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Transcriptomic analysis of lipoteichoic acid‑treated undifferentiated and neutrophil‑like differentiated HL‑60 cells

  • Authors:
    • Kuan-Ting Liu
    • I-Jeng Yeh
    • Ya-Ling Hsu
    • Meng-Chi Yen
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    Affiliations: Department of Emergency Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan, R.O.C., Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C.
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 158
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    Published online on: February 22, 2024
       https://doi.org/10.3892/etm.2024.12446
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Abstract

Toll‑like receptor 2 (TLR2) is an important sensor for innate immune cells, including neutrophils, for the recognition of pathogen infection. Lipoteichoic acid (LTA), a cell wall component of gram‑positive bacteria, is a TLR2 ligand. LTA‑induced TLR2 signaling pathways are well established in neutrophils. However, experimental studies regarding transcriptional regulation and the molecular mechanisms in primary human neutrophils are limited due to their short lifespan. The promyelocytic leukemia cell line, HL‑60, can differentiate into a neutrophil‑like phenotype following treatment with dimethyl sulfoxide. The aim of the present study was to investigate whether differentiated HL‑60 (dHL‑60) cells induced a similar gene expression profile upon LTA treatment as that previously determined for primary human neutrophils. After 4 or 24 h of Staphylococcus aureus LTA treatment, undifferentiated HL‑60 (uHL‑60) and dHL‑60 cells were collected for RNA sequencing. The results demonstrated that hundreds of identical differentially expressed genes (DEGs) were observed in 1 and 10 µg/ml LTA‑treated dHL‑60 cells following 4 and 24 h of incubation, while almost no DEGs between LTA‑treated HL‑60 and dHL‑60 cells were observed. Using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses (KEGG), it was noted that the pathways of shared DEGs between the 1 and 10 µg/ml LTA‑treated dHL‑60 cells at both time points were significantly enriched in immune and inflammatory response‑related pathways, such as cellular response to tumor necrosis factor, interleukin‑1, interferon γ, neutrophil chemotaxis, the NF‑κB signaling pathway and the Toll‑like receptor signaling pathway. In addition, when comparing the effect of 1 and 10 µg/ml LTA treatment on dHL60 cells, it was found that all enriched GO and KEGG pathways were associated with the TLR signaling pathways of neutrophils. The results of the present study provided important information for the implementation of mRNA profiling in LTA‑treated dHL‑60 cells and may indicate the feasibility of using dHL‑60 cells as a research model for TLR2 signaling in human neutrophils.
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Copy and paste a formatted citation
Spandidos Publications style
Liu K, Yeh I, Hsu Y and Yen M: Transcriptomic analysis of lipoteichoic acid‑treated undifferentiated and neutrophil‑like differentiated HL‑60 cells. Exp Ther Med 27: 158, 2024.
APA
Liu, K., Yeh, I., Hsu, Y., & Yen, M. (2024). Transcriptomic analysis of lipoteichoic acid‑treated undifferentiated and neutrophil‑like differentiated HL‑60 cells. Experimental and Therapeutic Medicine, 27, 158. https://doi.org/10.3892/etm.2024.12446
MLA
Liu, K., Yeh, I., Hsu, Y., Yen, M."Transcriptomic analysis of lipoteichoic acid‑treated undifferentiated and neutrophil‑like differentiated HL‑60 cells". Experimental and Therapeutic Medicine 27.4 (2024): 158.
Chicago
Liu, K., Yeh, I., Hsu, Y., Yen, M."Transcriptomic analysis of lipoteichoic acid‑treated undifferentiated and neutrophil‑like differentiated HL‑60 cells". Experimental and Therapeutic Medicine 27, no. 4 (2024): 158. https://doi.org/10.3892/etm.2024.12446
Copy and paste a formatted citation
x
Spandidos Publications style
Liu K, Yeh I, Hsu Y and Yen M: Transcriptomic analysis of lipoteichoic acid‑treated undifferentiated and neutrophil‑like differentiated HL‑60 cells. Exp Ther Med 27: 158, 2024.
APA
Liu, K., Yeh, I., Hsu, Y., & Yen, M. (2024). Transcriptomic analysis of lipoteichoic acid‑treated undifferentiated and neutrophil‑like differentiated HL‑60 cells. Experimental and Therapeutic Medicine, 27, 158. https://doi.org/10.3892/etm.2024.12446
MLA
Liu, K., Yeh, I., Hsu, Y., Yen, M."Transcriptomic analysis of lipoteichoic acid‑treated undifferentiated and neutrophil‑like differentiated HL‑60 cells". Experimental and Therapeutic Medicine 27.4 (2024): 158.
Chicago
Liu, K., Yeh, I., Hsu, Y., Yen, M."Transcriptomic analysis of lipoteichoic acid‑treated undifferentiated and neutrophil‑like differentiated HL‑60 cells". Experimental and Therapeutic Medicine 27, no. 4 (2024): 158. https://doi.org/10.3892/etm.2024.12446
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