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Article

Methylation haplotypes of the insulin gene promoter in children and adolescents with type 1 diabetes: Can a dimensionality reduction approach predict the disease?

  • Authors:
    • Eleni P. Kotanidou
    • Alexandra Kosvyra
    • Konstantina Mouzaki
    • Styliani Giza
    • Vasiliki Rengina Tsinopoulou
    • Anastasios Serbis
    • Ioanna Chouvarda
    • Assimina Galli‑Tsinopoulou
  • View Affiliations / Copyright

    Affiliations: Second Department of Pediatrics, Unit of Pediatric Endocrinology and Metabolism, Faculty of Health Sciences, School of Medicine, Aristotle University of Thessaloniki, AHEPA University Hospital, 54636 Thessaloniki, Greece, Laboratory of Computing, Medical Informatics and Biomedical Imaging Technologies, Faculty of Health Sciences, School of Medicine, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece
  • Article Number: 461
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    Published online on: August 8, 2023
       https://doi.org/10.3892/etm.2023.12160
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Abstract

DNA methylation of cytosine‑guanine sites (CpGs) is associated with type 1 diabetes (T1D). The sequence of methylated and non‑methylated sites in a specific genetic region constitutes its methyl‑haplotype. The aim of the present study was to identify insulin gene promoter (IGP) methyl‑haplotypes among children and adolescents with T1D and suggest a predictive model for the discrimination of cases and controls according to methyl‑haplotypes. A total of 40 individuals (20 T1D) participated. The IGP region from peripheral whole blood DNA of 40 participants (20 T1D) was sequenced using next‑generation sequencing, sequences were read using FASTQ files and methylation status was calculated by python‑based pipeline for targeted deep bisulfite sequenced amplicons (ampliMethProfiler). Methylation profile at 10 CpG sites proximal to transcription start site of the IGP was recorded and coded as 0 for unmethylation or 1 for methylation. A single read could result in ‘1111111111’ methyl‑haplotype (all methylated), ‘000000000’ methyl‑haplotype (all unmethylated) or any other combination. Principal component analysis was applied to the generated methyl‑haplotypes for dimensionality reduction, and the first three principal components were employed as features with five different classifiers (random forest, decision tree, logistic regression, Naive Bayes, support vector machine). Naive Bayes was the best‑performing classifier, with 0.9 accuracy. Predictive models were evaluated using receiver operating characteristics (AUC 0.96). Methyl‑haplotypes ‘1111111111’, ‘1111111011’, ‘1110111111’, ‘1111101111’ and ‘1110101111’ were revealed to be the most significantly associated with T1D according to the dimensionality reduction method. Methylation‑based biomarkers such as IGP methyl‑haplotypes could serve to identify individuals at high risk for T1D.
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View References

1 

Jerram ST, Dang MN and Leslie RD: The role of epigenetics in type 1 diabetes. Curr Diab Rep. 17(89)2017.PubMed/NCBI View Article : Google Scholar

2 

Xie Z, Chang C, Huang G and Zhou Z: The role of epigenetics in type 1 diabetes. Adv Exp Med Biol. 1253:223–257. 2020.PubMed/NCBI View Article : Google Scholar

3 

Allis CD and Jenuwein T: The molecular hallmarks of epigenetic control. Nat Rev Genet. 17:487–500. 2016.PubMed/NCBI View Article : Google Scholar

4 

Bird A: Perceptions of epigenetics. Nature. 447:396–398. 2007.PubMed/NCBI View Article : Google Scholar

5 

D'Angeli MA, Merzon E, Valbuena LF, Tirschwell D, Paris CA and Mueller BA: Environmental factors associated with childhood-onset type 1 diabetes mellitus: An exploration of the hygiene and overload hypotheses. Arch Pediatr Adolesc Med. 164:732–738. 2010.PubMed/NCBI View Article : Google Scholar

6 

Kotanidou EP, Mouzaki K, Chouvarda I, Koutsiana E, Kosvyra A, Tsinopoulou VR, Giza S, Serbis A and Galli-Tsinopoulou A: CpG methylation haplotypes of the insulin gene promoter as predictive biomarker in a cohort of children and adolescents with type 1 diabetes. Paediatriki. 83:150–162. 2021.

7 

Steck AK and Rewers MJ: Genetics of type 1 diabetes. Clin Chem. 57:176–185. 2011.

8 

Moulder R, Bhosale SD, Erkkilä T, Laajala E, Salmi J, Nguyen EV, Kallionpää H, Mykkänen J, Vähä-Mäkilä M, Hyöty H, et al: Serum proteomes distinguish children developing type 1 diabetes in a cohort with HLA-conferred susceptibility. Diabetes. 64:2265–2278. 2015.PubMed/NCBI View Article : Google Scholar

9 

Rodríguez-Ventura AL, Yamamoto-Furusho JK, Coyote N, Dorantes LM, Ruiz-Morales JA, Vargas-Alarcón G and Granados J: HLA-DRB1*08 allele may help to distinguish between type 1 diabetes mellitus and type 2 diabetes mellitus in Mexican children. Pediatr Diabetes. 8:5–10. 2007.PubMed/NCBI View Article : Google Scholar

10 

Kavakiotis I, Tsave O, Salifoglou A, Maglaveras N, Vlahavas I and Chouvarda I: Machine learning and data mining methods in diabetes research. Comput Struct Biotechnol J. 15:104–116. 2017.PubMed/NCBI View Article : Google Scholar

11 

Zhang H and Pollin TI: Epigenetics variation and pathogenesis in diabetes. Curr Diab Rep. 18(121)2018.PubMed/NCBI View Article : Google Scholar

12 

Li J, Ding J, Zhi DU, Gu K and Wang H: Identification of type 2 diabetes based on a ten-gene biomarker prediction model constructed using a support vector machine algorithm. Biomed Res Int. 2022(1230761)2022.PubMed/NCBI View Article : Google Scholar

13 

Lethebe BC, Williamson T, Garies S, McBrien K, Leduc C, Butalia S, Soos B, Shaw M and Drummond N: Developing a case definition for type 1 diabetes mellitus in a primary care electronic medical record database: An exploratory study. CMAJ Open. 7:E246–E251. 2019.PubMed/NCBI View Article : Google Scholar

14 

Fufurin I, Berezhanskiy P, Golyak I, Anfimov D, Kareva E, Scherbakova A, Demkin P, Nebritova O and Morozov A: Deep learning for type 1 diabetes mellitus diagnosis using infrared quantum cascade laser spectroscopy. Materials (Basel). 15(2984)2022.PubMed/NCBI View Article : Google Scholar

15 

Seoighe C, Tosh NJ and Greally JM: DNA methylation haplotypes as cancer markers. Nat Genet. 50:1062–1063. 2018.PubMed/NCBI View Article : Google Scholar

16 

Jiang P, Wei K, Xu L, Chang C, Zhang R, Zhao J, Jin Y, Xu L, Shi Y, Qian Y, et al: DNA methylation change of HIPK3 in Chinese rheumatoid arthritis and its effect on inflammation. Front Immunol. 13(1087279)2023.PubMed/NCBI View Article : Google Scholar

17 

Fradin D, Le Fur S, Mille C, Naoui N, Groves C, Zelenika D, McCarthy MI, Lathrop M and Bougnères P: Association of the CpG methylation pattern of the proximal insulin gene promoter with type 1 diabetes. PLoS One. 7(e36278)2012.PubMed/NCBI View Article : Google Scholar

18 

ElSayed NA, Aleppo G, Aroda VR, Bannuru RR, Brown FM, Bruemmer D, Collins BS, Hilliard ME, Isaacs D, Johnson EL, et al: Classification and diagnosis of diabetes: Standards of care in diabetes-2023. Diabetes Care. 46:19–40. 2023.PubMed/NCBI View Article : Google Scholar

19 

Libman I, Haynes A, Lyons S, Pradeep P, Rwagasor E, Tung JY, Jefferies CA, Oram RA, Dabelea D and Craig ME: ISPAD clinical practice consensus guidelines 2022: Definition, epidemiology, and classification of diabetes in children and adolescents. Pediatr Diabetes. 23:1160–1174. 2022.PubMed/NCBI View Article : Google Scholar

20 

Scala G, Affinito O, Palumbo D, Florio E, Monticelli A, Miele G, Chiariotti L and Cocozza S: AmpliMethProfiler: A pipeline for the analysis of CpG methylation profiles of targeted deep bisulfite sequenced amplicons. BMC Bioinformatics. 17(484)2016.PubMed/NCBI View Article : Google Scholar

21 

von Luxburg U: A tutorial on spectral clustering. Stat Comput. 17:395–416. 2007.

22 

Jollife IT and Cadima J: Principal component analysis: A review and recent developments. Philos Trans A Math Physc Eng Sci. 374(20150202)2016.PubMed/NCBI View Article : Google Scholar

23 

Pedregosa F, Varoquaux G, Gramfort A, Michel V, Thirion B, Grisel O, Blondel M, Prettenhofer P, Weiss R, Dubourg V, et al: Scikit-learn: Machine learning in python. J Mach Learn Res. 12:2825–2830. 2011.

24 

Frost HR: Eigenvectors from eigenvalues Sparse principal component analysis (EESPCA). J Comput Graph Stat. 31:486–501. 2022.PubMed/NCBI View Article : Google Scholar

25 

Webb GI: Naïve Bayes. In: Encyclopedia of Machine Learning. Springer, Boston, MA, pp713-714, 2010.

26 

Jerram ST and Leslie RD: The genetic architecture of type 1 diabetes. Genes (Basel). 8(209)2017.PubMed/NCBI View Article : Google Scholar

27 

Nokoff N and Rewers M: Pathogenesis of type 1 diabetes: Lessons from natural history studies of high-risk individuals. Ann N Y Acad Sci. 1281:1–15. 2013.PubMed/NCBI View Article : Google Scholar

28 

Morahan G: Insights into type 1 diabetes provided by genetic analyses. Curr Opin Endocrinol Diabetes Obes. 19:263–270. 2012.PubMed/NCBI View Article : Google Scholar

29 

Wang Z, Xie Z, Lu Q, Chang C and Zhou Z: Beyond genetics: What causes type 1 diabetes. Clin Rev Allergy Immunol. 52:273–286. 2017.PubMed/NCBI View Article : Google Scholar

30 

Cerna M: Epigenetic regulation in etiology of type 1 diabetes mellitus. Int J Mol Sci. 21(36)2019.PubMed/NCBI View Article : Google Scholar

31 

Stefan M, Zhang W, Concepcion E, Yi Z and Tomer Y: DNA methylation profiles in type 1 diabetes twins point to strong epigenetic effects on etiology. J Autoimmun. 50:33–37. 2014.PubMed/NCBI View Article : Google Scholar

32 

Husseiny MΙ, Kaye A, Zebadua E, Kandeel F and Ferreri K: Tissue-specific methylation of human insulin gene and PCR assay for monitoring beta cell death. PLoS One. 9(e94591)2014.PubMed/NCBI View Article : Google Scholar

33 

Neiman D, Moss J, Hecht M, Magenheim J, Piyanzin S, Shapiro AMJ, de Koning EJP, Razin A, Cedar H, Shemer R and Dor Y: Islet cells share promoter hypomethylation independently of expression, but exhibit cell-type-specific methylation in enhancers. Proc Natl Acad Sci USA. 114:13525–13530. 2017.PubMed/NCBI View Article : Google Scholar

34 

Lehmann-Werman R, Neiman D, Zemmour H, Moss J, Magenheim J, Vaknin-Dembinsky A, Rubertsson S, Nellgård B, Blennow K, Zetterberg H, et al: Identification of tissue-specific cell death using methylation patterns of circulating DNA. Proc Natl Acad Sci USA. 113:E1826–E1834. 2016.PubMed/NCBI View Article : Google Scholar

35 

Kuroda A, Rauch TA, Todorov I, Ku HT, Al-Abdullah IH, Kandeel F, Mullen Y, Pfeifer GP and Ferreri K: Insulin gene expression is regulated by DNA methylation. PLoS One. 4(e6953)2009.PubMed/NCBI View Article : Google Scholar

36 

Herold KC, Usmani-Brown S, Ghazi T, Lebastchi J, Beam CA, Bellin MD, Ledizet M, Sosenko JM, Krischer JP and Palmer JP: Type 1 Diabetes TrialNet Study Group. β cell death and dysfunction during type 1 diabetes development in at-risk individuals. J Clin Invest. 125:1163–1173. 2015.PubMed/NCBI View Article : Google Scholar

37 

Fisher MM, Watkins RA, Blum J, Evans-Molina C, Chalasani N, DiMeglio LA, Mather KJ, Tersey SA and Mirmira RG: Elevations in circulating methylated and unmethylated preproinsulin DNA in new-onset type 1 diabetes. Diabetes. 64:3867–3872. 2015.PubMed/NCBI View Article : Google Scholar

38 

Mouzaki K, Kotanidou EP, Fragou A, Kyrgios I, Giza S, Kleisarchaki A, Tsinopoulou VR, Serbis A, Tzimagiorgis G and Galli-Tsinopoulou A: Insulin gene promoter methylation status in Greek children and adolescents with type 1 diabetes. Biomed Rep. 13(31)2020.PubMed/NCBI View Article : Google Scholar

39 

Mirabello L, Frimer M, Harari A, McAndrew T, Smith B, Chen Z, Wentzensen N, Wacholder S, Castle PE, Raine-Bennett T, et al: HPV16 methyl-haplotypes determined by a novel next-generation sequencing method are associated with cervical precancer. Int J Cancer. 136:E146–E153. 2015.PubMed/NCBI View Article : Google Scholar

40 

Zhao L, Liu D, Xu J, Wang Z, Chen Y, Lei C, Li Y, Liu G and Jiang Y: The framework for population epigenetic study. Brief Bioinform. 19:89–100. 2018.PubMed/NCBI View Article : Google Scholar

41 

Xu J, Zhao L, Liu D, Hu S, Song X, Li J, Lv H, Duan L, Zhang M, Jiang Q, et al: EWAS: Epigenome-wide association study software 2.0. Bioinformatics. 34:2657–2658. 2018.PubMed/NCBI View Article : Google Scholar

42 

Guo S, Diep D, Plongthongkum N, Fung HL and Zhang K and Zhang K: Identification of methylation haplotype blocks aids in deconvolution of heterogeneous tissue samples and tumor tissue-of-origin mapping from plasma DNA. Nat Genet. 49:635–642. 2017.PubMed/NCBI View Article : Google Scholar

43 

Clarke MA, Gradissimo A, Schiffman M, Lam J, Sollecito CC, Fetterman B, Lorey T, Poitras N, Raine-Bennett TR, Castle PE, et al: Human papillomavirus DNA methylation as a biomarker for cervical precancer: Consistency across 12 genotypes and potential impact on management of HPV-positive women. Clin Cancer Res. 24:2194–2202. 2018.PubMed/NCBI View Article : Google Scholar

44 

Xu L, Pen S, He Q, Chen Z and Kuang M: Identification of DNA methylation signatures for microvascular invasion in hepatocellular carcinoma. Gut. 68:A1–A166. 2019.

45 

Peng X, Li Y, Kong X, Zhu X and Ding X: Investigating different DNA methylation patterns at the resolution of methylation haplotypes. Front Genet. 12(697279)2021.PubMed/NCBI View Article : Google Scholar

46 

Hong S, Lin B, Xu M, Zhang Q, Huo Z, Su M, Ma C, Liang J, Yu S, He Q, et al: Cell-free DNA methylation biomarker for the diagnosis of papillary thyroid carcinoma. EBioMedicine. 90(104497)2023.PubMed/NCBI View Article : Google Scholar

47 

Zhang H, Zhang Z, Liu X, Duan H, Xiang T, He Q, Su Z, Wu H and Liang Z: DNA methylation haplotype block markers efficiently discriminate follicular thyroid carcinoma from follicular adenoma. J Clin Endocrinol Metab. 106:1011–1021. 2021.PubMed/NCBI View Article : Google Scholar

48 

Wu H, Guo S, Liu X, Li Y, Su Z, He Q, Liu X, Zhang Z, Yu L, Shi X, et al: Noninvasive detection of pancreatic ductal adenocarcinoma using the methylation signature of circulating tumour DNA. BMC Med. 20(458)2022.PubMed/NCBI View Article : Google Scholar

49 

Mo S, Dai W, Wang H, Lan X, Ma C, Su Z, Xiang W, Han L, Luo W, Zhang L, et al: Early detection and prognosis prediction for colorectal cancer by circulating tumour DNA methylation haplotypes: A multicentre cohort study. EClinicalMedicine. 55(101717)2022.PubMed/NCBI View Article : Google Scholar

50 

Hao Y, Yang Q, He Q, Hu H, Weng Z, Su Z, Chen S, Peng S, Kuang M, Chen Z and Xu L: Identification of DNA methylation signatures for hepatocellular carcinoma detection and microvascular invasion prediction. Eur J Med Res. 27(276)2022.PubMed/NCBI View Article : Google Scholar

51 

de Winter JCF, Dodou D and Wieringa PA: Exploratory factor analysis with small sample sizes. Multivariate Behav Res. 44:147–181. 2009.PubMed/NCBI View Article : Google Scholar

52 

Preacher KJ and MacCallum RC: Exploratory factor analysis in behavior genetics research: Factor recovery with small sample sizes. Behav Genet. 32:153–161. 2002.PubMed/NCBI View Article : Google Scholar

53 

Stephens M, Smith NJ and Donnelly P: A new statistical method for haplotype reconstruction from population data. Am J Hum Genet. 68:978–989. 2001.PubMed/NCBI View Article : Google Scholar

54 

Stephens M and Donnelly P: A comparison of bayesian methods for haplotype reconstruction from population genotype data. Am J Hum Genet. 73:1162–1169. 2003.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Kotanidou EP, Kosvyra A, Mouzaki K, Giza S, Tsinopoulou VR, Serbis A, Chouvarda I and Galli‑Tsinopoulou A: Methylation haplotypes of the insulin gene promoter in children and adolescents with type 1 diabetes: Can a dimensionality reduction approach predict the disease?. Exp Ther Med 26: 461, 2023.
APA
Kotanidou, E.P., Kosvyra, A., Mouzaki, K., Giza, S., Tsinopoulou, V.R., Serbis, A. ... Galli‑Tsinopoulou, A. (2023). Methylation haplotypes of the insulin gene promoter in children and adolescents with type 1 diabetes: Can a dimensionality reduction approach predict the disease?. Experimental and Therapeutic Medicine, 26, 461. https://doi.org/10.3892/etm.2023.12160
MLA
Kotanidou, E. P., Kosvyra, A., Mouzaki, K., Giza, S., Tsinopoulou, V. R., Serbis, A., Chouvarda, I., Galli‑Tsinopoulou, A."Methylation haplotypes of the insulin gene promoter in children and adolescents with type 1 diabetes: Can a dimensionality reduction approach predict the disease?". Experimental and Therapeutic Medicine 26.4 (2023): 461.
Chicago
Kotanidou, E. P., Kosvyra, A., Mouzaki, K., Giza, S., Tsinopoulou, V. R., Serbis, A., Chouvarda, I., Galli‑Tsinopoulou, A."Methylation haplotypes of the insulin gene promoter in children and adolescents with type 1 diabetes: Can a dimensionality reduction approach predict the disease?". Experimental and Therapeutic Medicine 26, no. 4 (2023): 461. https://doi.org/10.3892/etm.2023.12160
Copy and paste a formatted citation
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Spandidos Publications style
Kotanidou EP, Kosvyra A, Mouzaki K, Giza S, Tsinopoulou VR, Serbis A, Chouvarda I and Galli‑Tsinopoulou A: Methylation haplotypes of the insulin gene promoter in children and adolescents with type 1 diabetes: Can a dimensionality reduction approach predict the disease?. Exp Ther Med 26: 461, 2023.
APA
Kotanidou, E.P., Kosvyra, A., Mouzaki, K., Giza, S., Tsinopoulou, V.R., Serbis, A. ... Galli‑Tsinopoulou, A. (2023). Methylation haplotypes of the insulin gene promoter in children and adolescents with type 1 diabetes: Can a dimensionality reduction approach predict the disease?. Experimental and Therapeutic Medicine, 26, 461. https://doi.org/10.3892/etm.2023.12160
MLA
Kotanidou, E. P., Kosvyra, A., Mouzaki, K., Giza, S., Tsinopoulou, V. R., Serbis, A., Chouvarda, I., Galli‑Tsinopoulou, A."Methylation haplotypes of the insulin gene promoter in children and adolescents with type 1 diabetes: Can a dimensionality reduction approach predict the disease?". Experimental and Therapeutic Medicine 26.4 (2023): 461.
Chicago
Kotanidou, E. P., Kosvyra, A., Mouzaki, K., Giza, S., Tsinopoulou, V. R., Serbis, A., Chouvarda, I., Galli‑Tsinopoulou, A."Methylation haplotypes of the insulin gene promoter in children and adolescents with type 1 diabetes: Can a dimensionality reduction approach predict the disease?". Experimental and Therapeutic Medicine 26, no. 4 (2023): 461. https://doi.org/10.3892/etm.2023.12160
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