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Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice

  • Authors:
    • Xing-Chen Dai
    • Yu-Huan Zhang
    • Yong-Li Huang
    • Xiao-Ting Wu
    • Yu-Jie Fang
    • Yu-Jing Gao
    • Fang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, General Hospital, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
    Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 59
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    Published online on: December 8, 2022
       https://doi.org/10.3892/etm.2022.11758
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Abstract

Colorectal cancer (CRC) is one of the most common cancers worldwide and the consumption of a high‑calorie diet is one of its risk factors. Calorie restriction (CR) slows tumor growth in a variety of cancers, including colorectal cancer; however, the mechanism behind this remains unknown. In the present study, CR effectively reduced the tumor volume and weight in a xenograft BALB/c male nude mouse model. In addition, tumor immunohistochemistry revealed that the CR group had significantly higher expression of Bax (P<0.001) and significantly lower levels of Bcl2 (P<0.0001) and Ki67 (P<0.001) compared with control group. Furthermore, data from 16S ribosomal (r)RNA sequencing implied that CR was able to reprogram the microbiota structure, characterized by increased Lactobacillus constituent ratio (P<0.05), with amelioration of microbial dysbiosis caused by CRC. Further receiver operating characteristic curves demonstrated that the bacteria Bacteroides [area under the curve (AUC)=0.800], Lactobacillus (AUC=0.760) and Roseburia (AUC=0.720) served key roles in suppression of CRC in the mouse model. The functional prediction of intestinal flora indicated ‘cyanoamino acid metabolism’ (P<0.01), ‘replication initiation protein REP (rolling circle plasmid replication)’ (P<0.01), ‘tRNA G10 N‑methylase Trm11’ (P<0.01) and ‘uncharacterized protein with cyclophilin fold, contains DUF369 domain’ (P<0.05) were downregulated in CR group. These findings implied that CR suppressed CRC in mice and altered the gut microbiota.
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Copy and paste a formatted citation
Spandidos Publications style
Dai X, Zhang Y, Huang Y, Wu X, Fang Y, Gao Y and Wang F: Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice. Exp Ther Med 25: 59, 2023.
APA
Dai, X., Zhang, Y., Huang, Y., Wu, X., Fang, Y., Gao, Y., & Wang, F. (2023). Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice. Experimental and Therapeutic Medicine, 25, 59. https://doi.org/10.3892/etm.2022.11758
MLA
Dai, X., Zhang, Y., Huang, Y., Wu, X., Fang, Y., Gao, Y., Wang, F."Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice". Experimental and Therapeutic Medicine 25.1 (2023): 59.
Chicago
Dai, X., Zhang, Y., Huang, Y., Wu, X., Fang, Y., Gao, Y., Wang, F."Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice". Experimental and Therapeutic Medicine 25, no. 1 (2023): 59. https://doi.org/10.3892/etm.2022.11758
Copy and paste a formatted citation
x
Spandidos Publications style
Dai X, Zhang Y, Huang Y, Wu X, Fang Y, Gao Y and Wang F: Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice. Exp Ther Med 25: 59, 2023.
APA
Dai, X., Zhang, Y., Huang, Y., Wu, X., Fang, Y., Gao, Y., & Wang, F. (2023). Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice. Experimental and Therapeutic Medicine, 25, 59. https://doi.org/10.3892/etm.2022.11758
MLA
Dai, X., Zhang, Y., Huang, Y., Wu, X., Fang, Y., Gao, Y., Wang, F."Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice". Experimental and Therapeutic Medicine 25.1 (2023): 59.
Chicago
Dai, X., Zhang, Y., Huang, Y., Wu, X., Fang, Y., Gao, Y., Wang, F."Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice". Experimental and Therapeutic Medicine 25, no. 1 (2023): 59. https://doi.org/10.3892/etm.2022.11758
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