Open Access

Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway

  • Authors:
    • Haoyu Yuan
    • Huacheng Sui
    • Saimei Li
  • View Affiliations

  • Published online on: April 18, 2023     https://doi.org/10.3892/etm.2023.11958
  • Article Number: 259
  • Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diabetic nephropathy (DN) is the predominant cause of end‑stage renal disease globally. Diosgenin (DSG) has been reported to play a protective role in podocyte injury in DN. The present study aimed to explore the role of DSG in DN, as well as its mechanism of action in a high glucose (HG)‑induced in vitro model of DN in podocytes. Cell viability, apoptosis, inflammatory response and insulin‑stimulated glucose uptake were evaluated using Cell Counting Kit‑8, TUNEL, ELISA and 2‑deoxy‑D‑glucose assay, respectively. In addition, the expression of AMP‑activated protein kinase (AMPK)/sirtuin 1 (SIRT1)/NF‑κB signaling‑related proteins in podocyte cells was measured using western blotting. The results indicated that DSG enhanced the viability of podocytes after HG exposure, but inhibited inflammatory damage and attenuated insulin resistance. Moreover, DSG induced the activation of the AMPK/SIRT1/NF‑κB signaling pathway. Furthermore, treatment with compound C, an inhibitor of AMPK, counteracted the protective effects of DSG on HG‑induced podocyte cells. Therefore, DSG may be a potential therapeutic compound for the treatment of diabetic nephropathy.
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June-2023
Volume 25 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yuan H, Sui H and Li S: Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway. Exp Ther Med 25: 259, 2023
APA
Yuan, H., Sui, H., & Li, S. (2023). Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 25, 259. https://doi.org/10.3892/etm.2023.11958
MLA
Yuan, H., Sui, H., Li, S."Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 25.6 (2023): 259.
Chicago
Yuan, H., Sui, H., Li, S."Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 25, no. 6 (2023): 259. https://doi.org/10.3892/etm.2023.11958