zhang
    • Longjv Liu
    • Jingyi Gu
    • Wenhua Du
  • View Affiliations

  • Published online on: October 14, 2022     https://doi.org/10.3892/etm.2022.11654
  • Article Number: 718
  • Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Epidemiological studies have suggested an association between obesity and periodontal disease. Brown adipose tissue (BAT) has an anti‑obesity effect. However, the effects of periodontitis on obesity and BAT remain unclear. Therefore, the present study aimed to determine the effects of lipopolysaccharide derived from Porphyromonas gingivalis (P. gingivalis LPS) on brown adipocytes. For this purpose, the present study examined the effects of the intravenous administration of Porphyromonas gingivalis (P. gingivalis) in mice, the treatment of brown adipocytes with P. gingivalis LPS during differentiation, and the administration of small interfering RNA targeting interferon on brown preadipocytes by assessing the expression of genes involved in differentiation, using a long non‑coding (lnc)RNA, and pro‑inflammatory factors using reverse transcription‑quantitative PCR. In addition, the accumulation of lipid droplets was examined using Oil Red O staining. P. gingivalis LPS reduced the expression of uncoupling protein 1 (UCP1) and lncRNA‑BATE10 in brown adipocytes during differentiation. Consistent with this finding, P. gingivalis reduced UCP1 and lncRNA‑BATE10 expression in the BAT of mice. lncRNA‑BATE10 may thus be involved in the regulation of UCP1 expression that occurs during the differentiation of brown adipocytes treated with P. gingivalis LPS. Thus, P. gingivalis LPS may inhibit BAT differentiation by reducing lncRNA‑BATE10 expression.
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December-2022
Volume 24 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zheng F, Su L, Zhang N, Liu L, Gu J and Du W: <em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10. Exp Ther Med 24: 718, 2022
APA
Zheng, F., Su, L., Zhang, N., Liu, L., Gu, J., & Du, W. (2022). <em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10. Experimental and Therapeutic Medicine, 24, 718. https://doi.org/10.3892/etm.2022.11654
MLA
Zheng, F., Su, L., Zhang, N., Liu, L., Gu, J., Du, W."<em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10". Experimental and Therapeutic Medicine 24.6 (2022): 718.
Chicago
Zheng, F., Su, L., Zhang, N., Liu, L., Gu, J., Du, W."<em>Porphyromonas</em> <em>gingivalis</em>‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10". Experimental and Therapeutic Medicine 24, no. 6 (2022): 718. https://doi.org/10.3892/etm.2022.11654