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P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation

  • Authors:
    • Yuqiong Zhao
    • Xiaojie Ren
    • Fan Li
    • Binghan Jia
    • Dengke Wang
    • Hua Jia
    • Xuwen Jiao
    • Lixin Wang
    • Junping Li
  • View Affiliations / Copyright

    Affiliations: Department of Human Anatomy and Histoembryology, College of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750001, P.R. China, Department of Gastrointestinal Surgery, Xiantao First People's Hospital Affiliated to Yangtze University, Xiantao, Hubei 433000, P.R. China, The Medical Laboratory Center of General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750001, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 67
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    Published online on: December 13, 2022
       https://doi.org/10.3892/etm.2022.11766
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Abstract

The aim of the present study was to explore the expression changes of P2Y purinergic receptor 1 (P2Y1) in the distal colonic submucosa of opioid‑induced constipation (OIC) rats and its association with the occurrence of OIC, an OIC rat model was generated by intraperitoneal injection of loperamide hydrochloride, a selective agonist of µ‑opioid receptors (MORs). At 7 days post‑treatment, the model was assessed by analyzing stool scores and calculating the gastrointestinal (GI) transit ratio of rats. The distribution of P2Y1‑expressing neurons in the colonic submucosal plexus was demonstrated by immunofluorescence (IF). Western blotting was performed to evaluate the expression changes of MOR, P2Y1 and ATP synthase subunit β (ATPB) proteins in the colonic submucosa, while reverse transcription‑quantitative PCR (RT‑qPCR) analysis was performed to determine the relative mRNA expression of MOR and P2Y1. After 7 days, the feces of OIC rats exhibited an appearance of sausage‑shaped pieces and both the stool weight and GI transit ratio of OIC rats were significantly decreased. IF revealed co‑expression of P2Y1 and calbindin and MOR and ATPB in the nerve cells of the distal colonic submucosal plexus. Moreover, RT‑qPCR analysis showed that the MOR mRNA levels were significantly increased in the distal colonic submucosa of OIC rats, while mRNA levels of P2Y1 were decreased. WB showed that in the distal colonic submucosa of OIC rats, MOR protein expression was increased, whereas that of P2Y1 was significantly decreased. GI transit ratio analysis suggested that the P2Y agonist ATP significantly relieved constipation symptoms in rats, while the P2Y inhibitor MRS2179 aggravated these symptoms. Finally, P2Y1 expression change was shown to be associated with the occurrence of OIC, while expression of MOR and P2Y1 was associated with OIC development in rats.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y, Ren X, Li F, Jia B, Wang D, Jia H, Jiao X, Wang L and Li J: P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation. Exp Ther Med 25: 67, 2023.
APA
Zhao, Y., Ren, X., Li, F., Jia, B., Wang, D., Jia, H. ... Li, J. (2023). P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation. Experimental and Therapeutic Medicine, 25, 67. https://doi.org/10.3892/etm.2022.11766
MLA
Zhao, Y., Ren, X., Li, F., Jia, B., Wang, D., Jia, H., Jiao, X., Wang, L., Li, J."P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation". Experimental and Therapeutic Medicine 25.1 (2023): 67.
Chicago
Zhao, Y., Ren, X., Li, F., Jia, B., Wang, D., Jia, H., Jiao, X., Wang, L., Li, J."P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation". Experimental and Therapeutic Medicine 25, no. 1 (2023): 67. https://doi.org/10.3892/etm.2022.11766
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y, Ren X, Li F, Jia B, Wang D, Jia H, Jiao X, Wang L and Li J: P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation. Exp Ther Med 25: 67, 2023.
APA
Zhao, Y., Ren, X., Li, F., Jia, B., Wang, D., Jia, H. ... Li, J. (2023). P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation. Experimental and Therapeutic Medicine, 25, 67. https://doi.org/10.3892/etm.2022.11766
MLA
Zhao, Y., Ren, X., Li, F., Jia, B., Wang, D., Jia, H., Jiao, X., Wang, L., Li, J."P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation". Experimental and Therapeutic Medicine 25.1 (2023): 67.
Chicago
Zhao, Y., Ren, X., Li, F., Jia, B., Wang, D., Jia, H., Jiao, X., Wang, L., Li, J."P2Y1 receptor in the colonic submucosa of rats and its association with opioid‑induced constipation". Experimental and Therapeutic Medicine 25, no. 1 (2023): 67. https://doi.org/10.3892/etm.2022.11766
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