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Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway

  • Authors:
    • Haoyu Yuan
    • Huacheng Sui
    • Saimei Li
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The First Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 259
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    Published online on: April 18, 2023
       https://doi.org/10.3892/etm.2023.11958
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Abstract

Diabetic nephropathy (DN) is the predominant cause of end‑stage renal disease globally. Diosgenin (DSG) has been reported to play a protective role in podocyte injury in DN. The present study aimed to explore the role of DSG in DN, as well as its mechanism of action in a high glucose (HG)‑induced in vitro model of DN in podocytes. Cell viability, apoptosis, inflammatory response and insulin‑stimulated glucose uptake were evaluated using Cell Counting Kit‑8, TUNEL, ELISA and 2‑deoxy‑D‑glucose assay, respectively. In addition, the expression of AMP‑activated protein kinase (AMPK)/sirtuin 1 (SIRT1)/NF‑κB signaling‑related proteins in podocyte cells was measured using western blotting. The results indicated that DSG enhanced the viability of podocytes after HG exposure, but inhibited inflammatory damage and attenuated insulin resistance. Moreover, DSG induced the activation of the AMPK/SIRT1/NF‑κB signaling pathway. Furthermore, treatment with compound C, an inhibitor of AMPK, counteracted the protective effects of DSG on HG‑induced podocyte cells. Therefore, DSG may be a potential therapeutic compound for the treatment of diabetic nephropathy.
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1 

Heerspink HJL, Parving HH, Andress DL, Bakris G, Correa-Rotter R, Hou FF, Kitzman DW, Kohan D, Makino H, McMurray JJV, et al: Atrasentan and renal events in patients with type 2 diabetes and chronic kidney disease (SONAR): A double-blind, randomised, placebo-controlled trial. Lancet. 393:1937–1947. 2019.PubMed/NCBI View Article : Google Scholar

2 

Bell S, Fletcher EH, Brady I, Looker HC, Levin D, Joss N, Traynor JP, Metcalfe W, Conway B, Livingstone S, et al: End-stage renal disease and survival in people with diabetes: A national database linkage study. QJM. 108:127–134. 2015.PubMed/NCBI View Article : Google Scholar

3 

Verzola D, Cappuccino L, D'Amato E, Villaggio B, Gianiorio F, Mij M, Simonato A, Viazzi F, Salvidio G and Garibotto F: Enhanced glomerular Toll-like receptor 4 expression and signaling in patients with type 2 diabetic nephropathy and microalbuminuria. Kidney Int. 86:1229–1243. 2014.PubMed/NCBI View Article : Google Scholar

4 

Rossing P and Persson F: What have we learned so far from the use of sodium-glucose cotransporter 2 inhibitors in clinical practice? Adv Chronic Kidney Dis. 28:290–297. 2021.PubMed/NCBI View Article : Google Scholar

5 

Zhu X, Tang L, Mao J, Hameed Y, Zhang J, Li N, Wu D, Huang Y and Li C: Decoding the Mechanism behind the pathogenesis of the focal segmental glomerulosclerosis. Comput Math Methods Med. 2022(1941038)2022.PubMed/NCBI View Article : Google Scholar

6 

Tung CW, Hsu YC, Shih YH, Chang PJ and Lin CL: Glomerular mesangial cell and podocyte injuries in diabetic nephropathy. Nephrology (Carlton). 23 (Suppl 4):S32–S37. 2018.PubMed/NCBI View Article : Google Scholar

7 

Pi WX, Feng XP, Ye LH and Cai BC: Combination of morroniside and diosgenin prevents high glucose-induced cardiomyocytes apoptosis. Molecules. 22(163)2017.PubMed/NCBI View Article : Google Scholar

8 

Yang WS, Moon SY, Lee MJ, Lee EK and Park SK: Diosgenin, an activator of 1,25D3-MARRS receptor/ERp57, attenuates the effects of TNF-α by causing ADAM10-dependent ectodomain shedding of TNF receptor 1. Cell Physiol Biochem. 43:2434–2445. 2017.PubMed/NCBI View Article : Google Scholar

9 

Long C, Chen J, Zhou H, Jiang T, Fang X, Hou D, Liu P and Duan H: Diosgenin exerts its tumor suppressive function via inhibition of Cdc20 in osteosarcoma cells. Cell Cycle. 18:346–358. 2019.PubMed/NCBI View Article : Google Scholar

10 

Kalailingam P, Kannaian B, Tamilmani E and Kaliaperumal R: Efficacy of natural diosgenin on cardiovascular risk, insulin secretion, and beta cells in streptozotocin (STZ)-induced diabetic rats. Phytomedicine. 21:1154–1161. 2014.PubMed/NCBI View Article : Google Scholar

11 

Hua S, Li Y, Su L and Liu X: Diosgenin ameliorates gestational diabetes through inhibition of sterol regulatory element-binding protein-1. Biomed Pharmacother. 84:1460–1465. 2016.PubMed/NCBI View Article : Google Scholar

12 

Chiang SS, Chang SP and Pan TM: Osteoprotective effect of Monascus-fermented dioscorea in ovariectomized rat model of postmenopausal osteoporosis. J Agric Food Chem. 59:9150–9157. 2011.PubMed/NCBI View Article : Google Scholar

13 

Gan Q, Wang J, Hu J, Lou G, Xiong H, Peng C, Zheng S and Huang Q: The role of diosgenin in diabetes and diabetic complications. J Steroid Biochem Mol Biol. 198(105575)2020.PubMed/NCBI View Article : Google Scholar

14 

Wang Z, Wu Q, Wang H, Gao Y, Nie K, Tang Y, Su H, Hu M, Gong J, Fang K and Dong H: Diosgenin protects against podocyte injury in early phase of diabetic nephropathy through regulating SIRT6. Phytomedicine. 104(154276)2022.PubMed/NCBI View Article : Google Scholar

15 

Xue W, Mao J, Chen Q, Ling W and Sun Y: Mogroside IIIE alleviates high glucose-induced inflammation, oxidative stress and apoptosis of podocytes by the activation of AMPK/SIRT1 signaling pathway. Diabetes Metab Syndr Obes. 13:3821–3830. 2020.PubMed/NCBI View Article : Google Scholar

16 

Chen B, Li J and Zhu H: AMP-activated protein kinase attenuates oxLDL uptake in macrophages through PP2A/NF-κB/LOX-1 pathway. Vascul Pharmacol. 85:1–10. 2016.PubMed/NCBI View Article : Google Scholar

17 

Li F, Chen Y, Li Y, Huang M and Zhao W: Geniposide alleviates diabetic nephropathy of mice through AMPK/SIRT1/NF-kappaB pathway. Eur J Pharmacol. 886(173449)2020.PubMed/NCBI View Article : Google Scholar

18 

Chen Y, Xu X, Zhang Y, Liu K, Huang F, Liu B and Kou J: Diosgenin regulates adipokine expression in perivascular adipose tissue and ameliorates endothelial dysfunction via regulation of AMPK. J Steroid Biochem Mol Biol. 155:155–165. 2016.PubMed/NCBI View Article : Google Scholar

19 

Samsu N: Diabetic nephropathy: Challenges in pathogenesis, diagnosis, and treatment. Biomed Res Int. 2021(1497449)2021.PubMed/NCBI View Article : Google Scholar

20 

Li F, Dai B and Ni X: Long non-coding RNA cancer susceptibility candidate 2 (CASC2) alleviates the high glucose-induced injury of CIHP-1 cells via regulating miR-9-5p/PPARγ axis in diabetes nephropathy. Diabetol Metab Syndr. 12(68)2020.PubMed/NCBI View Article : Google Scholar

21 

Yang F, Qin Y, Wang Y, Meng S, Xian H, Che H, Lv J, Li Y, Yu Y, Bai Y and Wang L: Metformin Inhibits the NLRP3 inflammasome via AMPK/mTOR-dependent effects in diabetic cardiomyopathy. Int J Biol Sci. 15:1010–1019. 2019.PubMed/NCBI View Article : Google Scholar

22 

Kumar P, Nagarajan A and Uchil PD: Analysis of cell viability by the lactate dehydrogenase assay. Cold Spring Harb Protoc 2018: doi: 10.1101/pdb.prot095497, 2018.

23 

Zhan X, Yan C, Chen Y, Wei X, Xiao J, Deng L, Yang Y, Qiu P and Chen Q: Celastrol antagonizes high glucose-evoked podocyte injury, inflammation and insulin resistance by restoring the HO-1-mediated autophagy pathway. Mol Immunol. 104:61–68. 2018.PubMed/NCBI View Article : Google Scholar

24 

Li Y, Li Y, Yang T and Wang M: Dioscin attenuates oxLDL uptake and the inflammatory reaction of dendritic cells under high glucose conditions by blocking p38 MAPK. Mol Med Rep. 21:304–310. 2020.PubMed/NCBI View Article : Google Scholar

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

26 

Li G, Huang D, Li N, Ritter JK and Li PL: Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes. Redox Biol. 43(102013)2021.PubMed/NCBI View Article : Google Scholar

27 

Wu S, Zhao M, Sun Y, Xie M, Le K, Xu M and Huang C: The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model. Life Sci. 241(117115)2020.PubMed/NCBI View Article : Google Scholar

28 

Du S, Li C, Lu Y, Lei X, Zhang Y, Li S, Liu F, Chen Y, Weng D and Chen J: Dioscin alleviates crystalline silica-induced pulmonary inflammation and fibrosis through promoting alveolar macrophage autophagy. Theranostics. 9:1878–1892. 2019.PubMed/NCBI View Article : Google Scholar

29 

Khosravi Z, Sedaghat R, Baluchnejadmojarad T and Roghani M: Diosgenin ameliorates testicular damage in streptozotocin-diabetic rats through attenuation of apoptosis, oxidative stress, and inflammation. Int Immunopharmacol. 70:37–46. 2019.PubMed/NCBI View Article : Google Scholar

30 

Opazo-Rios L, Mas S, Marin-Royo G, Mezzano S, Gómez-Guerrero C, Moreno JA and Egido J: Lipotoxicity and diabetic nephropathy: Novel mechanistic insights and therapeutic opportunities. Int J Mol Sci. 21(2632)2020.PubMed/NCBI View Article : Google Scholar

31 

Liu K, Zhao W, Gao X, Huang F, Kou J and Liu B: Diosgenin ameliorates palmitate-induced endothelial dysfunction and insulin resistance via blocking IKKβ and IRS-1 pathways. Atherosclerosis. 223:350–358. 2012.PubMed/NCBI View Article : Google Scholar

32 

Naidu PB, Ponmurugan P, Begum MS, Mohan K, Meriga B, RavindarNaik R and Saravanan G: Diosgenin reorganises hyperglycaemia and distorted tissue lipid profile in high-fat diet-streptozotocin-induced diabetic rats. J Sci Food Agric. 95:3177–3182. 2015.PubMed/NCBI View Article : Google Scholar

33 

Lu J, Chen PP, Zhang JX, Li XQ, Wang GH, Yuan BY, Huang SJ, Liu XQ, Jiang TT, Wang MY, et al: GPR43 deficiency protects against podocyte insulin resistance in diabetic nephropathy through the restoration of AMPKalpha activity. Theranostics. 11:4728–4742. 2021.PubMed/NCBI View Article : Google Scholar

34 

Ran X, Yan Z, Yang Y, Hu G, Liu J, Hou S, Guo W, Kan X and Fu S: Dioscin improves pyroptosis in LPS-induced mice mastitis by activating AMPK/Nrf2 and inhibiting the NF-kappaB signaling pathway. Oxid Med Cell Longev. 2020(8845521)2020.PubMed/NCBI View Article : Google Scholar

35 

Yao H, Tao X, Xu L, Qi Y, Yin L, Han X, Xu Y, Zheng L and Peng J: Dioscin alleviates non-alcoholic fatty liver disease through adjusting lipid metabolism via SIRT1/AMPK signaling pathway. Pharmacol Res. 131:51–60. 2018.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Yuan H, Sui H and Li S: Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway. Exp Ther Med 25: 259, 2023.
APA
Yuan, H., Sui, H., & Li, S. (2023). Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 25, 259. https://doi.org/10.3892/etm.2023.11958
MLA
Yuan, H., Sui, H., Li, S."Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 25.6 (2023): 259.
Chicago
Yuan, H., Sui, H., Li, S."Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 25, no. 6 (2023): 259. https://doi.org/10.3892/etm.2023.11958
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan H, Sui H and Li S: Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway. Exp Ther Med 25: 259, 2023.
APA
Yuan, H., Sui, H., & Li, S. (2023). Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 25, 259. https://doi.org/10.3892/etm.2023.11958
MLA
Yuan, H., Sui, H., Li, S."Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 25.6 (2023): 259.
Chicago
Yuan, H., Sui, H., Li, S."Diosgenin alleviates the inflammatory damage and insulin resistance in high glucose‑induced podocyte cells via the AMPK/SIRT1/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 25, no. 6 (2023): 259. https://doi.org/10.3892/etm.2023.11958
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